|
|
|
| Protective effect of endothelium acupuncture preconditioning on neural function in rats with cerebral ischemia reperfusion |
| JIANG Wei1 LIU Huihui2▲ XIAO Xiaoyue3 CHU Junbo1 LIU Xu1 HONG Shenghan1 |
1.Teaching and Research Section of Massage, the First Affiliated Hospital of Heilongjiang University of Chinese Medicine, Heilongjiang Province, Harbin 150036, China;
2.Department of Acupuncture and Moxibustion, the Second Affiliated Hospital of Heilongjiang University of Chinese Medicine, Heilongjiang Province, Harbin 150040, China;
3.College of Animal Medicine, the Northeast Agricultural University, Heilongjiang Province, Harbin 150006, China
|
|
|
|
Abstract Abstract To study the protective effect of intracutaneous acupuncture preconditioning on neural function in rats with cerebral ischemia/reperfusion (CI/R). Method A total of 120 SD male rats aged eight weeks and weighing (230±10)g were divided into four groups (sham operation group, model group, electro-acupuncture group and endothelium acupuncture group), with 30 rats in each group. In the endothelium acupuncture group and electro-acupuncture group, the rats were pretreated with endothelium acupuncture and electro-acupuncture respectively, and then the middle cerebral artery occlusion (MCAO) reperfusion model of rats was prepared. The model group only accepts modeling treatment. In the sham operation group, only open neck surgery was performed, but no ligation was performed. Neurological impairment was analyzed by modified neurological deficit score (mNSS), and the levels of inflammatory cytokines interleukin(IL)-1β and IL-6 were measured by enzyme-linked immunosorbent assay. The expression levels of Caspase-3, Bax and BCL-2 were detected by Western blot. Results mNSS score in the model group was higher than that in the sham operation group (P＜0.05), mNSS scores in the electro-acupuncture group and endothelium acupuncture group were lower than that in the model group, and the differences were statistically significant (P＜0.05). The contents of IL-1β and IL-6 in model group were higher than those in sham operation group, and the differences were statistically significant (P＜0.05). The contents of IL-1β and IL-6 in electro-acupuncture group and endothelium acupuncture group were lower than those in model group, and the differences were statistically significant (P＜0.05). The protein levels of Caspase-3 and Bax in model group were higher than those in sham operation group, and the protein levels of BCL-2 were lower than those in sham operation group, and the differences were statistically significant (P＜0.05). The protein levels of Caspase-3 and Bax in electro-acupuncture group and endothelium acupuncture group were lower than those in model group, and the protein levels of BCL-2 were higher than those in model group, and the differences were statistically significant (P＜0.05). Conclusion Endothelium acupuncture pretreatment could significantly reduce the inflammatory factors of CI/R rats, inhibit neuronal apoptosis, and reduce the brain nerve function damage of CI/R rats. Moreover, intradermal needle pretreatment also has the advantages of convenience.
|
|
|
|
|
|
[1] Liao S，Apaijai N，Chattipakorn N. The possible roles of necroptosis during cerebral ischemia and ischemia/reperfusion injury [J]. Arch Biochem Biophys，2020，695：108629.
[2] Yang J，Chen M，Cao RY，et al. The role of circular RNAs in cerebral ischemic diseases：ischemic stroke and cerebral ischemia/reperfusion injury [J]. Adv Exp Med Biol，2018，1087：309-325.
[3] 丁元庆，陈哲，唐赛雪.《金匮要略》对中风研究的贡献与影响[J].山东中医药大学学报，2020，44（5）：5.
[4] Huang Y，Tao W，Yang S. Autophagy：novel insights into therapeutic target of electroacupuncture against cerebral ischemia/reperfusion injury [J]. Neural Regen Res，2019，14（6）：954-961.
[5] Zhang Y. Mechanism of neuroprotective effect of stevioside on cerebral ischemia-reperfusion injury via PPAR-γ activation [J]. Immunopharm Immunot，2021，43（6）：704-712.
[6] 王永炎，范逸品，张华敏.从五运六气学说认识疫病流行的经验积累—读《黄帝内经·素问》遗篇《刺法论篇》《本病论篇》有感[J].北京中医药大学学报，2020，43（6）：4.
[7] 王浩然，王爱芸，沈庆思.齐鲁医家杨继洲《针灸大成》学术思想浅析[J].辽宁中医杂志，2016，43（6）：3.
[8] Kishimoto M，Suenaga J，Takase H. Oxidative stress-responsive apoptosis inducing protein （ORAIP） plays a critical role in cerebral ischemia/reperfusion injury [J]. Sci Rep，2019，9（1）：1-12.
