|
|
Analysis on the quality of loquat leaves from Tangxi |
MA Zhelong1 WU Zengyan1 JIANG Fusheng2 DING Zhishan3 |
1.Department of TCM Pharmacy, the Fifth People′s Hospital of Yuhang District in Hangzhou City, Zhejiang Province, Hangzhou 311100, China;
2.College of Life Science, Zhejiang Chinese Medical University, Zhejiang Province, Hangzhou 310053, China;
3.College of Medical Technology, Zhejiang Chinese Medical University, Zhejiang Province, Hangzhou 310053, China |
|
|
Abstract Objective To evaluate the quality of different varieties of loquat leaves from Tangxi. Methods Reference to the 2015 edition of Chinese Pharmacopoeia, the water content, total ash content and extract content of different varieties of loquat leaves were measured by oven drying method, ignition method and hot-dip method. The oleanolic acid and ursolic acid were detected useing Kromasil C18 (250 mm×4.6 mm, 5 μm) column, with mobile phase being acetonitrile-methanol-0.5% ammonium acetate (67∶12∶21, v/v/v), column temperature being at 25℃, flow velocity being 1.0 mL/min, and detection wavelength being at 210 nm. The quercetin and kaempferol were detected by Kromasil C18(250 mm×4.6 mm,5 μm)column, with mobile phase being methanol-0.4% phosphoric acid (50∶50, v/v), column temperature being at 30℃, flow velocity being 1.0 mL/min, and detection wavelength being at 360 nm. Results The range of water content, total ash content, and extract content of different varieties of loquat leaves were 7.87%-9.45%, 5.14%-6.22% and 25.41%-29.68%, respectively, the range of total content of oleanolic acid and ursolic acid was 1.04%-1.25%, all in line with the 2015 edition of Chinese Pharmacopoeia. In addition, the range of total content of quercetin and kaempferol in loquat leaves was 0.17%-0.29%. Conclusion The different varieties of loquat leaves in Tangxi all have good quality and deserve further development and utilization.
|
|
|
|
|
[1] 国家药典委员会.中国药典[S].北京:中国医药科技出版社,2015.
[2] 柯仲成,朱志平,徐志远,等.枇杷叶的研究进展[J].内蒙古医科大学学报,2016,38(1):84-87.
[3] 吕寒,陈剑,李维林,等.枇杷叶中三萜类化学成分的研究[J].中药材,2008,31(9):1351-1354.
[4] 陈欢,陈光,任红梅,等.枇杷叶中三萜类成分的研究[J].北京化工大学学报:自然科学版,2012,34(3):40-45.
[5] 吕寒,李维林,裴咏萍,等.枇杷叶中黄酮类化学成分的HPLC-MSn分析[J].现代中药研究与实践,2009,22(6):56-58.
[6] 黄素华,陈秋妹,陈瑞红,等.枇杷叶多糖的分离纯化及组分鉴定[J].食品研究与开发,2017,38(4):42-48.
[7] 钟永康.枇杷叶化学成分及提取分离方法的研究进展[J].今日药学,2017,27(9):646-648.
[8] 葛金芳,李俊,金涌,等.枇杷叶三萜酸的镇咳祛痰平喘作用[J].安徽医科大学学报,2006,41(4):413-416.
[9] 葛金芳,李俊,姚宏伟,等.枇杷叶三萜酸的抗炎作用[J].安徽医科大学学报,2007,42(2):174-178.
[10] 王立为,刘新民,余世春,等.枇杷叶抗炎和止咳作用研究[J].中草药,2004,35(2):174-176.
[11] 宋星,王婷婷,杨晓丹,等.枇杷叶中含科罗索酸提取物的分离纯化及降血糖活性研究[J].中国新药杂志,2017,26(2):214-219.
[12] 洪雷.枇杷叶多糖分离纯化及生物活性研究[D].福州:福建农林大学,2008:28-43.
[13] 朱敏芳,张捷,冯丽,等.枇杷叶提取物对中性粒细胞弹性蛋白酶的抑制作用[J].中成药,2017,39(1):177-180.
[14] 郭磊,张轩斌.枇杷叶三萜酸对慢性支气管炎大鼠的疗效及其机制[J].中药材,2015,38(10):2166-2168.
[15] 吴月娴,吕寒,简暾昱,等.不同类型枇杷叶三萜酸类成分含量及降糖活性的比较[J].植物资源与环境学报,2017, 26(4):101-103.
[16] 谭冰心,黄仪有,彭光天,等.不同产地枇杷叶粗提物抑制磷酸二酯酶4活性研究[J].药物评价研究,2017,40(6):769-772.
[17] 吕寒,滕杰晖,陈剑,等.枇杷叶总黄酮的纯化工艺及抗氧化活性研究[J].中国现代应用药学,2014,31(1):40-44.
[18] 任冰如,陈剑,吕寒,等.HPLC测定红凤菜总黄酮含量[J].中国野生植物资源,2016,35(1):28-30.
[19] 韦媛媛,徐峰,陈侠,等.桑白皮总黄酮的镇咳祛痰作用[J].沈阳药科大学学报,2009,26(8):644-647.
[20] 潘华新,梁瑞燕,曹柳英,等.柚总黄酮祛痰、止咳作用研究[J].中药材,2007,30(1):80-82.
[21] 阿迪拉木·阿依甫江,麦合苏木·艾克木,古孜力努尔·依马木,等.维药铁线蕨总黄酮抗炎平喘作用研究[J].新疆医科大学学报,2016,39(1):17-20. |
|
|
|