Effect of local injection of hepatocyte growth factor transfected bone marrow mesenchymal stem cells on tendon-bone healing after anterior cruciate ligament reconstruction in rabbits
YANG Deyong1 Kaishaer Baihetiyaer1 JIANG Junyao2 SHU Li1▲
1.The First Department of Joint Surgery, the Sixth Affiliated Hospital of Xinjiang Medical University, Xinjiang Uygur Autonomous Region, Urumqi 830002, China;
2.Basic Medical College, Henan Science and Technology University, Henan Province, Luoyang 471003, China
Abstract Objective To explore the effect of hepatocyte growth factor (HGF) transfected bone marrow mesenchymal stem cell (MSC) on tendon-bone healing after anterior cruciate ligament (ACL) reconstruction in rabbits. Methods Forty-five male New Zealand rabbits (3.0-3.5 kg) aged three to five months were divided into groups A, B, and C by random number table method, with 15 rabbits in each group. All three groups underwent double hindlimb ACL reconstruction, group A did not inject any substance after ACL operation; in group B, MSCs fibrin glue was injected into the tibial canal after operation; in group C, HGF gene modified MSCs fibrin glue was injected into the tibial canal after operation. Six weeks after operation, HE staining was used to observe the tendon-bone healing; the maximum tensile load and the maximum stretching distance of the graft tendon exiting from the tibial canal were tested; the expression of fibroblast growth factor 2 (FGF2) mRNA and SMAD4 mRNA in bone canal and HGF mRNA in joint fluid were detected by RT-PCR. Results Six weeks after operation, the tendon-bone interface of group A was loosely connected and collagen fibers were disordered; in group B, the tendon-bone interface was closely connected and the gap was small, but the arrangement of collagen fibers was not orderly; in group C, the tendon-bone interface was closely connected and the collagen fibers were neatly arranged. The maximum tensile load of group B was higher than that of group A, and that of group C was higher than that of group B (P＜0.05); there was no significant difference in the maximum stretching distance between groups A, B and groups B, C (P＞0.05). The FGF2 mRNA and SMAD4 mRNA in bone canal in group B were higher than those in group A, and those in group C were higher than those in group B (P＜0.05); HGF mRNA of joint fluid in group C was higher than that in group B (P＜0.05). Conclusion Local injection of HGF transfected bone marrow MSCs can enhance tendon-bone healing after ACL reconstruction in rabbits.
YANG Deyong1 Kaishaer Baihetiyaer1 JIANG Junyao2 SHU Li1▲. Effect of local injection of hepatocyte growth factor transfected bone marrow mesenchymal stem cells on tendon-bone healing after anterior cruciate ligament reconstruction in rabbits[J]. 中国医药导报, 2022, 19(35): 28-31.
