|
|
|
| Effects of vitamin D intervention on balance regulation of peripheral blood T lymphocytes and intestinal flora in young rats with bronchial asthma |
| CAI Zixiu1 ZHOU Guohua1 TIAN Jun2▲ |
1.Department of Pediatrics, the First People’s Hospital of Xiaoshan District, Hangzhou City, Zhejiang Province, Hangzhou 311200, China;
2.Department of Pediatrics, the Third People’s Hospital of Xiaoshan District, Hangzhou City, Zhejiang Province, Hangzhou 311256, China
|
|
|
|
Abstract Objective To investigate the effects of vitamin D (VitD) intervention on the balance regulation of peripheral blood T lymphocytes and intestinal flora in young rats with bronchial asthma (hereinafter referred to as “asthma”). Methods Thirty SPF wild-type BALB/c female mice (4.09 - 6.13 g) aged seven days were selected as the study objects, and they were divided into asthma model group (15 rats) and VitD intervention group (15 rats) by random number table method, another ten rats were selected as healthy control group. On the first and seventh day of the experiment, asthma model group and VitD intervention group were injected intraperitoneally with 10% ovalbumin (OVA) sensitizing solution 200 μl, while healthy control group was injected with the same amount of normal saline. On the 18th day, VitD intervention group was given VitD drops (35 μg/kg, per week, one every two days) for two weeks, and healthy control group and asthma model group were given the same amount of phosphate buffer. On the 19th day, asthma model group and VitD intervention group were given atomized inhalation of 3%OVA solution 10 ml for 20 min, while healthy control group was given atomized inhalation of normal saline, once a day for six days. After the experiment, the hematoxylin-eosin staining results of lung tissue sections in three groups were observed. Serum Th1/Th2 and Th17/ regulatory T cell (Treg) ratios were measured and calculated by flow cytometry. The bacterial community in feces of the three groups was analyzed by 16S rDNA sequencing method. Results In the healthy control group, the structure of the bronchial wall and alveolar wall of the lung tissue were complete, there was no exudation in the alveolar cavity, the alveolar epithelium was orderly, and the alveolar wall thickness was normal. In asthmatic model group, the structure of alveolar wall was damaged, alveolar epithelial cells were destroyed, mucosal hyperemia and edema were observed, and perivascular inflammatory cells were infiltrated. The inflammatory reaction of lung tissue in VitD intervention group was less. The Shannon index curve of microbial species in the three groups was flat, and there was no statistical significance in Simpson index among the three groups (P＞0.05). PCoA diagram analysis showed that the distance between the asthma model group and the healthy control group was large, and the distance between the VitD intervention group and the asthma model group was close (P＜0.05). The three groups of intestinal flora involved 15 divisions and 23 genera. Th1/Th2 ratio and the abundance of Actinobacteriota, Helicobacter, and Colidextribacter in asthma model group were lower than those in healthy control group, Th17/Treg ratio and the abundance of Proteobacteria, Ligilactobacillus, and Bacteroides of asthma model group were higher than those of healthy control group (P＜0.05). Th1/Th2 ratio and the abundance of Actinobacteriota, Proteobacteria, Helicobacter, and Colidextribacter in VitD intervention group were higher than those in asthma model group, The Th17/Treg ratio and the abundance of Ligilactobacillus, Bacteroides in VitD intervention group were lower than those of the asthma model group (P＜0.05), which were consistent with the result of heatmap analysis based on genera level. Conclusion VitD intervention can regulate the balance of peripheral blood T lymphocytes and change the intestinal flora of young mice with asthma, which may be a new entry point for the pathogenesis of this disease.
|
|
|
|
|
|
[1] 宋芬乐，成焕吉，李艳春，等.支气管哮喘儿童哮喘控制与治疗现状的临床观察[J].中国妇幼保健，2016，31（9）：1891-1893.
[2] Pike KC，Levy ML，Moreiras J，et al. Managing problematic severe asthma：beyond the guidelines [J]. Arch Dis Child，2018，103（4）：392-397.
[3] 余其梅，林慧，叶方立，等.血清维生素D水平与成人哮喘严重程度关系的Meta分析[J].实用医学杂志，2019，35（15）：2407-2412.
[4] 石聪聪，王小稳，杨德彬.辅助性T淋巴细胞亚群、维生素D在儿童难治性哮喘中的表达研究[J].医药论坛杂志，2021，42（18）：22-25.
[5] 沙影丽，姜慧轶，闫雯雯.婴幼儿哮喘血清维生素D及T细胞亚群变化研究[J].长春中医药大学学报，2019，35（6）： 1142-1145.
