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| The experimental study on promoting wound healing of keratinocyte growth factor bioactive peptides in diabetic rats |
| ZONG Xianlei1 CAO Chunyan1 SONG Guodong1 LAI Chenzhi1 YU Panxi1 JIN Xiaolei1 JIANG Duyin2,3 |
1.Sixteen Department of Plastic, Plastic Surgery Hospital, Chinese Academy Medical Sciences, Beijing 100144, China;
2.Department of Plastic and Burn Surger, the Second Hospital of Shandong University, Shandong Province, Ji′nan 250033, China;
3.Department of Emergency, the Second Hospital of Shandong University, Shandong Province, Ji′nan 250033, China |
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Abstract Objective To explore the promoting effect of keratinocyte growth factor (KGF) active peptide on wound healing in diabetic rats. Methods Twenty-four healthy female SD rats were selected, and injected intraperitoneally with streptozotocin to prepare a diabetic rat model. They were randomly divided into 4 groups: negative control group, KGF positive control group, No.1 KGF active short peptide group, and No.2 KGF active short peptide group, with 6 rats in each group. Round wounds of 2 cm in diameter were prepared on the back of the rats, and drugs of each group were injected locally on the wounds. Photographs were taken 14 days after injury, and the unhealed area of the wound was recorded to calculate the wound healing rate. The wound healing was observed by HE staining. Results The chronic wound model of diabetic rats was successfully established. At 14 days after injury, the wound healing rate of KGF positive control group, No.1 KGF active short peptide group and No.2 KGF active short peptide group was significantly higher than that of the negative control group, and the difference was highly statistically significant (P < 0.01). The wound healing rate of No.1 KGF active short peptide group and No.2 KGF active short peptide group was not significantly different from that of KGF positive control group (P > 0.05). In the negative control group, the whole skin of the wound was missing, and the skin attachments such as hair follicles, sweat glands and sebaceous glands were missing. The tissue structure was uniform and dense, no layer, and the inflammatory cell infiltration was obvious. In the other groups, the wounds were well formed, the deep tissue layers were clear, the structure was loose, the infiltrated inflammatory cells was lessen, and the skin attachments such as regenerated hair follicles, sweat glands and sebaceous glands were visible. Conclusion KGF active short peptide can promote wound healing and regeneration of skin attachment in diabetic rats.
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