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| Effect of Qianlie Xiaozheng Decoction on epithelial-mesenchymal transformation of PC-3 cells in prostate cancer |
| ZHANG Qi YU Huilan LIU Zhaowen QI Leilei GONG Yingjie SONG Shuqi |
| Department of Urinary Surgery, Guang’anmen Hospital, China Academy of Chinese Medical Sciences, Beijing 100053, China |
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Abstract Objective To investigate the effects of Qianlie Xiaozheng Decoction on invasion, migration, and epithelial-mesenchymal transformation of PC-3 cells in prostate cancer. Methods PC-3 cells of prostate cancer were selected as the research object, and the blank group, positive group (PC-SPESⅡ62.3 μl/100 ml), and Qianlie Xiaozheng Decoction low (62.5 μl/100 ml), medium (125.0 μl/100 ml), and high (250.0 μl/100 ml) dose groups were set. The invasion and migration of five groups of cells were observed by invasion and scratch test. Western blot and PCR were used to determine the expression of epithelial-mesenchymal transformation related proteins and genes. Results The invasion cell count and migration rate of Qianlie Xiaozheng Decoction medium and high dose groups were lower than those of the blank group and Qianlie Xiaozheng Decoction low dose group, and the invasion cell count of Qianlie Xiaozheng Decoction high dose group was lower than that of Qianlie Xiaozheng Decoction medium dose group, the differences were statistically significant (P＜0.05 or P＜0.01). The expressions of N-cadherin protein in Qianlie Xiaozheng Decoction low, medium, and high dose groups were lower than those in the blank group, the expressions of SLUG protein in Qianlie Xiaozheng Decoction low and high dose groups were lower than those in the blank group, the expression of SLUG protein in Qianlie Xiaozheng Decoction high dose group was lower than that in the Qianlie Xiaozheng Decoction medium dose group, the expression of E-cadherin protein in Qianlie Xiaozheng Decoction high dose group was higher than those in the blank group, and Qianlie Xiaozheng Decoction low and medium dose groups, the differences were highly statistically significant (P＜0.01). The expressions of SLUG, TWIST, and N-cadherin genes in Qianlie Xiaozheng Decoction low and medium dose groups were lower than those in the blank group, the expression of E-cadherin gene in Qianlie Xiaozheng Decoction high dose group was higher than that in the blank group and Qianlie Xiaozheng Decoction low and medium dose groups, the differences were statistically significant (P＜0.05 or P＜0.01). Conclusion Qianlie Xiaozheng Decoction may inhibit the invasion and migration of tumor cells by regulating the epithelial-mesenchymal transformation of PC-3 cells and delay the progression of metastatic prostate cancer.
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