Abstract Objective To investigate the role of macrophage migration inhibitory factor (MIF) in early brain injury (EBI) after subarachnoid hemorrhage (SAH) in rats. Methods A total of 120 12-month-old healthy male Sprague Dawley rats (clean grade) were divided into Sham group, SAH group and inhibitor group according to random number table method, with 40 rats in each group. 0.5 h before operation, rats in the inhibitor group were intraperitoneally injected with MIF inhibitor (10 mg/kg), and rats in the SAH group and Sham group were intraperitoneally injected with 0.9% sodium chloride injection, and then SAH group and inhibitor group were constructed by secondary blood injection in the occipital cisterna. In the Sham group, 0.9% sodium chloride injection was injected with the same amount of liquid twice. Cortical MIF protein, interleukin-1β (IL-1β), tumor necrosis factor-α (TNF-α), IL-6, nerve cell apoptosis, brain water content, blood-brain barrier permeability and neurobehavioral function were determined after surgery. Results Compared with Sham group, MIF protein, IL-1β, TNF-α, IL-6, proportion of apoptotic nerve cells, brain water content index and content of Evan’s blue of rats in SAH group were significantly increased, and neurobehavioral scores were significantly decreased (P＜0.05). Compared with SAH group, IL-1β, TNF-α, IL-6, proportion of apoptotic nerve cells, brain water content index and content of Evan’s blue in inhibitor group were significantly decreased, and neurobehavioral scores were significantly increased (P＜0.05), but there was no statistical significance in MIF protein level between SAH group and inhibitor group (P＞0.05). Conclusion MIF may induce EBI after SAH, and inhibition of MIF may protect EBI.
SHENTU Huasong CHEN Yihua CHENG Zhenyu. Role of macrophage migration inhibitor in early brain injury after subarachnoid hemorrhage in rats[J]. 中国医药导报, 2023, 20(21): 14-18.
