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| Study of effect in Fructus Corni extract on radiation damage of hematopoietic system in mice |
| LI Wenxuan1 WANG Yue2 WANG Xinyue1 HUO Qidong1 DONG Yinping1 TANG Sheng’an2 DONG Hui1 LI Deguan1 |
1.Tianjin Key Laboratory of Radiation Medicine and Molecular Nuclear, Medicine Institute of Radiation Medicine, Chinese Academy of Medical Science, Tianjin 300192, China;
2.Tianjin Key Laboratory on Technologies Enabling Development of Clinical Therapeutics and Diagnostics (Theranostics), Tianjin Medical University, Tianjin 300070, China
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Abstract Objective To study the protective effect of Fructus Corni extract on hematopoietic system damage induced by radiation in mice. Methods Sixteen male SPF level C57BL/6J mice aged 6 to 8 weeks, weighing 20 to 22 g, were selected. One of them was randomly selected to isolate the femur and culture its bone marrow cells in vitro. Cell control group, cell irradiation group, 0.001 mg/ml Fructus Corni group, 0.01 mg/ml Fructus Corni group were set. The cell control group was cultured normally, the cell irradiation group was irradiated (1 Gy), the 0.001 mg/ml Fructus Corni group and 0.01 mg/ml Fructus Corni group were irradiated with corresponding concentrations of Fructus Corni extract for 30 min, and the cell vitality of the four groups was observed 18 h later, and the concentration of Fructus Corni extract was selected according to the cell vitality. The apoptotic degree and reactive oxygen species levels in cell control group, cell irradiation group, and 0.001 mg/ml Fructus Corni group were compared. The remaining 15 mice were divided into control group, irradiation group, and Fructus Corni group with five mice in each group by randomized block method. The irradiation group and Fructus Corni group were given 2 Gy total body irradiation, the Fructus Corni group was given 0.2 ml (400 mg/kg) DMSO solution of Fructus Corni extract 1 h before irradiation, and the control group and the irradiation group were given the same amount of normal saline containing 8%DMSO. After seven days of administration, routine blood and the number of bone mononuclear nucleated cell (BMNC), hematopoietic progenitor cell (HPC), hematopoietic stem cell (HSC), reactive oxygen species, and DNA (γH2AX) damage were detected in peripheral blood of the three groups. Results The cell vitality in the cell irradiation group was lower than that in the cell control group, and the proportion of apoptotic cells and the level of reactive oxygen species were higher than those in the cell control group (P＜0.05). There was no significant difference in cell viability between 0.01 mg/ml Fructus Corni group and cell irradiation group (P＞0.05). The cell vitality of 0.001 mg/ml Fructus Corni group was higher than that of cell irradiation group, and the percentage of apoptotic cells and reactive oxygen species level were lower than those of cell irradiation group (P＜0.05). The levels of white blood cell, red blood cell, platelet, HPC, and HSC in irradiation group were lower than those in control group (P＜0.05). There were no significant differences in BMNC level among the three groups (P＞0.05). The levels of red blood cell and HPC in Fructus Corni group were higher than those in irradiation group (P＜0.05), but there were no significant differences in the levels of white blood cell, platelet, and HSC between the two groups (P＞0.05). There were no significant differences in reactive oxygen species in BMNC, HPC, and HSC of three groups, and γH2AX level in BMNC of three groups (P＞0.05). γH2AX levels in HPC and HSC of irradiation group were higher than those in control group, and γH2AX levels in HPC and HSC of Fructus Corni group were lower than those in irradiation group (P＜0.05). Conclusion Fructus Corni extract can prevent radiation damage of hematopoietic system.
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