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| Effect of the acupuncture and moxibustion in the neurological function and treatment of injury in rats with acute intracranial hemorrhage |
| MA Xuemiao1 DU Ye2 CHENG Guangyu3 CHENG Weiping4 |
1.School of Graduate, Heilongjiang University of Chinese Medicine, Heilongjiang Province, Harbin 150040, China;
2.Department of Endocrinology, the First Affiliated Hospital of Heilongjiang University of Chinese Medicine, Heilongjiang Province, Harbin 150040, China; 3.Department of Perception and Control, the First Affiliated Hospital of Heilongjiang University of Chinese Medicine, Heilongjiang Province, Harbin 150040, China;
4.Department of Neurology, the First Affiliated Hospital of Heilongjiang University of Traditional Chinese Medicine, Heilongjiang Province, Harbin 150040, China
[Abstract] |
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Abstract Objective To observe the improvement effect of acupuncture on the neurological function and craniocerebral injury in rats with acute intracranial hemorrhage. Methods A total of 45 healthy male SD rats of about 220 g in 6-8 weeks were selected, and they were divided into sham operation group, model group and acupuncture group by random number table method, with 15 rats in each group; each group was divided into three points at 1, 3 d and 7 d, with 5 rats in each time. The cerebral hemorrhage rat model group was established by autologous blood injection; the sham operation group only drilled holes without injecting autologous blood; the acupuncture group adopted the treatment method of piercing from Baihui to Qubin. Ludmila Belayev’s neurological function score was used to evaluate the degree of neurological deficit in rats in each group. Western blot was used to detect the expression of CTRP3 and GPX4 proteins in brain tissue. The mitochondrial morphology of nerve cells in the brain tissue of rats in each group was observed by transmission electron microscope. qRT-PCR method was used to detect the specific expression of GFAP and EGFR mRNA in rat brain tissue. Results Compared with sham operation group, neurological function deficit score in model group increased at each time point (P＜0.05). Compared with model group, neurological impairment scores of acupuncture group decreased at 1 and 3 d, the differences were statistically significant (P＜0.05), but there was no statistically significant difference in systemic impairment score at 7 d(P＞0.05). Compared with sham operation group, levels of CTRP3 and GPX4 protein in model group were decreased at each time point, and the differences were statistically significant (P＜0.05). Compared with model group, CTRP3 and GPX4 protein expression levels in acupuncture group were up-regulated at each time point, and the differences were statistically significant (P＜0.05). Compared with sham operation group, mRNA levels of GFAP and EGFR in brain tissue of rats in model group increased at each time point, and the differences were statistically significant (P＜0.05). Compared with model group, mRNA levels of GFAP and EGFR in brain tissue of rats in acupuncture group were significantly decreased at each time point, and the differences were statistically significant (P＜0.05). Conclusion Acupuncture treatment can up-regulate the expression of CTRP3 and GPX4 proteins in brain tissue, reduce the mRNA levels of GFAP and EGFR, and restore the integrity of neuronal mitochondria, thereby improving the neuromuscular injury and neurological function of rats with acute cerebral hemorrhage, and play a protective role.
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