儿童急性淋巴细胞白血病中Wnt通路抑制因子-1基因甲基化研究

doi:10.20047/j.issn1673-7210.2023.11.27

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Abstract Objective To investigate the expression of Wnt inhibitory factor-1 (Wif-1) and its methylation status in childhood acute lymphoblastic leukemia (ALL). Methods A total of 43 newly diagnosed children with ALL from June 2017 to February 2022 at the Affiliated Hospital of Xuzhou Medical University were selected as the experimental group, 20 bone marrow transplant donors were selected as the control group. 3-5 ml of bone marrow blood from each of the experimental group and control group were collected, Wif-1 mRNA expression was detected by quantitative real-time fluorescence polymerase chain reaction; Wif-1 protein expression was detected by Western blot; Wif-1 gene methylation status was detected by methylation specific polymerase chain reaction. Results The relative expression of Wif-1 mRNA and protein in the experimental group was lower than that in the control group, and the difference was statistically significant (P＜0.05); the positive rate of Wif-1 gene methylation in the experimental group was higher than that in the control group, the difference was statistically significant (P＜0.05). Conclusion Wif-1 gene hypermethylation exists in childhood ALL.

Key words： Acute lymphoblastic leukemia Wnt inhibitory factor-1 Methylation Wnt signaling pathway

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	HAN Wei WANG Wenpeng YAN Jing LI Xuan GAO Jizhao

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HAN Wei WANG Wenpeng YAN Jing LI Xuan GAO Jizhao. Methylation of Wnt inhibitory factor-1 gene in childhood acute lymphoblastic leukemia[J]. 中国医药导报, 2023, 20(11): 119-122.

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https://www.yiyaodaobao.com.cn/EN/10.20047/j.issn1673-7210.2023.11.27 OR https://www.yiyaodaobao.com.cn/EN/Y2023/V20/I11/119

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