[9] Xu H，Mu S，Qin W. Microglia TREM2 is required for electroacupuncture to attenuate neuroinflammation in focal cerebral ischemia/reperfusion rats [J]. Biochem Bioph Res Co，2018，503（4）：3225-3234.
[10] Khamchai S，Chumboatong W，Hata J. Morin protects the blood-brain barrier integrity against cerebral ischemia reperfusion through anti-inflammatory actions in rats [J]. Sci Rep，2020，10（1）：1-13.
[11] Wang J，Mao J，Wang R. Kaempferol protects against cerebral ischemia reperfusion injury through intervening oxidative and inflammatory stress induced apoptosis [J]. Front Pharmacol，2020，11：424.
[12] Wu L，Xiong X，Wu X. Targeting oxidative stress and inflammation to prevent ischemia-reperfusion injury [J]. Front Mol Neuro，2020，13：28.
[13] Diaz-Ca?觡estro C，Reiner M，Bonetti N. AP-1 transcription factor JunD regulates ischemia/reperfusion brain damage via IL-1β [J]. Stroke，2019，50（2）：469-477.
[14] Dai M，Wu L，Yu K. D-Carvone inhibit cerebral ischemia/ reperfusion induced inflammatory response TLR4/NLRP3 signaling pathway [J]. Biomed Pharmacother，2020，132：110870.
[15] Lee B，Lim C，Lim S. Baicalin administered orally after ischemia/reperfusion alleviated brain injury in mice by inhibiting inflammation and edema [J]. Nat Prod Commun，2019，14（5）：1934578.
[16] Wang L，Zhao H，Zhai Z. Protective effect and mechanism of ginsenoside Rg1 in cerebral ischaemia-reperfusion injury in mice [J]. Biomed Pharmacother，2018，99：876-882.
[17] Li S，Luo L，He Y，et al. Dental pulp stem cell‐derived exosomes alleviate cerebral ischaemia‐reperfusion injury through suppressing inflammatory response [J]. Cell Proliferat，2021，54（8）：e13093.
[18] Foroshani M，Sobhani Z，Mohammadi M. Fullerenol nanoparticles decrease blood-brain barrier interruption and brain edema during cerebral ischemia-reperfusion injury probably by reduction of interleukin-6 and matrix metalloproteinase-9 transcription [J]. J Stroke Cerebrovasc，2018，27（11）：3053-3065.
[19] 刘丹妮，周君，黄夏荣.电针“水沟”“百会”穴对脑缺血再灌注损伤大鼠海马神经元自噬的影响[J].针刺研究，2022，47（6）：491-496.
[20] He R，Jiang Y，Shi Y. Curcumin-laden exosomes target ischemic brain tissue and alleviate cerebral ischemia-reperfusion injury by inhibiting ROS-mediated mitochondrial apoptosis [J]. Mater Sci Eng C Mater Biol Appl，2020，117：111314.
[21] Zhang Y，Zhang Y. lncRNA ZFAS1 improves neuronal injury and inhibits inflammation，oxidative stress，and apoptosis by sponging miR-582 and upregulating NOS3 expression in cerebral ischemia/reperfusion injury [J]. Inflammation，2020，43（4）：1337-1350.
[22] Zhang Y，Zhang Y，Xiao Z. CFTR prevents neuronal apoptosis following cerebral ischemia reperfusion via regulating mitochondrial oxidative stress [J]. J Mol Med，2018，96（7）：611-620.
[23] Cai Y，Yang E，Yao X. FUNDC1-dependent mitophagy induced by tPA protects neurons against cerebral ischemia- reperfusion injury [J]. Redox Biol，2021，38：101792.
[24] Habib P，Stamm AS，Zeyen T. EPO regulates neuroprotective Transmembrane BAX Inhibitor-1 Motif-containing （TMBIM） family members GRINA and FAIM2 after cerebral ischemia-reperfusion injury [J]. Exp Neurol，2019， 320：112978.
[25] Wu M，Zhang H，Kai J. Rapamycin prevents cerebral stroke by modulating apoptosis and autophagy in penumbra in rats [J]. Ann Clin Transl Neur，2018，5（2）：138-146.
[26] Zhang L，Li S，Chen L. Cerebellar fastigial nucleus electrical stimulatin protects against cerebral ischemic damage by upregulating telomerase activity [J]. Restor Neurol Neuros，2019，37（2）：131-141. |
|
|
|
