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| The effect of siRNA interference with Rac1 expression on proliferation and migration of human lens epithelial cells |
| SUN Guoqing TANG Jianming▲ |
| Department of Ophthalmology, Baoshan District Hospital of Integrated Chinese and Western Medicine of Shanghai City, Shanghai 200940, China |
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Abstract Objective To study the effect of Rac1 on the proliferation and migration of human lens epithelial cells SRA01/04, and to explore the role of Rac1 in the development and progression of posterior capsular opacities and capsular contraction syndrome. Methods SRA01/04 cells were divided into mock group and siRac1 group. Rac1-related siRNA was constructed and transiently transfected in siRac1 group cells. Western blot was used to detect the changes of Rac1 protein expression, CCK was used to detect the changes of cell growth cycle, flow cytometry was used to detect the cell cycle and apoptosis, Transwell was used to detect the effect of Rac1 on cell migration ability. Results The absorbance values of the two groups at 72 hours were higher than those at 24 hours (P ＜ 0.05), the absorbance values of siRac1 group at 48, 72 hours were lower than those of mock group (P ＜ 0.05). Compared with mock group, the relative expression of mRNA and protein in siRac1 group decreased significantly, the percentage of G1 phase cells increased significantly, while the percentage of S phase cells decreased significantly, the number of cells passing through the membrane reduced significantly, the differences were statistically significant (P ＜ 0.05). F-actin staining showed that the forming ability of pseudopodium in siRac1 group was significantly weaker than that of mock group. Conclusion Rac1 may affect the occurrence and development of posterior capsular opacities and capsular contraction syndrome through adjusting the proliferation and migration of lens epithelial cells.
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