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| Mechanism study of sevoflurane on proliferation and apoptosis of colorectal cancer cells by regulating miR-221 |
| XU Jie MIAO Xiaolei WANG Hui ZHANG Jian |
Department of Anesthesiology, Beijing Chaoyang Hospital, Capital Medical University, Beijing 100020, China
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Abstract Objective To investigate the effect of sevoflurane on proliferation and apoptosis of colorectal cancer cells through regulation of miR-221. Methods HCT116 cells were treated with low (1.7%), medium (3.4%) and high (5.1%) doses of sevoflurane for 6 h, respectively, and cells were stimulated with high doses of sevoflurane after transfection as required. CCK-8, clone formation assay and flow cytometry were used to detect cell proliferation and apoptosis. Results The cell OD values at all time points in the medium and high dose groups were lower than those in the low dose group; the cell OD values at all time points in the high dose group were lower than those in the medium dose group (P＜0.05). The clone formation rates of the low, medium, and high dose sevoflurane-treated groups were lower than those of the control group; the cell clone formation rates of the medium and high dose groups were lower than those of the low dose group; the cell clone formation rates of the high dose group were lower than those of the medium dose group (P＜0.05). The apoptosis rate in the low, medium, and high dose sevoflurane-treated groups were higher than that in the control group; the apoptosis rate in the medium and high dose groups were higher than that in the low dose group, and the apoptosis rate in the high dose group was higher than that in the medium dose group (P＜0.05). The miR-221 expression levels in HCT116 cells in the low, medium, and high dose sevoflurane-treated groups were lower than those in the control group, miR-221 expression levels in HCT116 cells in the medium and high dose groups were lower than those in the low dose group, and miR-221 expression levels in HCT116 cells in the high dose group were lower than those in the medium dose group (P＜0.05). The cell OD values at all time points in the anti-miR-221 group were lower than those in the anti-miR-NC group (P＜0.01). The clone formation rate of HCT116 cells in the anti-miR-221 group was lower than that in the anti-miR-NC group, and the apoptosis rate was higher than that in the anti-miR-NC group (P＜0.01). The cell OD values were higher in the miR-221 group than in the miR-NC group at each time point (P＜0.05). The cell clone formation rate in the miR-221 group was higher than that in the miR-NC group, and the apoptosis rate was lower than that in the miR-NC group (P＜0.01). The cell OD values of miR-221+sevoflurane were higher than those of miR-NC+sevoflurane group at each time point (P＜0.01). The cell clone formation rate of miR-221+sevoflurane group was higher than that of miR-NC+sevoflurane group and the apoptosis rate was lower than that of miR-NC+sevoflurane group (P＜0.05). Sevoflurane inhibits colorectal cancer cell proliferation and promotes apoptosis by suppressing miR-221 expression. Conclusion Sevoflurane inhibits the proliferation and promotes apoptosis of CRC cells by down-regulating miR-221.
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