长链非编码RNA肿瘤蛋白翻译调节因子1-反义RNA1在神经胶质瘤细胞中的表达及对其增殖和侵袭的影响

doi:10.20047/j.issn1673-7210.2023.04.05

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Abstract Objective To investigate the effect of long non-coding RNA (lncRNA) tumor protein translation regulator 1-antisense RNA1 (TPT1-AS1) on the proliferation and invasion of glioma cells U251. Methods U251 cells were divided into blank control group, negative transfection group, and TPT1-AS1 overexpression group. The blank control group was not treated, and the other two groups were transfected with corresponding plasmids. After 48 hours of transfection, the expression level of TPT1-AS1 in U251 cells of each group was compared; MTT assay was used to determine the proliferation ability of U251 cells; flow cytometry was used to detect the apoptotic ability of U251 cells; Transwell chamber assay was used to detect the invasion ability of U251 cells; scratch test was used to detect the migration ability of U251 cells; the expression of apoptosis (Caspase-3, Bax, Bcl-2) and invasion (matrix metallo proteinases [MMP]-2, MMP-9) related proteins was detected by Western blot. Results There were no significant differences in TPT1-AS1 expression, cell survival rate, cell apoptosis rate, number of invasive cells, scratch healing rate, and protein expressions of Caspase-3, Bax, Bcl-2, MMP-2, and MMP-9 between negative control group and blank control group (P＞0.05). TPT1-AS1 expression level, apoptosis capacity, Caspase-3, and Bax protein expression in TPT1-AS1 overexpression group were higher than those in blank control group, and the differences were statistically significant (P＜0.05). The expression of Bcl-2 protein, cell survival rate, invasion, migration ability, and invasion-related protein in TPT1-AS1 overexpression group were lower than those in blank control group, with statistical significance (P＜0.05). Conclusion TPT1-AS1 has low expression in U251, and high expression of TPT1-AS1 can inhibit the proliferation and invasion of glioma cells.

Key words： Long non-loding RNA tumor protein translationally-controlled regulator 1-antisenseRNA1 Glioma Proliferation Invasion

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	LIU Zhenjie1 LI Xin1 LU Jing2 LIANG Liang1 HU Yaowen1 XIE Jing1 ZHAO Xingang3 YANG Jiankai4

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LIU Zhenjie1 LI Xin1 LU Jing2 LIANG Liang1 HU Yaowen1 XIE Jing1 ZHAO Xingang3 YANG Jiankai4. Expression of long non-coding RNA tumor protein translationally-controlled regulator 1-antisenseRNA1 in glioma and its effect on cell proliferation and invasion[J]. 中国医药导报, 2023, 20(4): 25-30.

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https://www.yiyaodaobao.com.cn/EN/10.20047/j.issn1673-7210.2023.04.05 OR https://www.yiyaodaobao.com.cn/EN/Y2023/V20/I4/25

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