Expression and clinical significance of lncRNA MNX1-AS1 and miR-218-5p in non-small cell lung cancer tissues
WANG Qiyuan1 LI Chengyin1 ZHU Jing1 ZOU Yinshui1 ZHAO Yaying1 GUO Guanghui2
1.Department of Oncology，Hubei Provincial Hospital of Traditional Chinese medicine, Hubei Province, Wuhan 430061, China;
2.Department of Respiratory, Hubei Provincial Hospital of Traditional Chinese Medicine, Hubei Province, Wuhan 430061, China
Abstract Objective To study the expression and clinical significance of long non-coding RNA (LncRNA) MNX1-AS1 and microRNA (miR)-218-5p in non-small cell lung cancer (NSCLC) tissues. Methods A total of 120 NSCLC patients diagnosed and treated in Hubei Provincial Hospital of Traditional Chinese Medicine from January 2015 to January 2017 were selected. The expression of LncRNA MNX1-AS1 and miR-218-5p were detected by fluorescence quantitative PCR from NSCLC tissues and paracancer tissues. The correlation between LncRNA MNX1-AS1 and miR-218-5p in NSCLC tissues and its clinicopathologic features were analyzed. The mean of LncRNA MNX1-AS1 and miR-218-5p relative expression levels (2.321、1.213) were divided into high and low expression groups to analyze their effects on survival and prognosis of patients with NSCLC. Results The expression of LncRNA MNX1-AS1 and miR-218-5p in NSCLC tissues was higher than that in paracancer tissues (P＜0.05). LncRNA MNX1-AS1 was negatively correlated with miR-218-5p (r=-0.561, P＜0.01). The expression of LncRNA MNX1-AS1 and miR-218-5p in patients with different lymph node metastases and tumor stages were statistically significant (P＜0.05). The survival curve of LncRNA MNX1-AS1 high-expression group was lower than that of LncRNA MNX1-AS1 low-expression group, and the survival curve of miR-218-5p low-expression group was lower than that of miR-218-5p high-expression group (P＜0.05). Conclusion The expression of LncRNA MNX1-AS1 increased and miR-218-5p decreased in NSCLC tissues, both of which are associated with poor prognosis of patients and may serve as prognostic evaluation indicators.
WANG Qiyuan1 LI Chengyin1 ZHU Jing1 ZOU Yinshui1 ZHAO Yaying1 GUO Guanghui2. Expression and clinical significance of lncRNA MNX1-AS1 and miR-218-5p in non-small cell lung cancer tissues[J]. 中国医药导报, 2023, 20(8): 99-102,110.
[1] Bade BC，Dela Cruz CS. Lung Cancer 2020：Epidemiology，Etiology，and Prevention [J]. Clin Chest Med，2020，41（1）：1-24.
[2] Wang J，Zhu S，Meng N，et al. ncRNA-Encoded Peptides or Proteins and Cancer [J]. Mol Ther，2019，27（10）：1718- 1725.
[3] Liu X，Yang Q，Yan J，et al. LncRNA MNX1-AS1 promotes the progression of cervical cancer through activating MAPK pathway [J]. J Cell Biochem，2019，120（3）：4268-4277.
[4] Ma B，Ren G，Xu J，et al. LncRNA MNX1-AS1 Contributes to Laryngeal Squamous Cell Carcinoma Growth and Migration by Regulating mir-744-5p/bcl9/β-Catenin Axis [J]. Cell Transplant，2021，30（5）：9636-9642.
[5] Peng P，Chen T，Wang Q，et al. Decreased miR-218-5p Levels as a Serum Biomarker in Bone Metastasis of Prostate Cancer [J]. Oncol Res Treat，2019，42（4）：165-185.
[6] Li Y，Shi B，Dong F，et al. LncRNA KCNQ1OT1 facilitates the progression of bladder cancer by targeting MiR-218- 5p/HS3ST3B1 [J]. Cancer Gene Ther，2021，28（3/4）：212- 220.
[7] 任占良，张泳，任小朋，等.非小细胞肺癌手术单孔胸腔镜淋巴结清除术应用研究[J].中华肿瘤防治杂志，2021，28（5）：365-368.
