基于自噬探讨黄芪多糖对成骨细胞分化的影响

doi:10.20047/j.issn1673-7210.2023.01.04

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Abstract Objective To investigate the effect of milkvetch root polysaccharides on osteoblast differentiation and its related mechanism. Methods Osteoblasts were extracted from the skull of six SPF SD rats (half male and half female) aged one week and weight 15-30 g, and the cells were divided into control group and milkvetch root polysaccharides low, medium, and high dose groups, which were given 0, 0.006 1, 0.012 2, and 0.022 4 g/L milkvetch root polysaccharides for intervention culture, respectively. After 48 h, alkaline phosphatase and alizarin red staining were performed. The cells were transfected with autophagy fluorescent labeled virus, and the transfected cells were divided into control transfection group and milkvetch root polysaccharides low, medium, and high dose transfection groups. The corresponding concentration of milkvetch root polysaccharides was given for intervention culture, and the fluorescence intensity was observed under fluorescence microscope 24 h later. Then the autophagy inhibitor 3-methyladenine (3-MA) (10 mmol/L) and milkvetch root polysaccharides were used to intervene osteoblasts, which were divided into 3-MA group, 3-MA+milkvetch root polysaccharides low dose group, 3-MA+milkvetch root polysaccharides medium dose group, and 3-MA+milkvetch root polysaccharides high dose group. The effect of milkvetch root polysaccharides on the expression of osteogenic differentiation marker genes before and after inhibition autophagy was detected by PCR. Results The proportion of alizarin red staining mineralized area and alkaline phosphatase activity in milkvetch root polysaccharides low, medium, and high dose groups were higher than those in control group, and milkvetch root polysaccharides medium and high dose groups were higher than those in milkvetch root polysaccharides low dose group, and milkvetch root polysaccharides high dose group was higher than that in milkvetch root polysaccharides medium dose group (P＜0.05). The fluorescence intensity of milkvetch root polysaccharides low, medium, and high dose transfection groups were higher than those of control transfection group, and the milkvetch root polysaccharides medium and high dose transfection group were higher than those of milkvetch root polysaccharides low dose transfection group, and the milkvetch root polysaccharides high dose transfection group was higher than that of milkvetch root polysaccharides medium dose transfection group (P＜0.05). The gene expressions of Runt-related transcription factor 2 (RUNX2), bone sialoprotein (BSP), osteocalcin (OCN), and type Ⅰ collagen A1 (COL1A1) in milkvetch root polysaccharides low, medium, and high dose groups were higher than those in control group, and milkvetch root polysaccharides medium and high dose groups were higher than milkvetch root polysaccharides low dose group, milkvetch root polysaccharides high dose group was higher than milkvetch root polysaccharides medium dose group (P＜0.05). There were no significant differences in the expression of RUNX2, BSP, OCN, and COL1A1 in control group, 3-MA group, 3-MA+ milkvetch root polysaccharides low dose group, 3-MA+ milkvetch root polysaccharides medium dose group, and 3-MA+ milkvetch root polysaccharides high dose group (P＜0.05). Conclusion Milkvetch root polysaccharides can promote the differentiation of osteoblasts by increasing the level of autophagy.

Key words： Milkvetch root polysaccharides Osteoblasts Differentiation Autophagy

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	WANG Xiaolei1 WU Ye2 LIU Wenya1 HOU Yanhui1

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WANG Xiaolei1 WU Ye2 LIU Wenya1 HOU Yanhui1. Discussion of effect of milkvetch root polysaccharides on osteoblast differentiation based on autophagy[J]. 中国医药导报, 2023, 20(1): 19-23.

