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| Activation and exhaustion of CD8+ memory T cells in human immunodeficiency virus infected patients after anti-retroviral therapy |
| QIU Wenhao1 XIONG Runsong2 LIU Xian3 WU Yuerong4 XIAO Jian3 |
1.School of Basic Medicine, Guangxi University of Chinese Medicine, Guangxi Zhuang Autonomous Region, Nanning 530022, China;
2.School of Public Health and Management, Guangxi University of Chinese Medicine, Guangxi Zhuang Autonomous Region, Nanning 530022, China; 3.Science and Technology Division, Guangxi University of Chinese Medicine, Guangxi Zhuang Autonomous Region, Nanning 530022, China;
4.Scientific Experiment Center, Guangxi University of Chinese Medicine, Guangxi Zhuang Autonomous Region, Nanning 530022, China |
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Abstract
[Abstract] Objective To investigate the level of activation and exhaustion of CD8+ memory T cells (Tm) in human immunodeficiency virus (HIV) infected patients after anti-retroviral therapy (ART). Methods From January to February 2022, 62 HIV-infected patients (divided into incomplete immune reestablishment group [22 cases] and complete immune reestablishment group [40 ceaes] after ART treatment and 12 healthy subjects were selected in Guigang Center for Disease Control and Prevention, Guangxi. The activation and depletion levels of CD8+Tm cells in HIV-infected patients were detected. Results The expression levels of CD38, HLA-DR, and 2B4 in CD8+Tm cells in the incomplete immune reestablishment group and complete immune reestablishment group were higher than those in the healthy group (P ＜ 0.05). The expression of 2B4 in CD38+HLA-DR+CD8+Tm cells in immune reestablishment group and complete immune-reestablishment group were significantly higher than that in healthy group (P ＜ 0.05). There was a positive correlation between CD38 and HLA-DR expression in CD8+Tm cells of HIV-infected patients (r = 0.634 6, P ＜ 0.05). Conclusion Although the HIV infected patients received the treatment of CD8+Tm cells after art, the activation of CD8+Tm cells still increased, which led to the overexpression of immune exhaustion molecules and the suppression of immune function.
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[1] Hu X，Liang B，Zhou C，et al. HIV-1 late presentation and advanced HIV-1 disease among patients with newly diagnosed HIV-1/AIDS in Southwestern China：a large-scale cross-sectional study [J]. AIDS Res Ther，2019，16（1）：6.
[2] Maeda K，Das D，Kobayakawa T，et al. Discovery and Development of Anti HIV-1 Therapeutic Agents： progress Towards Improved HIV-1 Medication [J]. Curr Top Med Chem，2019，19（18）：1621-1649.
[3] Chew GM，Padua AJP，Chow DC，et al. Effects of Brief Adjunctive Metformin Therapy in Virologically Suppressed HIV-Infected Adults on Polyfunctional HIV-Specific CD8 T Cell Responses to PD-L1 Blockade [J]. AIDS Res Hum Retroviruses，2021，37（1）：24-33.
[4] Munusamy Ponnan S，Hayes P，Fernandez N，et al. Evaluation of antiviral T cell responses and TSCM cells in volunteers enrolled in a phase Ⅰ HIV-1 subtype C prophylactic vaccine trial in India [J]. PLoS One，2020，15（2）：e0229461.
[5] McLane LM，Abdel-Hakeem MS，Wherry EJ. CD8+T cell exhaustion during chronic viral infection and cancer [J]. Annu Rev Immunol，2019，37：457-495.
[6] 中华医学会感染病学分会艾滋病丙型肝炎学组.中国疾病预防控制中心中国艾滋病诊疗指南（2021年版）[J].中国艾滋病性病，2021，27（11）：1182-1201.
[7] 中华中医药学会防治艾滋病分会.艾滋病免疫功能重建不良中西医协同治疗专家共识[J].中医学报，2020，35（2）：281-284.
