防风中5-O-甲基维斯阿米醇苷对脂多糖诱导RAW264.7细胞炎症模型的抗炎作用研究

doi:10.20047/j.issn1673-7210.2023.02.03

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Abstract Objective To study the protective effect and mechanism of 4’-O-β-D-glucosyl-5-O-methylvisamminol in Radix Saposhnikoviae on inflammation of mice RAW264.7 cells induced by lipopolysaccharide (LPS). Methods RAW264.7 mononuclear macrophages were induced cellular inflammatory model by LPS. The proliferation effect of 4’-O-β-D-glucosyl-5-O-methylvisamminol with different concentrations(160, 80, 40, 20, 10 μmol/L) on mice RAW264.7 cells was detected by MTT assay. The experiment was divided into blank group, LPS group, positive control group (Indomethacin, 10 μmol/L), low (20 μmol/L), medium (40 μmol/L) and high (80 μmol/L) dose groups of 4’-O-β-D-glucosyl- 5-O-methylvisamminol. Blank medium was added to blank group and LPS group, and corresponding drugs were added to other dosing groups. LPS (10 μg/ml) was added after 2 h, the secretion of nitric oxide (NO) in supernatant of mice RAW264.7 was detected by Griess reagent method, the contents of prostaglandin E2 (PGE2) and tumor necrosis factor α (TNF-α) in the supernatant of mice RAW264.7 cells were determined by enzyme linked immunosorbent assay. The mRNA expressions of nuclear factor κB (NF-κB p65), interleukin-1β (IL-1β), inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) were detected by RT-PCR. Toll like receptor 4 (TLR4) and NF-κB p65 protein expressions were detected by Western blot. Results 4’-O-β-D- glucosyl-5-O- methylvisamminol (10-80 μmol/L) had no effect on RAW264.7 cell viability. Compared with LPS group, the contents of NO, PGE2 and TNF-α in mice RAW264.7 cells were decreased in low, medium and high doses of 5-O-methylvisamminol side groups (P＜0.01). The mRNA expressions of NF-kB p65, IL-1β, INOS and COX-2 and the protein expressions of TLR4 and NF-kB p65 were decreased (P＜0.01). Conclusion 4’-O-β-D-glucosyl-5-O- methylvisamminol in Radix Saposhnikoviae inhibit the inflammation of RAW264. 7 cells induced by LPS has anti- inflammatory effect.

Key words： Saposhnikovia divaricata 4’-O-β-D-glucosyl-5-O-methylvisamminol RAW264.7 Anti-inflammatory

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https://www.yiyaodaobao.com.cn/EN/10.20047/j.issn1673-7210.2023.02.03 OR https://www.yiyaodaobao.com.cn/EN/Y2023/V20/I2/16