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[1] M?覿kitie O，Zillikens MC. Early-Onset Osteoporosis [J]. Calcif Tissue Int，2022，110（5）：546-561.
[2] Fischer V，Haffner-Luntzer M. Interaction between bone and immune cells：Implications for postmenopausal osteoporosis [J]. Semin Cell Dev Biol，2022，123：14-21.
[3] 唐志宏，段浩，钟宗雨，等.间充质干细胞移植治疗骨质疏松症的机制[J].中国组织工程研究，2022，26（19）：3090- 3094.
[4] Trojani MC，Santucci-Darmanin S，Breuil V，et al. Autophagy and bone diseases [J]. Joint Bone Spine，2022，89（3）：105301.
[5] Behera J，Ison J，Tyagi A，et al. Mechanisms of autophagy and mitophagy in skeletal development，diseases and therapeutics [J]. Life Sci，2022，301：120595.
[6] Fu J，Wang Z，Huang L，et al. Review of the botanical characteristics，phytochemistry，and pharmacology of Astragalus membranaceus（Huangqi）[J]. Phytother Res，2014，28（9）：1275-1283.
[7] Guo MF，Dai YJ，Gao JR，et al. Uncovering the Mechanism of Astragalus membranaceus in the Treatment of Diabetic Nephropathy Based on Network Pharmacology [J]. J Diabetes Res，2020，2020：5947304.
[8] 朱立国，于杰，王尚全.骨康胶囊治疗骨质疏松症临床应用专家共识[J].中华中医药学刊，2022，40（1）：252-258.
[9] 姚顺晗，廖亮，覃家港，等.罗汉果皂苷Ⅴ刺激成骨细胞增殖与分化[J].中国组织工程研究，2019，23（29）：4701- 4706.
[10] Oryan A，Sahvieh S. Effects of bisphosphonates on osteoporosis：Focus on zoledronate [J]. Life Sci，2021，264：118681.
[11] Kendler DL，Cosman F，Stad RK，et al. Denosumab in the Treatment of Osteoporosis：10 Years Later：A Narrative Review [J]. Adv Ther，2022，39（1）：58-74.
[12] Everts-Graber J，Lehmann D，Burkard JP，et al. Risk of Osteonecrosis of the Jaw Under Denosumab Compared to Bisphosphonates in Patients With Osteoporosis [J]. J Bone Miner Res，2022，37（2）：340-348.
[13] Zhao J，Zeng L，Wu M，et al. Efficacy of Chinese patent med- icine for primary osteoporosis：A network meta-analysis [J]. Complement Ther Clin Pract，2021，44：101419.
[14] 张洁帆，许坤，陈元川，等.芪骨胶囊治疗绝经后骨质疏松症的分子机制[J].中国骨质疏松杂志，2021，27（8）：1093-1100.
[15] 李晓明，赵云超，郭东辉，等.坚骨胶囊对胫骨高位截骨术后膝关节功能的近期疗效观察[J].中国中医骨伤科杂志，2019，27（12）：22-25.
[16] Shen G，Shang Q，Zhang Z，et al. Zuo-Gui-Wan Aqueous Extract Ameliorates Glucocorticoid-Induced Spinal Osteoporosis of Rats by Regulating let-7f and Autophagy [J]. Front Endocrinol（Lausanne），2022，13：878963.
[17] Kocak M，Ezazi Erdi S，Jorba G，et al. Targeting autophagy in disease：established and new strategies [J]. Autophagy，2022，18（3）：473-495.
[18] Yin X，Zhou C，Li J，et al. Autophagy in bone homeostasis and the onset of osteoporosis [J]. Bone Res，2019，7：28.
[19] Guo X，Liang M. Metformin alleviates dexamethasone-induced apoptosis by regulating autophagy via AMPK/ mTOR/p70S6K in osteoblasts [J]. Exp Cell Res，2022，415（1）：113120.
[20] Hou C，Wang X，Jiang W，et al. Peptide 11R-VIVIT promotes fracture healing in osteoporotic rats [J]. Int J Mol Med，202，48（2）：162.
[21] Chen T，Gao F，Luo D，et al. Cistanoside A promotes osteogenesis of primary osteoblasts by alleviating apoptosis and activating autophagy through involvement of the Wnt/ β-catenin signal pathway [J]. Ann Transl Med，2022，10（2）：64.
[22] Wei X，Xu A，Shen H，et al. Qianggu capsule for the treatment of primary osteoporosis：evidence from a Chinese patent medicine [J]. BMC Complement Altern Med，2017， 17（1）：108.
[23] Shan H，Zheng X，Li M. The effects of Astragalus Membr- anaceus Active Extracts on Autophagy-related Diseases [J]. Int J Mol Sci，2019，20（8）：1904.
[24] Zhu Y，Su Y，Zhang J，et al. Astragaloside Ⅳ alleviates liver injury in type 2 diabetes due to promotion of AMPK/ mTOR-mediated autophagy [J]. Mol Med Rep，2021，23（6）：437.
[25] Ying Y，Sun CB，Zhang SQ，et al. Induction of autophagy via the TLR4/NF-κB signaling pathway by astragaloside Ⅳ contributes to the amelioration of inflammation in RAW264.7 cells [J]. Biomed Pharmacother，2021，137：111271.