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| Study on anti-anxiety effect of Hemerocallis citrina Borani and its mechanism |
| WANG Shaonan1 ZHANG Yunqi1 JING Songsong1 ZHENG Yuguang2 SUN Zhiyong3 |
1.College of Pharmacy, Heibei University of Chinese Medicine, Hebei Province, Shijiazhuang 050200, China;
2.Hebei Chemical and Pharmaceutical College, Hebei Province, Shijiazhuang 050200, China;
3.the Fourth Hospital of Shijiazhuang, Hebei Province, Shijiazhuang 050200, China
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Abstract Objective To determine the anti-anxiety effect of Hemerocallis citrina Borani（XCH） and its mechanism. Methods Sixty male ICR mice were divided into 6 groups by random number table method, including hemerocallis low-dose group (1 g/kg), medium-dose group (4 g/kg), high-dose group (8 g/kg), blank group (normal saline), model group and diazepam positive control group (2 mg/kg), with 10 mice in each group. Except the blank group, the other groups were prepared by chronic restraint stress. Mice of each group underwent elevated cross maze, open field and light and dark box experiments. The levels of dopamine (DA), 5-hydroxytryptamine (5-HT), gamma-aminobutyric acid (GABA) and glutamic acid (Glu) in the brain of mice were determined by enzyme-linked immunosorbent assay. Results Compared with blank group, the number of times of entering the central area decreased (P＜0.05), the time of staying in the central area shortened (P＜0.01), and the percentage of open arm time (OT%) and the percentage of open arm times (OE%) decreased in model group (P＜0.05); the times of entering the open box were reduced, and the activity time of the open box was shortened (P＜0.05). Compared with model group, the number of times of entering the central region in medium and high dose groups increased (P＜0.01); the residence time in the central region of low and medium dose groups was prolonged (P＜0.05). OT% of low, medium and high dose groups was increased (P＜0.05 or P＜0.01); the OE% of medium and high dose groups was increased (P＜0.05 or P＜0.01); the number of mice entering the open chamber increased in the medium dose group (P＜0.05); the open chamber activity time of mice in medium and high dose groups was prolonged (P＜0.01). Compared with blank group, the content of GABA in brain tissue of model group was significantly decreased, while the contents of GLU, 5-HT and DA were significantly increased (P＜0.05). Compared with the model group, the GABA level in the brain tissues of the low, medium and high dose groups was increased (P＜0.05 or P＜0.01). The 5-HT and DA level in the brain tissues of the medium dose group was reduced (P＜0.05); the GLU and DA level in the brain tissues of the high dose group was decreased (P＜0.05). Conclusion XCH shows obvious anti-anxiety effects, which mechanism may be related to increasing the level of GABA and decreasing the level of DA and 5-HT in the brain tissues.
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