|
|
|
| Analysis of effects of miR-143 regulation of TLR2 signaling pathway on ulcerative colitis based on bioinformatics |
| HAN Ling1 CHEN Qin2 XIE Changying3 HUANG Xueyun4 WU Na4 |
1.Aesthetic Medical School, Yichun University, Jiangxi Province, Yichun 336000, China;
2.Department of Anorectal Surgery, Kunming Municipal Hospital of Traditional Chinese Medicine, Yunnan Province, Kunming 650011, China;
3.Department of Anorectal Surgery, the Affiliated Hospital of Jiangxi University of Traditional Chinese Medicine, Jiangxi Province, Nanchang 330006, China; 4.the First Department of Digestive, the Affiliated Hospital of Jiangxi University of Traditional Chinese Medicine, Jiangxi Province, Nanchang 330006, China
|
|
|
|
Abstract Objective To explore the effects of miR-143 regulation of Toll-like receptor 2 (TLR2) signaling pathway on ulcerative colitis (UC) based on bioinformatics. Methods Gene data sets GSE75214, GSE53306, GSE48958 and miRNA data set GSE68306 were downloaded from GEO database for analysis. The target genes of the differential miRNAs meeting the criteria were predicted by miRDB software, and mapped with the screened differential genes, and the key miRNA in UC were selected for experiments. According to random number table method, 20 mice were divided into normal group and UC group, with 10 mice in each group. Sodium gluconosulfate was used for modeling. The pathological changes were observed by hematoxylin-eosin staining staining. The contents of tumor necrosis factor-α(TNF-α) were detected by ELISA, and the contents of TLR2 and interleukin-1β (IL-1β) in colon were detected by Western blot. The contents of miR-143, nuclear transcription factor-κB (NF-κB), and myeloid differentiation factor (MyD88) in colon tissue were determined by real-time quantitative PCR. Results There were 85 DEMs, including 34 DEMs were downregulated and 51 DEMS were up-regulated, and 19 DEMS (5 DEMS were highly expressed, and 14 DEMS were underexpressed), with miR-143 being the key miRNA. The results showed that compared with normal group, the serum TNF-α content in UC group was significantly increased, the expression of miR-143 in colon tissue was significantly decreased (P＜0.05), the protein expressions of TLR2 and IL-1β were significantly increased, and the mRNA contents of NF-κB and MyD88 were significantly increased (P＜0.05). Conclusion miR-143 is an important miRNA in ulcerative colitis, which may affect the development of UC through regulation of TLR2 pathway.
|
|
|
|
|
|
[1] Jeong DY，Kim S，Son MJ，et al. Induction and maintenance treatment of inflammatory bowel disease：A comprehensive review [J]. Autoimmun Rev，2019，18（5）：439-454.
[2] 梁妍，丛春莉，杜春，等.溃疡性结肠炎合并阑尾周围炎的研究进展[J].现代消化及介入诊疗，2022，27（5）：648- 652.
[3] 陈茜茜，朱海勇.溃疡性结肠炎患者活动期肠道菌群及粪便炎症标志物检测及其临床意义[J].中国现代医生，2021，59（34）：16-19.
[4] 李学锋，彭霞，周明欢.我国炎症性肠病流行病学研究进展[J].现代消化及介入诊疗，2020，25（9）：1265-1267.
[5] 杨彬.健脾止泻汤治疗溃疡性结肠炎的临床效果[J].中国医学创新，2021，18（32）：92-95.
[6] 代春燕，朱宇，孔欣，等.韩捷教授运用祛瘀扶正法治疗激素抵抗型溃疡性结肠炎经验[J].中国医药科学，2022， 12（1）：63-66.
[7] Nakov R. New markers in ulcerative colitis [J]. Clin Chim Acta，2019，497：141-146.
[8] Hill M，Tran N. miRNA interplay：mechanisms and conseque- nces in cancer [J]. Dis Model Mech，2021，14（4）：dmm047662.
[9] James JP，Riis LB，Malham M，et al. MicroRNA Biomarkers in IBD-Differential Diagnosis and Prediction of Colitis- Associated Cancer [J]. Int J Mol Sci，2020，21（21）：7893.
[10] Chen L，Heikkinen L，Wang C，et al. Trends in the development of miRNA bioinformatics tools [J]. Brief Bioinform，2019， 20（5）：1836-1852.
[11] Zhou J，Liu J，Gao Y，et al. miRNA-Based Potential Biomarkers and New Molecular Insights in Ulcerative Colitis [J]. Front Pharmacol，2021，12：707776.
[12] Barrett T，Wilhite SE，Ledoux P，et al. NCBI GEO：archive for functional genomics data sets—update [J]. Nucleic Acids Res，2013，41（Database issue）：D991-D995.
[13] Wong N，Wang X. miRDB：an online resource for microRNA target prediction and functional annotations [J]. Nucleic Acids Res，2015，43（Database issue）：D146-D152.
[14] 刘素萍，王海燕，赵海梅，等.左金丸对DSS诱导溃疡性结肠炎小鼠滤泡辅助性T细胞的调控作用[J].中药新药与临床药理，2021，32（3）：301-306.
[15] Li JM，Zhao RH，Li ST，et al. Down-regulation of fecal miR-143 and miR-145 as potential markers for colorectal cancer [J]. Saudi Med J，2012，33（1）：24-29.
[16] Akao Y，Nakagawa Y，Naoe T. MicroRNA-143 and -145 in colon cancer [J]. DNA Cell Biol，2007，26（5）：311-320.
[17] Gomes SE，Pereira DM，Roma-Rodrigues C，et al. Conve- rgence of miR-143 overexpression，oxidative stress and cell death in HCT116 human colon cancer cells [J]. PLoS One，2018，13（1）：e0191607.
[18] Guo H，Chen Y，Hu X，et al. The regulation of Toll-like receptor 2 by miR-143 suppresses the invasion and migration of a subset of human colorectal carcinoma cells [J]. Mol Cancer，2013，12：77.
[19] Liu X，Gong J，Xu B. miR-143 down-regulates TLR2 expression in hepatoma cells and inhibits hepatoma cell proliferation and invasion [J]. Int J Clin Exp Pathol，2015，8（10）：12738-12747.
[20] Pekow JR，Dougherty U，Mustafi R，et al. miR-143 and miR-145 are downregulated in ulcerative colitis：putative regulators of inflammation and proto-oncogenes [J]. Inflamm Bowel Dis，2012，18（1）：94-100.
[21] Fitzgerald KA，Kagan JC. Toll-like Receptors and the Control of Immunity [J]. Cell，2020，180（6）：1044-1066.
[22] Flórez-álvarez L，Ruiz-Perez L，Taborda N，et al. Toll-like receptors as a therapeutic target in cancer，infections and inflammatory diseases [J]. Immunotherapy，2020，12（5）：311- 322.
[23] Vijay K. Toll-like receptors in immunity and inflammatory diseases：Past，present，and future [J]. Int Immunopharmacol，2018，59：391-412.
[24] 夏宇，李健，高鸿亮.TLR2、TLR4和NOD2/CARD15基因多态性与溃疡性结肠炎相关性的meta分析[J].胃肠病学，2021，26（2）：82-90. |
|
|
|
