茵陈蒿汤联合加味大柴胡汤对肝缺血再灌注损伤大鼠的影响

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Abstract Objective To observe the protective effect of Major Capillaries Decoction and Bubleurum Decoction on liver ischemia-reperfusion injury in rats. Methods Liver ischemia-reperfusion model rats were randomly divided into ischemia-reperfusion Chinese medicine group (TCM group, n = 75), ischemia-reperfusion control group (control group, n = 75), and sham operation group (sham group, n = 75). The contents of interleukin-1 (IL-1) in serum, intercellular adhesion molecule-1 (ICAM-1) and nuclear factor-kappa B (NF-κB) in rat liver homogenate tissue were measured by enzyme-linked immunosorbent assay (ELISA) and Western blot. Results After ischemia and reperfusion in three groups, serum IL-1 content gradually increased with the extension of time. In the same time after ischemia-reperfusion, the serum IL-1 content of TCM group was significantly lower than that of the control group, the differences were highly statistically significant (P ＜ 0.01). The expression of NF-κB and ICAM-1 in the liver homogenate tissue of TCM group and control group increased gradually with the extension of time, and the expression of TCM group was higher than that of control group at the same time point（P ＜ 0.05）. Conclusion Major Capillaries Decoction and Bubleurum Decoction can significantly alleviate liver ischemia-reperfusion injury in rats. It may be through inhibiting the activation of NF-κB and reducing the expression of ICAM-1 and IL-1 to play a protective role in ischemia-reperfusion injury.

Key words： Hepatic ischemia-reperfusion Interleukin-1 Intercellular adhesion molecule-1 Nuclear factor-κB

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	ZHANG Beiping1 ZHAI Shuping2 YUAN Wei2 YAN Yong2 ZHANG Xiaobing1 WANG Bailin2▲

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ZHANG Beiping1 ZHAI Shuping2 YUAN Wei2 YAN Yong2 ZHANG Xiaobing1 WANG Bailin2▲. The effect of Major Capillaries Decoction and Bubleurum Decoction on liver ischemia reperfusion injury in rats[J]. 中国医药导报, 2018, 15(30): 15-18.

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http://www.yiyaodaobao.com.cn/EN/ OR http://www.yiyaodaobao.com.cn/EN/Y2018/V15/I30/15

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