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| Study on the skin penetration process and mechanism of acupoint application of evodia rutaecarpa mixed with vinegar in the treatment of para-chlorophenylalanine insomnia rats |
| ZHAO Qi1 LUO Lu2 QIAO Yagang3 ZHANG Junwu4 |
1.College of Medicine and Chemical Engineering, Xianyang Vocational Technical College, Shaanxi Province, Xianyang 712000, China;
2.Ward Four of Cancer Hospital, Affiliated Hospital of Shaanxi University of Chinese Medicine, Shaanxi Province, Xianyang 712000, China;
3.Department of General Practice, the Second Affiliated Hospital of Shaanxi University of Chinese Medicine, Shaanxi Province, Xianyang 712000, China; 4.College of Pharmacy, Shaanxi University of Chinese Medicine, Shaanxi Province, Xianyang 712046, China |
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Abstract Objective To explore skin penetration process and mechanism of acupoint application of evodia rutaecarpa mixed with vinegar in the treatment of para-chlorophenylalanine (PACA) insomnia rats. Methods One hundred and sixty-two SPF grade 7-week-old healthy SD male rats were divided into normal group, model group, positive control group (Diazepam 1 mg/kg, 10 ml/kg), small, medium, and large dose groups (doses of 2, 4, 8 g/kg; each group was divided into yǒngquán point group and shénquē point group). There were 10 rats in normal group, model group, and positive control group (Diazepam), and 22 rats in other groups. Ten rats were used to observe the content of the neurotransmitter γ-aminobutyric acid (GABA) in brain tissue and the expression of GABAA1 mRNA, and the other 12 rats were used to observe the penetration process of the acupoint application of evodia rutaecarpa mixed with vinegar in body skin. Except normal group, the other groups were modeled by PCPA method. The rats in the administration group were affixed and administered for 4 h a day, and after 7 d of continuous administration, brain tissue samples were obtained, and the content of the neurotransmitter GABA was detected by enzyme linked immunosorbent assay, and the expression level of GABAA1 mRNA was detected by real-time PCR. After 7 days to 1 h (T1), 4 h after drug (T2), after 8 h (T3), and 8 h after application and 1 h after removal of the drug (T4), the skin of yǒngquán point and shénquē point of rats was frozen and sliced, the penetration process of acupoint application of evodia rutaecarpa mixed with vinegar was observed by confocal laser scanning micrography. Results Compared with model group, GABA content in hypothalamus, brainstem, and hippocampus of rats in large, medium and small dose groups of acupoint application of evodia rutaecarpa mixed with vinegar were increased, and the differences were statistically significant (P＜0.05); GABAA1 mRNA expression of hypothalamus, brainstem, and hippocampus of rats in middle and high dose groups of acupoint application of evodia rutaecarpa mixed with vinegar were increased, and the differences were statistically significant (P＜0.05). The average fluorescence intensity of two acupoint groups with large, medium, and small dose of acupoint application of evodia rutaecarpa mixed with vinegar was compared at different time points, and the differences were statistically significant (P＜0.05); there were statistically significant differences in the average fluorescence intensity on T1-T4 in large, medium, and small dose groups of two acupoints of acupoint application of evodia rutaecarpa mixed with vinegar (P＜0.05). Conclusion Acupoint application of evodia rutaecarpa mixed with vinegar can improve insomnia quality, and the mechanism may be related to the up-regulation of GABA and GABAA1 mRNA expression in brain tissue. It showed good percutaneous permeability in acupoint application of evodia rutaecarpa mixed with vinegar.
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