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| Effect of Fluoxetine on synaptic plasticity of optic cortical neuron cells in monocular deprived amblyopia rats and its related mechanism |
| LUO Xianling WU Luoling#br# |
Department of Ophthalmology, the First People’s Hospital of Fuyang District, Zhejiang Province, Hangzhou 311400, China
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Abstract Objective To investigate the effect of Fluoxetine on synaptic plasticity of optic cortical neuron cells in monocular deprived amblyopia rats and its related mechanism. Methods Ten of 60 SPF male SD rats were selected as the control group, and the other 50 rats were used to construct the monocular deprived amblyopia rat model. The 50 rats with successful modeling were divided into positive drug group, model group, low, medium, and high dose groups by random number table method, with 10 rats in each group. The low, medium, and high dose groups were intraperitoneally injected with 5, 10, and 20 mg/(kg·d) Fluoxetine; the positive drug group was intraperitoneally injected with 80 mg/(kg·d) Levodopa; and the model group and the control group were intraperitoneally injected with the same amount of normal saline. Each group was treated for 28 days. P100 latency and P100 amplitude in each group were measured; the ultrastructural changes of synapses of neurons in visual cortex in each group were observed; the ERK1/2, p-ERK1/2, CREB, and p-CREB protein in visual cortex in each group were detected. Results The P100 latency, synaptic gap, and synaptic density thickness in the model group were higher than those in the control group, and the P100 amplitude and p-ERK1/2/ERK1/2, and p-CREB/CREB in visual cortex were lower than those in the control group (P ＜ 0.05). The P100 latency, synaptic gap, and synaptic density thickness in the low, medium, and high dose groups were lower than those in the model group, and the P100 amplitude, p-ERK1/2/ERK1/2, and p-CREB/CREB in the visual cortex were higher than those in the model group; the P100 latency, synaptic space, and synaptic density thickness in the middle and high dose groups were lower than those in the low dose group, and the P100 amplitude, p-ERK1/2/ERK1/2, and p-CREB/CREB in the visual cortex were higher than those in the low dose group; the P100 latency, synaptic gap, and synaptic density thickness in the high-dose group were lower than those in the medium dose group, and the P100 amplitude, p-ERK1/2/ERK1/2, and p-CREB/CREB in the visual cortex were higher than those in the medium dose group (P ＜ 0.05). Compared with the model group, the synaptic structure of the visual cortex neurons in the low, medium, and high dose groups was slightly clear, and the myelin sheath lamellar structure was clear. Conclusion Fluoxetine can remodel the synaptic ultrastructure of visual cortex neurons in monocular deprived amblyopic rats, activate the structural plasticity of visual cortex, and improve the visual function of rats, which may be achieved by activating ERK/CREB pathway.
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