[8] Hui Z，Men Y，Hu C，et al. Effect of Postoperative Radiotherapy for Patients With pⅢA-N2 Non-Small Cell Lung Cancer After Complete Resection and Adjuvant Chemotherapy：The Phase 3 PORT-C Randomized Clinical Trial [J]. JAMA Oncol，2021，7（8）：1178-1185.
[9] 夏雯洁，张雅婷，涂琳琳，等.长链非编码RNA作为自身免疫病生物标志物的研究进展[J].现代免疫学，2022，42（1）：64-71.
[10] 汪贝贝，张文波，蒋鹏程.长链非编码RNA参与调控细胞程序性死亡的研究进展[J].中国比较医学杂志，2022， 32（5）：147-154.
[11] Zhou B，Yang H，Yang C，et al. Translation of noncoding RNAs and cancer [J]. Cancer Lett，2021，497（28）：89- 99.
[12] Liu H，Han L，Liu Z，et al. Long noncoding RNA MNX1- AS1 contributes to lung cancer progression through the miR-527/BRF2 pathway [J]. J Cell Physiol，2019，234（8）：13843-13850.
[13] 张维，张珂，蒋力，等.lncRNA MNX1-AS1在结直肠癌中的临床意义及功能研究[J].标记免疫分析与临床，2020， 27（5）：833-838.
[14] Wu Q，Luo X，Liu J，et al. MYC-Activated LncRNA Promotes the Progression of Colorectal Cancer by Stabilizing YB1 [J]. Cancer Res，2021，81（10）：2636-2650.
[15] Liang D，Tian C，Zhang X. lncRNA MNX1-AS1 promotes prostate cancer progression through regulating miR-2113/ MDM2 axis [J]. Mol Med Rep，2022，26（1）：231-240.
[16] Li J，Li Q，Li D，et al. Long Non-Coding RNA MNX1-AS1 Promotes Progression of Triple Negative Breast Cancer by Enhancing Phosphorylation of Stat3 [J]. Front Oncol，2020，10（6）：1108-1117.
[17] Hu S，Li Z，Lutz H，et al. Dermal exosomes containing miR- 218-5p promote hair regeneration by regulating β-catenin signaling [J]. Sci Adv，2020，6（30）：1685-1692.
[18] Yang Y，Zhao F，Yuan Z，et al. Inhibition of miR-218-5p reduces myocardial ischemia-reperfusion injury in a Sprague-Dawley rat model by reducing oxidative stress and inflammation through MEF2C/NF-κB pathway [J]. Int Immunopharmacol，2021，101（Pt B）：1082-1099.
[19] 朱海龙，朱旭友.结直肠腺瘤中的miR-218-5p表达研究[J].中国当代医药，2021，28（35）：91-94.
[20] 杜正平，李海龙，刘春萍，等.miR-218-5p在胃癌细胞和胃癌组织中表达的临床意义及生物信息学分析[J]. 检验医学与临床，2020，17（7）：876-880.
[21] Wang J，Shu H，Xu C，et al. LncRNA DGCR5 promotes non- small cell lung cancer progression via sponging miR-218- 5p [J]. Eur Rev Med Pharmacol Sci，2019，23（22）：9947- 9954.
[22] Wang A，Dai H，Gong Y，et al. ANLN-induced EZH2 upregulation promotes pancreatic cancer progression by mediating miR-218-5p/LASP1 signaling axis [J]. J Exp Clin Cancer Res，2019，38（1）：347-353.
[23] Meng X，Zhang Y，Hu Y，et al. LncRNA CCAT1 sponges miR-218-5p to promote EMT，cellular migration and invasion of retinoblastoma by targeting MTF2 [J]. Cell Signal，2021，86（5）：1100-1112.
[24] Wang H，Zhan M，Xu S，et al. miR-218-5p restores sensitivity to gemcitabine through PRKCE/MDR1 axis in gallbladder cancer [J]. Cell Death Dis，2017，8（5）：2770- 2781.
[25] 曹军营，崔素娟，刘高峰，等.lncRNA MNX1-AS1通过调控miR-218-5p影响非小细胞肺癌细胞的增殖、凋亡及迁移[J].中南医学科学杂志，2022，50（3）：336- 340.
