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| Effect of absence of natural killer cells and CD8+T cells on liver function in mice infected with metacestode of Echinococcus multilocularis |
| Ayinuer Aierken1 LUO Li1 Abuduaini Abulizi1 Abuduhaiwaier Abuduhelili1 Talaiti Tuergan1 Tuerganaili Aji2 |
1.Department of Hepatobiliary & Hydatid Disease, Digestive & Vascular Surgery Center, First Affiliated Hospital of Xinjiang Medical University, Xinjiang Uygur Autonomous Region, Urumqi 830054, China;
2.State Key Laboratory of Pathogenesis, Prevention and Treatment of High Incidence Diseases in Central Asia, Xinjiang Uygur Autonomous Region, Urumqi 830054, China
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Abstract Objective To observe the effect of the absence of natural killer (NK) cells and CD8+T cells on the liver function in mice infected with metacestode of Echinococcus multilocularis. Methods A total of 30 SPF grade female C57BL/6 mice aged six to eight weeks and with a body weight of 18-20 g were selected and divided into control group, metacestode of Echinococcus multilocularis infection group, NK cell clearance group, NK cell clearance control group, CD8+T cell clearance group, and CD8+T cell clearance control group according to random number table method, with five mice in each group. Metacestode of Echinococcus multilocularis infection group, NK cell clearance group, NK cell clearance control group, CD8+T cell clearance group, CD8+T cell clearance control group were all injected into the portal vein with the scolices of Echinococcus multilocularis Leuckart (10 scolices/μl, 200 μl), and the control group was injected into the portal vein with equal amount of normal saline. At the fourth week of the experiment, the NK cell clearance group was intraperitoneally injected with NK cell clearance antibody, 100 μg each time, once a week, while the NK cell clearance control group was intraperitoneally injected with the same amount of IgG2a homologous control antibody; CD8+T cell clearance group was intraperitoneally injected with CD8+T cell clearance antibody 200 μg each time, once a week, while CD8+T cell clearance control group was intraperitoneally injected with the same amount of IgG2b homologous control antibody. At the 12th week of the experiment, the peripheral blood of six groups was collected, the peripheral blood plasma was separated, and the liver and kidney function indexes of total protein (TP), alkaline phosphatase, glutamic-pyruvic transaminase (GPT), glutamic-oxaloacetic transaminase (GOT), albumin (ALB), lactate dehydrogenase, uric acid, urea, and creatinine were detected. Results The levels of GPT and GOT in the peripheral blood of metacestode of Echinococcus multilocularis infection group were higher than those of control group, and the differences were statistically significant (P＜0.05 or P＜0.01). The levels of GPT and GOT in peripheral blood of NK cell clearance group were higher than those of NK cell clearance control group, and TP and ALB were lower than those of NK cell clearance control group, the differences were statistically significant (P＜0.05 or P＜0.01). The level of TP inperipheral blood of CD8+T cell clearance group was lower than that of CD8+T cell clearance control group, and the difference was statistically significant (P＜0.05). Conclusion Metacestode of Echinococcus multilocularis infection can cause liver function damage to a certain extent, especially in the state of NK cell depletion.
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