两性霉素B对假丝酵母菌与乳杆菌生物膜作用的研究

doi:10.20047/j.issn1673-7210.2023.16.01

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Abstract Objective To study the effect of amphotericin B on Candida and Lactobacillus biofilm. Methods From July 2022 to August 2022, four strains of Candida and four strains of Lactobacillus from the vagina of patients in Beijing Obstetrics and Gynecology Hospital, Capital Medical University were selected to determine their biofilm formation ability at different stages and observe their biofilm formation. The minimum inhibitory concentration (MIC) and minimum biofilm inhibitory concentration (MBIC) of amphotericin B on Candida and lactobacillus were determined respectively, and the effect of amphotericin B on the biofilms was observed under scanning electron microscope. Results The biofilm formation ability of Candida and Lactobacillus at 48 h was higher than that at 24 h (P＜0.05), and the biofilm formation ability of Lactobacillus No.7 at 48 h was higher than that at 72 h (P＜0.05). The MIC of amphotericin B against Candida albicans was 0.125 00 μg/ml. When the concentration of amphotericin B was 2 μg/ml, the biofilm forming ability of Candida No.1, No.3 and No.4 was lower than that of the positive control group (amphotericin B 0 μg/ml)(P＜0.05), and there was no difference between them and the subsequent concentrations (P＞0.05), the MBIC of amphotericin B against Candida albicans was 2 μg/ml. Amphotericin B had no inhibitory effect on Lactobacillus crispatus. The biofilm forming ability of Lactobacillus No.7 at concentration of 1, 8, and 16 μg/ml was higher than that of positive control group (P＜0.05), and the other Lactobacillus crispatus had no difference in biofilm forming ability at different concentrations (P＞0.05). Conclusion Candida albicans and Lactobacillus crispatus had the strongest biofilm forming ability at 48 h. Amphotericin B has strong antibacterial ability to Candida albicans, but it needs a larger dose to destroy the biofilms of Candida albicans which have formed. It has no inhibitory effect on Lactobacillus crispatus and biofilms destructive effect.

Key words： Amphotericin B Candida Lactobacillus Biofilm

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	SHANG Xiang1 BAI Huihui2 LIU Zhaohui1▲

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SHANG Xiang1 BAI Huihui2 LIU Zhaohui1▲. Study on the effect of amphotericin B on the biofilm of Candida and Lactobacillus[J]. 中国医药导报, 2023, 20(16): 4-10.

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https://www.yiyaodaobao.com.cn/EN/10.20047/j.issn1673-7210.2023.16.01 OR https://www.yiyaodaobao.com.cn/EN/Y2023/V20/I16/4

