Effects of Cuscutae Semen-Lycii Fructus on proliferation and apoptosis in Triperygium wilfordii multiglucoside induced spermatocytic dysfunction model of spermatogonium
WANG Jisheng BAO Binghao DENG Sheng FENG Junlong LI Haisong
Department of Andrology, Dongzhimen Hospital, Beijing University of Chinese Medicine, Beijing 100700, China
Abstract Objective To investigate the ameliorative effect and mechanism of couplet medicines traditional Chinese medicine of Cuscutae Semen-Lycii Fructus on proliferation and apoptosis of spermatogenic dysfunction induced by Tripterygium wilfordii multiglucoside. Methods Twenty SPF 6-week-old (200±20) g male SD rats were divided into blank group, Tripterygium wilfordii multiglucoside group, L-carnitine group, and couplet medicines group by the random number table method, with five rats in each group, and were used to prepare drug-containing serum. Spermatogonium were divided into blank group, model group, L-carnitine group, and couplet medicines group. The blank group was treated with 10% blank rat serum, the model group, L-carnitine group, and couplet medicines group were firstly treated with 10% Tripterygium wilfordii multiglucoside intragastric administration of drug-containing serum to prepare spermatogenic dysfunction model, and then L-carnitine group and couplet medicines group were treated with 10% drug-containing serum of L-carnitine and Cuscutae Semen-Lycii Fructus respectively. The changes of cell proliferation, apoptosis, ultrastructure and mitochondrial membrane potential were observed. Results Compared with blank group, the apoptosis rate of model group was significantly increased (P ＜ 0.01). Compared with the model group, the apoptosis rate of the couplet medicines group was significantly decreased (P ＜ 0.01). Compared with blank group, the percentage of cells in G0/G1 and G2/M phase in model group was significantly decreased, and the percentage of cells in S phase was significantly increased (P ＜ 0.05 or P ＜ 0.05). Compared with the model group, the percentage of cells in G0/G1 and G2/M phases was significantly increased, and the percentage of cells in S phase was significantly decreased in the couplet medicines group (P ＜ 0.01). Compared with the blank group, the mitochondrial membrane potential in the model group was significantly increased (P ＜ 0.01). Compared with the model group, the mitochondrial membrane potential in the couplet medicines group was significantly decreased (P ＜ 0.01). In the blank group, the morphology and structure of the organelles were normal, while in the model group, the cell membrane was ruptured in a large area, the density of the cell matrix was decreased in a large area, and vacuolation was observed. The cell membrane structure of L-carnitine group and couplet medicines group was intact, and the mass density of cell matrix was normal. Conclusion Cuscutae Semen-Lycii Fructus can regulate spermatogenic function of spermatogonium, which may be related to the regulation of cell proliferation, apoptosis, cell cycle and mitochondrial membrane potential.
WANG Jisheng BAO Binghao DENG Sheng FENG Junlong LI Haisong. Effects of Cuscutae Semen-Lycii Fructus on proliferation and apoptosis in Triperygium wilfordii multiglucoside induced spermatocytic dysfunction model of spermatogonium[J]. 中国医药导报, 2022, 19(31): 5-9.
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