新疆阿魏根提取物对白细胞介素-22诱导的角质形成细胞异常增殖的作用研究

doi:10.20047/j.issn1673-7210.2023.14.03

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Abstract Objective To investigate the effects of different Ferula Sinkiang root extract on the abnormal proliferation of HaCaT cells, induced by interleukin-22（IL-22） and to provide evidence for evaluating the mechanism of action of Radix Aprofunda extract on HaCaT cells in psoriasis. Methods Experimental group: blank control group, IL-22 experimental group, ferulic acid low-dose, medium-dose and high-dose groups (0.5, 1, 2 mmol/L), sesquiterpenoid low-dose, medium-dose and high-dose groups (5, 10, 15 mg/ml), except blank control group, after 24 h intervention with 100 ng/ml IL-22, the cells were cultured for 24 h by adding 100 μl of different concentrations to observe cell morphology. Cell proliferation was detected by CCK8 assay. The cell cycle of each group was detected by flow cytometry. The contents of tumor necrosis factor-α（TNF-α），interferon-γ（IFN-γ）, IL-23 and IL-4 in the supernatant of cultured cells were determined by enzyme-linked immunosorbent assay. Results Ferulic acid had no significant effect on the growth state of HaCaT cells, but sesquiterpenoids could inhibit the excessive proliferation of Hacat cells. There was no significant difference in cell proliferation rate between ferulic acid dose groups and IL-22 group (P＞0.05). The proliferation rate of HaCaT cells in sesquiterpene dose groups was significantly different compared with that that in IL-22 experimental group (P＜0.05). There was no significant difference in cell cycle between ferulic acid groups and IL-22 experimental group (P＞ 0.05). The percentages of G0/G1 phase, and G2/M phase in different sesquiterpenoid dose groups were significantly different compared with that those in IL-22 experimental groups (P＜0.05). The cytokine levels of IL-22 experimental group were significantly different compared with that those in blank control group (P＜0.05). The content of TNF-α in ferulic acid and sesquiterpene groups was lower than that in IL-22 experimental group, and the difference was statistically significant (P＜0.05), but there was no significant difference between ferulic acid groups (P＞0.05), and the content of TNF-α in high-dose sesquiterpene group was lower than that in low-dose sesquiterpene dose group (P＜0.05). The IFN-γ content in ferulic acid and sesquiterpene groups was significantly different compared with that that in IL-22 group (P＜0.05). The IFN-γ content in ferulic acid and sesquiterpene high-dose groups was lower than that in medium-dose and low-dose groups (P＜0.05). The IFN-γ content in medium-dose group was lower than that in low-dose group (P＜0.05). The content of IL-23 in sesquiterpenoid dose groups were significantly different compared with that in the IL-22 experimental group (P＜0.05). The content of IL-23 in sesquiterpenoid high-dose group was lower than that in sesquiterpenoid medium-dose group and low-dose group (P＜0.05). There was no significant difference in IL-4 content among treatment groups (P＞0.05). Conclusion Sesquiterpenoids can inhibit IL-22-induced abnormal proliferation of HaCaT cells and regulate cell cycle, which is the important component in the treatment of psoriasis.

Key words： Psoriasis Ferula Sinkiang Cell proliferation Sesquiterpenoids

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	SUN Shifang1 ZHAN Mingfeng1 SHEN Xiaofeng1 YU Junfeng2

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SUN Shifang1 ZHAN Mingfeng1 SHEN Xiaofeng1 YU Junfeng2. Study on the effect of Ferula Sinkiang root extract on the abnormal proliferation of keratinocytes induced by interleukin-22[J]. 中国医药导报, 2023, 20(14): 15-19.

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