血必净注射液对体外循环后急性肺损伤大鼠肺泡Ⅱ型上皮细胞凋亡相关微RNA表达的影响

doi:10.20047/j.issn1673-7210.2022.31.05

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Abstract Objective To study the effect of Xuebijing Injection on the expression of apoptosis-related microRNA (miRNA) in alveolar typeⅡepithelial cells of cardiopulmonary bypass -induced acute lung injury rats. Methods The candidate miRNAs related to apoptosis of alveolar type Ⅱ epithelial cells were screened by bioinformatics technology; 30 male SD rats (20 to 22 weeks old, body weight 450 to 500 g) were divided into three groups according to the random number table method. Sham operation group, cardiopulmonary bypass group and Xuebijing group. In the sham operation group, 4 ml/kg of normal saline was injected intraperitoneally two hours before the arterial and venous puncture, and in the cardiopulmonary bypass group two hours before the cardiopulmonary bypass. In Xuebijing group, 4 ml/kg of Xuebijing Injection was injected intraperitoneally two hours before cardiopulmonary bypass, and the rats were sacrificed by bloodletting four hours after cardiopulmonary bypass, and lung tissue samples were taken. Lung wet/dry weight ratio (W/D) and alveolar injury quantitative assessment index (IQA) were used to evaluate the degree of lung injury. The effect of Xuebijing Injection on the expression of candidate miRNAs in alveolar type Ⅱ epithelial cells of cardiopulmonary bypass-induced acute lung injury was observed by RT-PCR. Results Five miRNAs related to apoptosis of alveolar type Ⅱ epithelial cells were screened by bioinformatics analysis rno-miR-17-5p, rno-miR-34a-5p, rno-miR-26a-5p, rno-miR-21a-5p, and rno-miR-375-3p. Compared with Sham operation group, W/D and IQA increased in cardiopulmonary bypass group, and the difference was statistically significant (P ＜ 0.05) Compared with the cardiopulmonary bypass group, the W/D and IQA of the Xuebiying group were significantly decreased, and the differences were statistically significant (P ＜ 0.05). The expression of miRNA-17-5p in alveolar typeⅡepithelial cells of cardiopulmonary bypass group was lower than that of sham operation group,and the difference was statistically significant (P ＜ 0.05), while the expression of miRNA-34a-5p was up-regulated (P ＜ 0.05); Compared with the cardiopulmonary bypass group, the expression of miRNA-17-5p in alveolar typeⅡepithelial cells in the XBJ group was up-regulated, and the difference was statistically significant(P ＞ 0.05), while the expression of miR-34a-5p was down- regulated, but there was no statistical difference between the two groups (P ＞ 0.05). Conclusion Xuebijing Injection may play a therapeutic role in the treatment of cardiopulmonary bypass-induced acute lung injury by up-regulating miRNA-17- 5p, and initially reveal the molecular mechanism of Xuebijing injection in the treatment of CPB-Induced acute lung injury.

Key words： Cardiopulmonary bypass Acute lung injury Xuebijing injection MicroRNA-17-5p Bioinformatics technology

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	XU Zhaojun1 ZHANG Shengkang1 ZHANG Yi1 ZHOU Daiyong1 SONG Lan2

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XU Zhaojun1 ZHANG Shengkang1 ZHANG Yi1 ZHOU Daiyong1 SONG Lan2. Effect of Xuebijing Injection on expression of apoptosis-related microRNAs in alveolar type Ⅱ epithelial cells of cardiopulmonary bypass-induced acute lung injury rats[J]. 中国医药导报, 2022, 19(31): 22-26,43.

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https://www.yiyaodaobao.com.cn/EN/10.20047/j.issn1673-7210.2022.31.05 OR https://www.yiyaodaobao.com.cn/EN/Y2022/V19/I31/22

