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| Construction of apoptosis-related lncRNA-mRNA network in ischemic stroke and prediction of hub genes |
| GU Lian1 YANG Yibing2 YAN Yan1 HUANG Xianli2 SU Li3 LIANG Baoyun1 |
1.Ward Two of Encephalopathy, the First Affiliated Hospital of Guangxi University of Chinese Medicine, Guangxi Zhuang Autonomous Region, Nanning 530022, China;
2.Graduate School, Guangxi University of Chinese Medicine, Guangxi Zhuang Autonomous Region, Nanning 530001, China;
3.Public Health School, Guangxi Medical University, Guangxi Zhuang Autonomous Region, Nanning 530021, China
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Abstract Objective To explore the apoptosis-related long non-coding RNA(lncRNA)-mRNA network in ischemic stroke (IS) and predict the hub genes. Methods A total of 10 patients with IS admitted to the First Affiliated Hospital of Guangxi University of Chinese Medicine (“our hospital” for short) from January 2016 to June 2017, and 10 healthy controls from community health examination center of our hospital during the same period were selected as the research objects. Differential analysis of microarray expression profiles was performed, weighted gene co-expression network analysis was used to screen out genes in the target module, and the intersection with apoptotic genes was taken. Top 10 differentially mRNAs (DEMs) were selected using the STRING website and Cytoscape software, and lncRNA-mRNA co-expression network was constructed between DEMs and differentially lncRNAs (DELs). Finally, hub genes were obtained. Results A total of 3 930 DEMs were obtained, and 553 apoptosis-related IS genes were obtained after intersection of module genes and apoptosis genes. The lncRNA-mRNA co-expression network consisted of 9 DEMs and 277 DELs. Finally, 7 hub genes G015366, G068382, PIK3CB, BPTF, NR3C1, Akt2, and INS were obtained. Conclusion G015366, G068382, PIK3CB, BPTF, NR3C1, Akt2, and INS are related to the occurrence and development of IS, and may become diagnostic markers of IS.
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