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| Study of optimization and activity of extraction process of fermented mulberry leaf glycoprotein |
| SUN Dandan SUN Yinling ZHENG Hongyu DING Chunjie ZHAO Han SONG Jipeng CHEN Liyan |
Institute of Chinese Materia Medica, Heilongjiang Academy of Traditional Chinese Medicine, Heilongjiang Province, Harbin 150036, China
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Abstract Objective To determine the optimal extraction process of fermented mulberry leaf glycoprotein (FMLGP), and to evaluate the activity of glycoprotein. Methods Taking protein concentration as index, the extraction process of FMLGP was optimized by single factor combined with response surface method. Fibrinolytic activity was detected by agarose fibrin plate method. Macrophage RAW264.7 foam was induced by ox-LDL. The experiment was divided into blank group, model group, and FMLGP high-dose, medium-dose, and low-dose groups (the final concentration was 15, 10, and 5 mg/ml, respectively). The cells were cultured for 24 h and the degree of lipid accumulation in each group was investigated by Oil red staining. Results The optimal extraction conditions of FMLGP were PBS buffer solution, solid-liquid ratio of 1∶6 g/ml, extraction at 25℃ for 4 h, protein concentration of (57.92±1.14) mg/ml, fibrinolytic activity of (1 780.66±76.49) IU/ml, and 15 mg/ml FMLGP could significantly promote lipid excretion in macrophage foam cells. Conclusion In this study, the optimal extraction process of FMLGP is determined, and the fibrinolytic activity and macrophage foaming experiments shows that FMLGP had potential anti-atherosclerosis effects.
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