[1] Workowski KA，Bachmann LH，Chan PA，et al. Sexually Tra- nsmitted Infections Treatment Guidelines，2021 [J]. MMWR Recomm Rep，2021，70（4）：1-187.
[2] Farr A，Effendy I，Frey Tirri B，et al. Guideline：Vulvovaginal Candidosis （AWMF 015/072，Level S2k）[J]. Mycoses，2021（1）：1-20.
[3] American College of Obstetricians and Gynecologists. Vagi- nitis in Nonpregnant Patients：ACOG Practice Bulletin No.215 [J]. Obstet Gynecol，2020，135（1）：e1-e17.
[4] Chen M，Xu Y，Hong N，et al. Epidemiology of Fungal Infect- ions in China [J]. Front Med，2018，12（1）：58-75.
[5] Wall G，Montelongo-Jauregui D，Vidal Bonifacio B，et al. Candida Albicans Biofilm Growth and Dispersal：Contributions to Pathogenesis [J]. Curr Opin Microbiol，2019，52：1-6.
[6] Tran KD，Aldrich BT，D’Amato Tóthová J，et al. Efficacy and Safety of Various Amphotericin B Concentrations on Candida Albicans in Cold Storage Conditions [J]. Cornea，2020， 39（1）：110-117.
[7] 霍丽丽，刘朝晖.两性霉素B对阴道耐药假丝酵母菌药物敏感研究[J].中国实用妇科与产科杂志，2016，32（7）：667-670.
[8] Dantas-Medeiros R，Zanatta AC，de Souza LBF，et al. Ant- ifungal and Antibiofilm Activities of B-Type Oligomeric Procyanidins From Commiphora Leptophloeos Used Alone or in Combination With Fluconazole Against Candida Spp [J]. Front Microbiol，2021，12：613155.
[9] Shiroda M，Manning SD. Lactobacillus Strains Vary in Their Ability to Interact with Human Endometrial Stromal Cells [J]. PLoS One，2020，15（9）：1-15.
[10] CLSI. Reference Method for Broth Dilution Antifungal Susceptibility Testing of Yeasts [M]. CLSI standard M27 4th edition. Wayne，PA：Clinical and Laboratory Standards Institute，2017：1-23.
[11] Willems HME，Ahmed SS，Liu J，et al. Vulvovaginal Candidiasis：A Current Understanding and Burning Questions [J]. J Fungi(Basel)，2020，6（1）：27.
[12] Chen H，Zhou X，Ren B，et al. The Regulation of Hyphae Growth in Candida Albicans [J]. Virulence，2020，11（1）：337-348.
[13] Mukaremera L，Lee KK，Mora-Montes HM，et al. Candida Albicans Yeast，Pseudohyphal，and Hyphal Morphogenesis Differentially Affects Immune Recognition [J]. Front Immunol，2017，8：629.
[14] Pierce CG，Vila T，Romo JA，et al. The Candida Albicans Biofilm Matrix：Composition，Structure and Function [J]. J Fungi （Basel），2017，3（1）：14.
[15] Pappas PG，Kauffman CA，Andes DR，et al. Executive Sum- mary：Clinical Practice Guideline for the Management of Candidiasis：2016 Update by the Infectious Diseases Society of America [J]. Clin Infect Dis，2016，62（4）：409-417.
[16] Zielińska J，Wieczór M，Chodnicki P，et al. Self-Assembly，Stability and Conductance of Amphotericin B Channels：Bridging the Gap between Structure and Function [J]. Nan- oscale，2021，13（6）：3686-3697.
[17] Silva LN，Oliveira SSC，Magalh?觔es LB，et al. Unmasking the Amphotericin B Resistance Mechanisms in Candida Haemulonii Species Complex [J]. ACS Infect Dis，2020，6（5）：1273- 1282.
[18] Von Lilienfeld-Toal M，Wagener J，Einsele H，et al. Invasive Fungal Infection [J]. Dtsch Arztebl Int，2019，116（16）： 271-278.
[19] Melo CM，Cardoso JF，Perassoli FB，et al. Amphotericin B- Loaded Eudragit RL100 Nanoparticles Coated with Hyalur- onic Acid for the Treatment of Vulvovaginal Candidiasis [J]. Carbohydr Polym，2020，230：115608.
[20] Borman AM，Muller J，Walsh-Quantick J，et al. MIC Distributions for Amphotericin B，Fluconazole，Itraconazole，Voriconazole，Flucytosine and Anidulafungin and 35 Uncommon Pathogenic Yeast Species from the UK Determined Using the CLSI Broth Microdilution Method [J]. J Antimicrob Chemother，2020，75（5）：1194-1205.
[21] Lee H，Choi SH，Oh J，et al. Comparison of Six Antifungal Susceptibilities of 11 Candida Species Using the VITEK2 AST-YS08 Card and Broth Microdilution Method [J]. Microbiol Spectr，2022，10（2）：e01253-21.
[22] Kwon MS，Lee HK. Host and Microbiome Interplay Shapes the Vaginal Microenvironment [J]. Front Immunol，2022， 13：919728.
[23] Jeng H，Yan T，Chen J. Treating Vaginitis with Probiotics in Non-pregnant Females：A Systematic Review and Meta- analysis [J]. Exp Ther Med，2020，20（4）：3749-3765.
[24] Matsubara VH，Bandara HMHN，Mayer MPA，et al. Probiotics as Antifungals in Mucosal Candidiasis [J]. Clin Infect Dis，2016，62（9）：1143-1153.
[25] Oliveira VMC，Santos SSF，Silva CRG，et al. Lactobacillus Is Able to Alter the Virulence and the Sensitivity Profile of Candida Albicans [J]. J Appl Microbiol，2016，121（6）：1737- 1744.
[26] 陈曦，刘朝晖.乳杆菌制剂与抗真菌药物联合及序贯应用治疗重度外阴阴道假丝酵母菌病疗效研究[J].中国实用妇科与产科杂志，2017，33（3）：293-296.