[6] 张志华，肖晓晨，梅少奇.维生素D3辅助治疗哮喘合并呼吸道感染对气道重塑及免疫功能影响[J].中华肺部疾病杂志（电子版），2022，15（3）：355-357.
[7] 胡翠萍.维生素D对哮喘儿童Th1/Th2/Th17/Treg免疫失衡的影响研究[J].中国预防医学杂志，2019，20（4）：357- 360.
[8] 吕晓丽.早期维生素D补充对儿童生长发育及肠道菌群的影响[J].中国处方药，2022，20（9）：108-110.
[9] 石山领，张晓宇.肠道菌群与支气管哮喘发病相关机制研究进展[J].中华实用诊断与治疗杂志，2015，35（2）：200-202.
[10] Ver Heul A，Planer J，Kau AL. The Human Microbiota and Asthma [J]. Clin Rev Allergy Immunol，2019，57（3）：350- 363.
[11] 王剑清，刘艳明，罗兴华.维生素D对肥胖幼鼠单核／巨噬细胞趋化因子及巨噬细胞浸润脂肪组织的影响[J].中华实用儿科临床杂志，2017，32（19）：1484-1489.
[12] 宋玮，李英，宋琼珠，等.哮喘患者Th1/Th2细胞因子水平变化及其临床意义[J].热带医学杂志，2022，22（7）：974-978.
[13] 王开梅，林志雄，胡祥英，等.呼吸道病毒感染对支气管哮喘患儿Th1/Th2细胞因子平衡的影响[J].中国医药导报，2019，16（5）：76-79.
[14] Ogeyingbo OD，Ahmed R，Gyawali M，et al. The Relationship Between Vitamin D and Asthma Exacerbation [J]. Cureus，2021，13（8）：1-7.
[15] Solidoro P，Bellocchia M，Aredano I，et al. Asthmatic Patients with Vitamin D Deficiency have Decreased Exacerbations after Vitamin Replacement [J]. Nutrients，2017，9（11）：1-14.
[16] 刘瑞芳，张蓉芳，李桂荣，等.维生素D受体基因多态性与哮喘相关性meta分析[J].国际呼吸杂志，2019，39（7）：487-494.
[17] Luo J，Liu D，Liu CT. Can Vitamin D Supplementation in Addition to Asthma Controllers Improve Clinical Outcomes in Patients With Asthma？A Meta-Analysis [J]. Medicine（Baltimore），2015，94（50）：1-10.
[18] Hillman ET，Lu H，Yao T，et al. Microbial Ecology along the Gastrointestinal Tract [J]. Microbes Environ，2017，32（4）：300-313.
[19] 王静，沈红莲，孙建华.肠道菌群失调和免疫失衡与儿童支气管哮喘的关系[J].中国微生态学杂志，2022，34（10）：1205-1208.
[20] 崔天怡，刘佳蕊，吕彬，等.肠道菌群及免疫调节与儿童哮喘关系的研究进展[J].中国全科医学，2022，25（8）：1021-1026.
[21] 杨洁梅，杨巧红.维生素D对婴幼儿过敏性疾病影响的研究进展[J].实用临床护理学电子杂志，2019，4（47）：181-183.
[22] 刘素琴，李燕，王磊，等.婴幼儿反复呼吸道感染与血清25羟维生素D水平及体外过敏原、总IgE的关系[J].中国医药导报，2022，19（35）：88-91.
[23] 祝海波，周苗苗，王海，等.婴儿早期维生素D补充水平对生长发育和肠道菌群的影响[J].中国食物与营养，2018，24（12）：87-89.
[24] Bellerba F，Muzio V，Gnagnarella P，et al. The Association between Vitamin D and Gut Microbiota：A Systematic Review of Human Studies [J]. Nutrients，2021，13（10）：3378.
[25] 李了，杨磊.肺和肠道微生物群落与哮喘的研究进展[J].中国医药导报，2022，19（17）：42-45.
[26] 吴盛桥，区发强，黄建聪.布地奈德联合维生素D治疗儿童支气管哮喘的疗效[J].中国医药科学，2021，11（1）：141-143，147.
[27] 邱萌.支气管哮喘患儿血清维生素D与气道呼吸参数关系及对预后的影响[J].河北医学，2021，27（8）：1339-1343.
[28] 袁路，袁杰力，文姝，等.维生素D辅助治疗过敏性哮喘的研究进展[J].中国微生态学杂志，2021，33（6）：741- 745. |
|
|
|
