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| Screening of excellent strains derived from 7 β，15 α-dihydroxy-18β- glycyrrhetinic acid by microbial transformation |
| MA Yuan1 SUN Peng2 WANG Heng3 LI Zhuofan3 CHEN Gang4 SHI Minghui4 |
1.Key Laboratory of Uygur Medicines, Xinjiang Uygur Autonomous Region Institute of Materia Medica, Xinjiang Uygur Autonomous Region, Urumqi 830004, China;
2.Medical Device Testing Center, Xinjiang Institutes of Drug Control, Xinjiang Uygur Autonomous Region, Urumqi 830054, China;
3.Department of Traditional Chinese Medicine, College of Pharmacy, Shihezi University, Xinjiang Uygur Autonomous Region, Shihezi 832000, China; 4.Analysis and Testing Center, Xinjiang Uygur Autonomous Region Institute of Traditional Chinese Medica and National Medica, Xinjiang Uygur Autonomous Region, Urumqi 830002, China
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Abstract Objective To screen the excellent strains through microbial transformation, using 7β，15α-dihydroxy-18β- glycyrrhetinic acid as the target product. Methods Ten fungi (CGMCC 3.149, CGMCC 3.193, CGMCC 3.3402, CGMCC 3.739, CGMCC 3.2462, CGMCC 3.2886, CGMCC 3.3400, CGMCC 3.577, CGMCC 3.970, and Zw-4) were used for preliminary screening and re-screening experiments of transformed strains, the content of 7β，15α-dihydroxy-18β-glycyrrhetinic acid in the converted liquid was determined by high performance liquid chromatography. The conversion rate of 7β，15α-dihydroxy-18β-glycyrrhetinic acid was used as the evaluation index and the strains with high conversion rate and consistent results of preliminary screening and re-screening experiments were selected as the excellent strains. 7β，15α-dihydroxy- 18β-glycyrrhetinic acid showed a good linear relationship in the range of 2.56~81.79 μg/ml. The strain with the highest conversion rate of preliminary screening experiment was CGMCC 3.3400. The strain with the highest conversion rate of re-screening experiment was CGMCC 3.970. Conclusion The excellent strain is CGMCC 3.970.
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