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| Research of the influence of Doxorubicin loaded melanin particles on drug resistance of anaplastic thyroid cancer cells |
| ZHANG Yuchun1,2 ZHANG Qing1 YANG Yu1 ZHAO Yijing1 WANG Kun1 MA Xianghua2 |
1.Department of Endocrinology, the Affiliated Jiangning Hospital of Nanjing Medical University, Jiangsu Province, Nanjing 210000, China;
2.Department of Endocrinology, the First Affiliated Hospital of Nanjing Medical University, Jiangsu Province, Nanjing 210000, China |
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Abstract Objective To research the influence of Doxorubicin loaded melanin nanoparticles (DOX-MNPs) on drug resistance of anaplastic thyroid cancer (ATC) cells. Methods Dopamine-melanin nanoparticles (MNPs) were synthesized as previously reported and Doxorubicin (DOX) was loaded onto MNPs. The loading efficiency and capacity of MNPs at different mass ratios of DOX to MNPs were determined by UV-Vis spectroscopy and the characterization of nanoparticles was acquired by transmission electron microscopy (TEM) and Brookhaven Zeta PALS analyzer. HTh74 and HTh74R cells were incubated with various concentrations of free DOX and DOX-MNPs (0, 10, 20, 40, 80, 160 mg/L). Cell viability was determined by MTT, and intracellular DOX fluorescent signal was determined by flow cytometry studies. Results The MNPs was successfully synthesized and the loading efficiency of DOX-MNPs decreased with the increasing mass ratio of DOX to MNPs. The loading efficacy peaked at 93.45% when the mass ratio was 0.167:1. At this mass ratio, the obtained DOX-MNPs had similar morphology to MNPs, and zeta potential and size distribution of DOX-MNPs were 13.79 mV and (241.1±4.8) nm. In HTh74R cells, when the concentration of DOX-MNPs was higher than 20 mg/L, the cell viability was significantly lower than that of the equivalent drug concentration (P < 0.05), and the uptake rate of DOX-MNPs was significantly higher than that of free DOX (P < 0.05). The degree of uptake was similar to that of HTh74 cells. Conclusion The cellular uptake capacity and therapeutic effect of the DOX-MNPs are significantly higher than those of free DOX at the same drug concentration in drug-resistant HTh74R cells.
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