5-氮杂-2’-脱氧胞苷对苯并[a]芘致海马神经元毒性的保护作用

doi:10.20047/j.issn1673-7210.2023.16.05

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Abstract Objective To probe the protective effects of 5-Aza-2’-deoxycytidine (5-Aza-cdR) on the neurotoxicity of benzo[a] pyrene(B[a]P) to hippocampal neurons. Methods The hippocampal neurons were cultured until the 5th day. The cells were observed and identified. The experiment was divided into control group, B[a]P group(20 μmol/L, B[a]P), and B[a]P+5-Aza-cdR group (20 μmol/L B[a]P+10 μmol/L 5-Aza-cdR). Cell morphology was observed. Cell viability, DNA methyltransferase protein， and gene expression were detected. Total methylation level and apoptosis were measured. Results Compared with the control group, the B[a]P group decreased the viability of hippocampal neurons(P＜0.05). The apoptosis rate increased (P＜0.05). The expression level of DNMT3A, DNMT3B gene and DNMT1、DNMT3A protein increased (P＜0.05). The total methylation level increased (P＜0.05). Compared with the B[a]P group, the B[a]P+5-Aza-cdR group increased hippocampal neuronal viability (P＜0.05), and the apoptosis rate decreased (P＜0.01) and visibly decreased DNMTs gene and protein expression levels (P＜0.05). The methylation level was obviously decreased (P＜0.01). Conclusion 5-Aza-cdR has a protective effect on B[a] P-induced hippocampal neuron injury.

Key words： Benzo[a]pyrene 5-Aza-2’-deoxycytidine Hippocampal neurons Methylation

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	WEI Zhichao1 FENG Jingyu2 NIE Jisheng1 WEI Jianhong2

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WEI Zhichao1 FENG Jingyu2 NIE Jisheng1 WEI Jianhong2. Protective effects of 5-Aza-2’-deoxycytidine on neurotoxicity of hippocampal neurons induced by benzo[a]pyrene[J]. 中国医药导报, 2023, 20(16): 26-30.

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