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Application of hypervariable octameric oligonucleotide finger-prints genotyping on molecular epidemiologic study of Brucellosis in Shijiazhuang |
ZHANG Zhi1 GAO Huixia1 TIAN Yueyang1 LI Yanfeng2 ZHENG Liheng3 LI Yanan4 |
1.Department of Clinical Laboratory, the Fifth Hospital of Shijiazhuang, Hebei Province, Shijiazhuang 050021, China;
2.Department of Clinical Laboratory, Hebei Province Veterans Hospital, Hebei Province, Baoding 071000, China;
3.Department of Clinical Laboratory, Hebei Chest Hospital, Hebei Province, Shijiazhuang 050000, China;
4.Department of Clinical Diagnosis, Pingan Hospital, Hebei Province, Shijiazhuang 050000, China |
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Abstract Objective To explore the application of hypervariable octameric oligonucleotide finger-prints (HOOF-Prints) genotyping on molecular epidemiologic study of Brucellosis in Shijiazhuang. Methods A total of 145 patients with confirmed Brucellosis admitted to the Fifth Hospital of Shijiazhuang from January 2016 to December 2018 were selected, Brucella strains were isolated and cultured. HOOF-Prints genotyping was used for molecular epidemiologic study. Results The most prevalent species of Brucella in Shijiazhuang were B.melitensis and B.abortus. Among them, B.melitensis biover 3 and B.melitensis biover 2 were the main epidemic strains. Conclusion The main epidemic strains of Brucella in Shijiazhuang are B.melitensis biover 3 and B.melitensis biover 2. HOOF-Prints genotyping is an effective method for the molecular epidemiologic study on Brucella.
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