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| Effects of Cuscutae Semen-Lycii Fructus on proliferation and apoptosis in Triperygium wilfordii multiglucoside induced spermatocytic dysfunction model of spermatogonium |
| WANG Jisheng BAO Binghao DENG Sheng FENG Junlong LI Haisong |
| Department of Andrology, Dongzhimen Hospital, Beijing University of Chinese Medicine, Beijing 100700, China |
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Abstract Objective To investigate the ameliorative effect and mechanism of couplet medicines traditional Chinese medicine of Cuscutae Semen-Lycii Fructus on proliferation and apoptosis of spermatogenic dysfunction induced by Tripterygium wilfordii multiglucoside. Methods Twenty SPF 6-week-old (200±20) g male SD rats were divided into blank group, Tripterygium wilfordii multiglucoside group, L-carnitine group, and couplet medicines group by the random number table method, with five rats in each group, and were used to prepare drug-containing serum. Spermatogonium were divided into blank group, model group, L-carnitine group, and couplet medicines group. The blank group was treated with 10% blank rat serum, the model group, L-carnitine group, and couplet medicines group were firstly treated with 10% Tripterygium wilfordii multiglucoside intragastric administration of drug-containing serum to prepare spermatogenic dysfunction model, and then L-carnitine group and couplet medicines group were treated with 10% drug-containing serum of L-carnitine and Cuscutae Semen-Lycii Fructus respectively. The changes of cell proliferation, apoptosis, ultrastructure and mitochondrial membrane potential were observed. Results Compared with blank group, the apoptosis rate of model group was significantly increased (P ＜ 0.01). Compared with the model group, the apoptosis rate of the couplet medicines group was significantly decreased (P ＜ 0.01). Compared with blank group, the percentage of cells in G0/G1 and G2/M phase in model group was significantly decreased, and the percentage of cells in S phase was significantly increased (P ＜ 0.05 or P ＜ 0.05). Compared with the model group, the percentage of cells in G0/G1 and G2/M phases was significantly increased, and the percentage of cells in S phase was significantly decreased in the couplet medicines group (P ＜ 0.01). Compared with the blank group, the mitochondrial membrane potential in the model group was significantly increased (P ＜ 0.01). Compared with the model group, the mitochondrial membrane potential in the couplet medicines group was significantly decreased (P ＜ 0.01). In the blank group, the morphology and structure of the organelles were normal, while in the model group, the cell membrane was ruptured in a large area, the density of the cell matrix was decreased in a large area, and vacuolation was observed. The cell membrane structure of L-carnitine group and couplet medicines group was intact, and the mass density of cell matrix was normal. Conclusion Cuscutae Semen-Lycii Fructus can regulate spermatogenic function of spermatogonium, which may be related to the regulation of cell proliferation, apoptosis, cell cycle and mitochondrial membrane potential.
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[1] Katz DJ，Teloken P，Shoshany O. Male infertility-The other side of the equation [J]. Aust Fam Physician，2017，46（9）：641-646.
[2] 王继升.补肾生精法治疗少弱精子症的数据挖掘及其调控生精细胞的机制研究[D].北京：北京中医药大学，2021.
[3] 王继升，邓省，代恒恒，等.基于数据挖掘分析李海松教授治疗少弱精子症用药特点与规律[J].世界中西医结合杂志，2021，16（2）：245-248，253.
[4] 翁浩伟，冯家明，陈琦，等.周少虎教授运用益肾活血方治疗畸形精子症经验[J].中国医药导报，2020，17（17）：162-164，168.
[5] 张硕，吴嘉瑞，刘鑫馗.基于数据挖掘中医治疗精液异常用药规律研究[J].世界中医药，2017，12（7）：1688-1692.
[6] 林基伟，陈佳，黄钦展，等.不育方剂的组方规律数据挖掘分析[J].中国实验方剂学杂志，2016，22（4）：218-221.
[7] Wang J，Bao B，Meng F，et al. To study the mechanism of Cuscuta chinensis Lam. And Lycium barbarum L. in the treatment of asthenospermia based on network pharmacology [J]. J Ethnopharmacol，2021，270：113790.