[1] Lagier D，Fischer F，Fornier W，et al. Effect of open-lung vs conventional perioperative ventilation strategies on postoperative pulmonary complications after on-pump cardiac surgery：the PROVECS randomized clinical trial [J]. Intensive Care Med，2019，45：1401-1412.
[2] Huffmyer JL，Groves DS. Pulmonary complications of cardiopulmonary bypass [J]. Best Pract Res Clin Anaesthesiol，2015，29（2）：163-175.
[3] Teng X，Liao J，Zhao L，et al. Whole transcriptome analysis of the differential RNA profiles and associated competing endogenous RNA networks in LPS-induced acute lung injury （ALI） [J]. PLoS One，2021，16（5）：e0251359.
[4] Zhang SQ，Hong YN，Liu HF，et al. miR-584 and miR-146 are candidate biomarkers for acute respiratory distress syndrome [J]. Exp Ther Med，2021 ，21（5）：445.
[5] Guo J，Zhu J，Wang Q，et al. Comparative Efficacy of Seven Kinds of Chinese Medicine Injections in Acute Lung Injury and Acute Respiratory Distress Syndrome：A Network Meta- analysis of Randomized Controlled Trials [J]. Front Pharmacol，2021，12：627751.
[6] 罗鹏，周振兴.血必净对大鼠急性肺损伤的保护作用[J].中国病理生理学杂志，2017，33（2）：132-135.
[7] Gao W，Li N，Cui XG. Efficacy of Xuebijing Injection on Cardiopulmonary Bypass-Associated Pulmonary Injury： A Prospective，Single-center，Randomized，Double Blinded Trial [J]. Chin J Integr Med，2018，24（11）：815- 821.
[8] 徐朝军，宋岚，刘丹薇，等.血必净注射液对Stanford B型主动脉夹层患者腔内复术后炎症细胞因子的影响[J].中国中西医结合急救杂志，2017，24（4）：389-392.
[9] 徐朝军，宋岚，刘丹薇，等.血必净注射液对体外循环心脏直视手术肺保护的临床研究[J].中国急救医学，2017， 37（4）：322-325.
[10] Tang N，Chang J，Zeng Y，et al. Tanshinone ⅡA protects hypoxia-induced injury by preventing microRNA-28 up- regulation in PC-12 cells [J]. Eur J Pharmacol，2019，854：265-271.
[11] 陈思圆，魏劲松，林翰，等.姜黄素通过微小RNA- 122-5p/成纤维细胞生长因子18通路促进骨髓间充质干细胞骨向分化的研究[J].中华麻醉学杂志，2019，39（12）：1522-1525.
[12] Chambers E，Rounds S，Lu Q. Pulmonary Endothelial Cell Apoptosis in Emphysema and Acute Lung Injury [J]. Adv Anat Embryol Cell Biol，2018，228：63-86.
[13] Chu R，Wang J，Bi Y，et al. The kinetics of autophagy in the lung following acute spinal cord injury in rats [J]. Spine J，2018，18（5）：845-856.
[14] Zhang QF，Jia L，Liao XL，et al. The preventive effect of Chinese herbal preparation Xuebijing against hyperactive inflammation after hepato-pancreato-biliary surgery [J]. Transl Med，2019，7（18）：481-491.
[15] Wen TM，Long Q，Quan Z，et al. Xuebijing attenuates decompression-induced lung injuries [J]. Diving Hyperb Med，2020，50（4）：343-349.
[16] 孟繁甦，刘勇，罗泽坤，等.血必净注射液对脓毒症大鼠心肌细胞凋亡保护作用的机制研究[J].中国中医急症，2019，28（3）：418-421.
[17] 贺慧博，郝建，杨炜.血必净对兔急性肺损伤的早期保护作用及其机制研究[J].浙江医学2019，41（17）：1821- 1825.
[18] 卢悦，张平平，王东强，等.急性肺损伤中医病因病机的探讨[J].中国中医急症，2020，29（2）：280-282.
[19] 杨英伟.急性肺损伤/急性呼吸窘迫综合征中医病因病机及辨证分型的研究进展[J].辽宁中医杂志，2013，40（9）：1937-1939.
[20] 苏景深，刘恩顺，赵鑫民.急性肺损伤/急性呼吸窘迫综合征中医药治疗研究进展[J].吉林中医药，2019，39（5）：696-699.
[21] Zheng X，Li H，Chen M，et al. smi-miR396b targeted SmGRFs，SmHDT1，and SmMYB37/4 synergistically regulates cell growth and active ingredient accumulation in Salvia miltiorrhizahairy roots [J]. Plant Cell Rep，2020，39（10）：1263-1283.
[22] Zheng J，Zhu S，Xu H，et al. miR-363-3p inhibits rat lung alveolar type Ⅱ cell proliferation by downregulating?STRA6expression and induces cell apoptosis via cellular oxidative stress and G1-phase cell cycle arrest [J]. Transl Pediatr，2021，10（8）：2095-2105.
[23] Yang C，Yuan W，Yang X，et al. Circular RNA circ-ITCH inhibits bladder cancer progression by sponging miR-17/ miR-224 and regulating p21，PTEN expression [J]. Mol Cancer，2018，17（1）：19.
[24] Lu D，Tang L，Zhuang Y，et al. miR-17-3P regulates the proliferation and survival of colon cancer cells by targeting Par4 [J]. Mol Med Rep，2018，17（1）：618-623.
[25] Gunasekaran M，Xu Z，Nayak DK，et al. Donor-Derived Exosomes With Lung Self-Antigens in Human Lung Allograft Rejection [J]. Am J Transplant，2017，17（2）：474- 484.