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| Role of FAM83H and its natural antisense transcript FAM83H-AS1 in the occurrence and development of lung adenocarcinoma and ovarian cancer |
| LIU Mengzhen1 SUN Rongrong2 LIU Yanhua2 ZHANG Youwei2 |
1.Clinical College, Xuzhou Medical University, Jiangsu Province, Xuzhou 221009, China;
2.Department of Medical Oncology, Xuzhou Central Hospital, Jiangsu Province, Xuzhou 221009, China |
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Abstract Objective To investigate the biological roles of FAM83H and its natural antisense transcript FAM83H-AS1 in the occurrence and development of tumor, and the regulatory relationship between them. Methods The expression and prognosis of FAM83H and FAM83H-AS1 in various tumor tissues were analyzed by GEPIA online tool. Logarithmic growth stage A549 and SKOV3 cell lines were selected for transfection and divided into blank control group (control group), no-load control group (siNC group) and experimental group (siFAM83H group, SIFAM83H-AS1 group). The cell proliferation, apoptosis, and migration after FAM83H and Fam83H-AS1 knockdown were compared in each group. Results The expressions of FAM83H and FAM83H-AS1 mRNA were up-regulated in various tumor tissues, including lung adenocarcinoma and ovarian serous cystadenocarcinoma. In lung adenocarcinoma, the survival curves of FAM83H mRNA high expression group and FAM83H low expression group were compared, and the difference was statistically significant (P＜0.05). In A549 and SKOV3 cells, the expressions of FAM83H mRNA and protein in siFAM83H group were lower than those in siNC group (P＜0.05); the expression of FAM83H-AS1 mRNA in siFAM83H-AS1 group was lower than that in siNC group (P＜0.05); cell proliferation in siFAM83H group was lower than that in siNC group after transfection for 24, 48 and 72 h (P＜0.05); the cell proliferation ability of siFAM83H-AS1 group was lower than that of siNC group after transfection for 24, 48 and 72 h (P＜0.05); the apoptosis number of siFAM83H group and siFAM83H-AS1 group was higher than that of siNC group (P＜0.05); the number of cell migration in siFAM83H group and siFAM83H-AS1 group was lower than that in siNC group (P＜0.05). After FAM83H knockdown, there was no significant difference in sIFAM83H-AS1 mRNA between siFAM83H group and siNC group (P＞0.05); after FAM83H-AS1 knockdown, there was no statistical significance in FAM83H mRNA and protein between siFAM83H-AS1 group and siNC group (P＞0.05). Conclusion FAM83H and FAM833H-AS1 play the role of oncogenes in the occurrence and development of lung adenocarcinoma and ovarian cancer, but no mutual regulatory relationship has been found between them, and the specific mechanism remains to be further studied.
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