[1] 张晓音，吴旻，李雨萌，等.脂多糖的效应及其机理研究进展[J].动物医学进展，2015，36（12）：133-136.
[2] Chen T，Chen C，Huang Y，et al. Rouhmei.Ethanolic extract of Puhuang（Pollen Typhae） modulates lipopolysaccharide-induced inflammatory response through inducible nitric oxide synthase/cyclooxygenase-2 signaling in RAW 264.7 macrophages [J]. J Tradit Chin Med，2021，41（6）：836-844.
[3] Tebakari M，Daigo Y，Ishikawa H，et al. Anti-inflammatory effect of the water-soluble portion of porcine placental extract in lipopolysaccharide-stimulated RAW264.7 murine mac- rophage cells [J]. Biol Pharm Bull，2018，41（8）：1251-1256.
[4] 周子琪，刘益波，郭珊珊，等.藏紫草醇提物的抗炎作用及其机制[J].中成药，2021，43（11）：3002-3007.
[5] 黄杰，章莹，严婧，等.铁皮石斛多糖对脂多糖诱导的巨噬细胞免疫调节作用[J].中国药学杂志，2017，52（7）：548- 552.
[6] 唐蕾，吴坚，于顾然，等.益气养阴逐瘀方对LPS诱导的RAW264.7细胞炎症模型体外抗炎活性的影响[J].中国实验方剂学杂志，2019，25（1）：141-148.
[7] Zhu L，Gu PQ，Shen H. Protective effects of berberine hydrochloride on DSS-induced ulcerative colitis in rats [J]. Int J Immunopharmacol，2019，68：242-251.
[8] 李依林，薛雪，王海丽，等.不同生长方式防风的HPLC特征图谱和体外抗炎活性比较研究[J].中国现代中药，2021，23（7）：1195-1203.
[9] 刘淇，高铭，吕红君，等.防风乙醇提取物对LPS所致小鼠炎症模型的抗炎作用及机制研究[J].中国中药杂志，2021，46（18）：4800-4807.
[10] 孟达，邓欣，饶高雄，等.余甘子总酚对RAW264.7巨噬细胞源泡沫细胞胆固醇代谢的影响研究[J].中药药理与临床，2022，38（1）：77-81.
[11] 王芳，辛策，宋梦，等.化合物4H对脂多糖诱导RAW264.7细胞炎症的抑制作用及机制研究[J].中国药师，2020， 23（4）：620-623.
[12] 刘静，刘瑛，李宾，等.槐定碱抑制脂多糖诱导的RAW264.7细胞炎症因子分泌及机制[J].细胞与分子免疫学杂志，2015，31（5）：585-589，595.
[13] Wang JZ，Zhang P，He HB，et al. Eburicoic acid from Laetiporus sulphureus（Bull.：Fr.）Murrill attenuates inflammatory responses through inhibiting LPS-induced activation of PI3K/Akt/mTOR/NF-κB pathways in RAW264.7 cells [J]. N-S Arch Pharmacology，2017，390（8）：845-856.
[14] Wu XX，Gao HW，Hou Y，et al. Dihydronortanshinone，a natural product，alleviates LPS-induced inflammatory response through NF-κB，mitochondrial ROS，and MAPK pathways [J]. Toxicol Appl Pharmacol，2018，355：1-8.
[15] 李思媛，崔玉顺，李新星，等.黄精皂苷对脂多糖诱导RAW264.7细胞炎症模型的抗炎作用及其机制[J].中成药，2021，43（10）：2659-2665.
[16] 刘亭，张煜彬，陆定艳，等.紫金龙氯仿-甲醇组分对脂多糖诱导RAW264.7细胞炎症的抑制作用[J].中成药，2018，40（10）：2117-2121.
[17] 韩鸾玮，牛雪妮，吕重宁，等.尚玺胶囊的抗炎作用及其对血清IL-1β、TNF-α和炎症组织中PGE2的影响[J].人参研究，2021，33（6）：35-38.
[18] 王丽君，邓同兴，张景亮，等.白藜芦醇抗炎镇痛作用的COX-2/PGE2信号通路机制研究[J].中药药理与临床，2017，33（4）：35-38.
[19] 曾安琪，华桦，陈朝荣，等.金银花、山银花抗炎药理作用研究[J].中国中药杂志，2020，45（16）：3938-3944.
[20] 丛友权，韩晶晶，方芸，等.雪荔方总黄酮对LPS致RAW264.7细胞炎症的保护作用[J].中成药，2017，39（5）：890-895.
[21] 王青，苏聪平，张惠敏，等.从炎性反应角度探讨清热解毒药的作用机制[J].中国中药杂志，2018，43（18）：3787- 3794.
[22] 贾璐，杭薇，徐幸杰，等.五味子乙素通过抑制TLR4/ NF-κB信号通路减轻PD小鼠炎症反应及DA能神经元凋亡[J].中国免疫学杂志，2022，38（4）：427-431.
[23] 李天萍，黄霞飞，杨蕉，等.呼吸道合胞病毒感染性肺炎患儿的TNF-α和IL-1β水平及TLR4信号通路作用初步探究[J].中华医院感染学杂志，2020，30（6）：919-922.
[24] 钟艳梅，陈坚平，陈淑玲，等.知母皂苷通过调节NF-κB- iNOS-NO信号通路抑制LPS诱导的RAW264.7细胞功能[J].中国药理学通报，2019，35（2）：198-202.
[25] Jiang SX，Yin HN，Li R，et al. The activation effects of fucoidan from sea cucumber Stichopus chloronotus on RAW264.7 cells via TLR2/4-NF-κB pathway and its structure-activity relationship [J]. Carbohydr Polym，2021，270：118353.