[8] 管斯琪，祝雨田，董雷，等.“菟丝子-枸杞子”药对修复生精功能障碍大鼠血睾屏障的机制研究[J].中国性科学，2020，29（2）：100-107.
[9] Wang J，Bao B，Meng F，et al. In vitro and in vivo investigation of the therapeutic mechanism of Lycium Chinense and Cuscutae Semen on oligoasthenozoospermia [J]. Andrologia，2021，53（5）：e14014.
[10] 张开舒，周芳，安琪，等.麒麟丸对少弱精子症模型雄性大鼠生殖功能的保护作用研究[J].中华男科学杂志，2017， 23（9）：821-827.
[11] 昊霞，王忠震，林兵，等.雷公藤毒性作用机制研究进展[J].中国医院药学杂志，2015，35（16）：1519-1523.
[12] Yan L，Bian L，Pang P，et al. Research progress on protective effect and mechanism of Lycium barbarum polysaccharide on the male reproductive system [J]. TMR Aging，2019，1（2）：25-31.
[13] Zhu W，Zhou Sx，Liu JH，et al. Prebiotic，immuno-stimulating and gut microbiota-modulating effects of Lycium barbarum polysaccharide [J]. Biomed Pharmacother，2020， 121（1）：109591.
[14] Jin ML，Huang QS，Zhao K，et al. Biological activities and potential health benefit effects of polysaccharides isolated from Lycium barbarum L [J]. Int J Biol Macromol，2013，54（3）：16-23.
[15] 姜璘.氯化锰对小鼠精原干细胞的损伤及机制研究[D].遵义：遵义医科大学，2021.
[16] 杜星德. PI3K/AKT/mTOR通路在微囊藻毒素-LR致小鼠精原细胞恶性转化中的作用及机制[D].郑州：郑州大学，2021.
[17] Xu Y，Dong H，Yang W. Regulators in the apoptotic pathway during spermatogenesis：Killers or guards? [J]. Gene，2016， 582（2）：97-111.
[18] 曹宇华，殷舞，吕艳茹.敲低鞘氨醇激酶-1对非小细胞肺癌细胞增殖及线粒体凋亡途径的影响及机制[J].生理学报，2021，73（6）：893-900.
[19] Antico Arciuch VG，Elguero ME，Poderoso JJ，et al. Mitochondrial regulation of cell cycle and proliferation [J]. Antioxid Redox Signal，2012，16（10）：1150-1180.
[20] 陈娟，陈拉斯，陈丽，等.FOXM1通过靶向上调PHB2的转录改善线粒体功能障碍保护脂多糖诱导的肾小管上皮细胞损伤[J].河北医学，2022，28（3）：368-374.
[21] Guthrie HD，Welch GR. Effects of reactive oxygen species on sperm function [J]. Theriogenology，2012，78（8）：1700- 1708.
[22] 仲崇副，韩斌，纪云，等.线粒体能量代谢与弱精子症的关系研究进展[J].世界中医药，2021，16（4）：666-671.
[23] Agnihotri SK，Agrawal AK，Hakim BA，et al. Mitochondrial membrane potential（MMP）regulates sperm motility [J]. In Vitro Cell Dev Biol Anim，2016，52（9）：953-960.
[24] 傅龙龙，宋小芬，张开舒，等.冷冻复苏对人精子线粒体的影响[J].中华男科学杂志，2020，26（11）：984-988.
[25] 宾东华，李玲，王磊，等.基于AMPK/PPARα信号通路研究知柏地黄汤对解脲脲原体感染大鼠生精细胞能量代谢的影响[J].中华男科学杂志，2021，27（5）：437-444.
[26] 王军可.线粒体—内质网结构偶联介导线粒体钙离子超载在DEHP致未成熟睾丸发育损伤中的作用及机制研究[D].重庆：重庆医科大学，2021.
[27] 徐冰冰，张家新，王瑞军，等.哺乳动物精子线粒体研究进展[J].中国畜牧兽医，2019，46（9）：2651-2657.
[28] 颜贝，王娟，周岳，等.枸杞多糖对冻存精子线粒体保护作用的研究[J].时珍国医国药，2020，31（9）：2110-2113. |
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