[8] 陶庄，董继鹏，郭会军，等.青蒿琥酯片对HIV/AIDS患者ART后免疫功能重建不全影响的研究[J].中国艾滋病性病，2021，27（9）：921-925.
[9] 王显凤，王桐桐，张之尧，等.八色组合与二代流式细胞术检测多发性骨髓瘤微小残留病的比较[J].中华血液学杂志，2019，40（6）：512-517.
[10] 杨韵秋，陶鹏飞，谢荣慧，等桂琳.高效抗逆转录病毒疗法治疗艾滋病的效果及对免疫功能的影响[J].中国临床研究，2020，33（4）：505-507，511.
[11] 周明菊.慢性HIV-1感染中CD8记忆前体细胞的特点及与免疫记忆T细胞耗竭机制的研究[D]. 蚌埠：蚌埠医学院，2020.
[12] 陈韵聪，张亚丽，程伟彬，等.未治疗男男性行为者中HIV感染者的病程进展相关因素研究[J].中国艾滋病性病，2020，26（8）：841-843，848.
[13] 辛学娟.长期抗病毒治疗的HIV/AIDS患者HIV-1 DNA与免疫恢复的关系[D].大理：大理大学，2021.
[14] Pincikova T，Paquin-Proulx D，Sandberg JK，et al. Vitamin D treatment modulates immune activation in cystic fibrosis [J]. Clin Exp Immunol，2017，189（3）：359-371.
[15] Files JK，Boppana S，Perez MD，et al. Sustained cellular immune dysregulation in individuals recovering from SARS- CoV-2 infection [J]. J Clin Invest，2021，131（1）：e140491.
[16] Migueles SA，Chairez C，Lin S，et al. Adoptive lymphocyte transfer to an HIV-infected progressor from an elite controller [J]. JCI Insight，2019，4（18）：e130664.
[17] Eckard AR，O’Riordan MA，Rosebush JC，et al. Vitamin D supplementation decreases immune activation and exhaustion in HIV-1-infected youth [J]. Antivir Ther，2018，23（4）：315-324.
[18] Li JZ，Segal FP，Bosch RJ，et al. Antiretroviral Therapy Reduces T-cell Activation and Immune Exhaustion Markers in Human Immunodeficiency Virus Controllers [J]. Clin Infect Dis，2020，70（8）：1636-1642.
[19] Rodríguez-Alba JC，Abrego-peredo A，Gallardo-Hernández C. HIV-1 Disease progression：Overexpression of the Ectoenzyme CD38 as a Contributory Factor? [J] BioEssays，2018，41：1800128.
[20] Lee E，Bacchetti p，Milush J，et al. Memory CD4+T-Cells Expressing HLA-DR Contribute to HIV-1 persistence During prolonged Antiretroviral Therapy [J]. Front Microbiol，2019，10：2214.
[21] Meditz AL，Haas MK，Folkvord JM，et al. HLA-DR+ CD38+CD4+T Lymphocytes Have Elevated CCR5 Expression and produce the Majority of R5-Tropic HIV-1 RNA In Vivo [J]. J Virol，2011，85（19）：10189-200.
[22] Robertson K，Landay A，Miyahara S，et al. Limited correlation between systemic biomarkers and neurocognitive performance before and during HIV treatment [J]. J Neurovirol [J]. 2020，26（1）：107-113.
[23] N’takpe JB，Gabillard D，Moh R，et al. Association between cellular HIV-1 DNA level and mortality in HIV-1 infected African adults starting ART with high CD4 counts[J]. EBioMedicine. 2020，56：102815.
[24] Romani S，Stafford K，Nelson A，et al. Peripheral PD-1+ T Cells Co-expressing Inhibitory Receptors Predict SVR With Ultra Short Duration DAA Therapy in HCV Infection [J]. Front Immunol，2019，10：1470.
[25] Buggert M，Tauriainen J，Yamamoto T，et al. T-bet and Eomes are differentially linked to the exhausted phenotype of CD8+ T cells in HIV infection[J]. PLoS Pathog，2014，10（7）：e1004251.
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