IGF1基因慢病毒转染骨髓间充质干细胞治疗骨性关节炎的实验研究

doi:10.20047/j.issn1673-7210.2022.28.03

Abstract
Figure/Table
References (18)
Related Citation (15)

Download: PDF (1607 KB) HTML (1 KB)
Export: BibTeX | EndNote (RIS)

Abstract Objective To investigate the effect of IGF1 gene lentivirus transfected bone marrow mesenchyml stem cells (BMSCs) in the treatment of osteoarthritis (OA) based on animal experiments. Methods A total of 40 SPF male Wistar rats (10-12 week old, weighing 280-320 g) were divided into BMSCs+IGF1 group, BMSCs group and model group according to the random number table method, with ten rats in each group. The OA model was established by anterior cruciate ligament resection of knee joint, and the other ten rats were used for BMSCs isolation. The 0.1 ml IGF1 gene lentivirus transfected BMSCs (1×107 cells) were injected into knee joint cavity in rats of BMSCs+IGF1group. The 0.1 ml BMSCs (1×107 cells) were injected into knee joint cavity in rats of BMSCs group. The 0.1 ml normal saline were injected into knee joint cavity in rats of model group. The serum levels of C-terminal peptide of type Ⅰ collagen (CTX-Ⅰ), CTX-Ⅱ, interleukin (IL)-1α, and IL-6 in rats were determined by enzyme-linked immunosorbent assay. The mRNA expression levels of osteocalcin (OC), glycogen synthase kinase-3β (GSK-3β), alkaline phosphatase (ALP), and Runt-associated transcription factor 2 (Runx2) in the knee cartilage tissues of rats were detected by real-time quantitative polymerase chain reaction. The protein expression levels of β-catenin,Wnt1, and GSK-3β in the knee cartilage tissues of rats were detected by Western blot. Results BMSCs surface antigens CD29,CD34,CD45, and CD105 were highly expressed, and the transfection efficiency of lentivirus was (90.36±2.18)%. The knee cartilage of rats was intact without obvious degeneration and the articular surface was flat in BMSCs+IGF1 group. The other two groups had varying degrees of cartilage degeneration and uneven articular surface. Compared with the model group, the serum levels of CTX-Ⅰ, CTX-Ⅱ, IL-1α, and IL-6, the mRNA expression levels of OC, GSK-3β, ALP, and Runx2, the protein expression levels of β-catenin, Wnt1, and GSK-3β were decreased in the BMSCs group, the differences were statistically significant (P ＜ 0.05). Compared with the BMSCs group, the above indexes in the BMSCs+IGF1 group were decreased, the differences were statistically significant (P ＜ 0.05). Conclusion IGF1 gene lentivirus transfected BMSCs can effectively improve the pathological injury of OA rats, and significantly promote the repair of knee joint injury.

Key words： Osteoarthritis Bone marrow mesenchyml stem cells IGF1 gene Lentivirus Rats Animal experiments

	Service

	E-mail this article
	Add to my bookshelf
	Add to citation manager
	E-mail Alert
	RSS
	Articles by authors
	XIANG Wenyuan1 DENG Yingjie2 LIAO Jun2 FANG Rui2

Cite this article:

XIANG Wenyuan1 DENG Yingjie2 LIAO Jun2 FANG Rui2. Experimental study of IGF1 gene lentivirus transfected bone marrow mesenchyml stem cells in the treatment of osteoarthritis[J]. 中国医药导报, 2022, 19(28): 17-22.

URL:

https://www.yiyaodaobao.com.cn/EN/10.20047/j.issn1673-7210.2022.28.03 OR https://www.yiyaodaobao.com.cn/EN/Y2022/V19/I28/17

[1] 杨山辉，陶树青.膝关节骨性关节炎的非手术治疗进展[J].东南大学学报（医学版），2017，36（4）：677-680.
[2] 廖德发.我国骨性关节炎流行病学调查现状[J].微创医学，2017，12（4）：521-524.
[3] Zhang LL，Li SL，Li J，et al. LncRNA ORLNC1 Promotes Bone Marrow Mesenchyml Stem Cell Pyroptosis Induced by Advanced Glycation End Production by Targeting miR-200b-3p/Foxo3 Pathway [J]. Stem cell reviews and reports，2021，17（6）：2262-2275.
[4] 王宪峰，欧昕，邓必勇.不同来源间充质干细胞外泌体治疗骨关节炎疗效的比较[J].中国组织工程研究，2022，26（25）：3980-3985.
[5] 孙庆云，闫振宇.间充质干细胞对骨关节炎修复机制的研究进展及应用[J].中国实验动物学报，2021，29（2）：262-267.
[6] 王菲，蔡晓庆，史亮，等，马凌.IGF-1基因转染对脂肪间充质干细胞活性的影响及机制研究[J].现代生物医学进展，2016，16（12）：2241-2244，2253.
[7] 何强，尹宏，代凤雷，等.早期膝骨性关节炎模型大鼠的建立与验证[J].中国组织工程研究，2019，23（27）：4338-4343.
[8] Kovács B，Vajda E，Nagy EE. Regulatory Effects and Interactions of the Wnt and OPG-RANKL-RANK Signaling at the Bone-Cartilage Interface in Osteoarthritis [J]. Int J Mol Sci，2019，20（18）：4653.
[9] Yusuke W，Atsunori T，Satoshi S，et al. Mesenchymal Stem Cells and Induced Bone Marrow-Derived Macrophages Synergistically Improve Liver Fibrosis in Mice [J]. Stem Cells Transl Med，2019，8（3）：271-284.
[10] 姜明宇，任明永，高莹，等.重组慢病毒介导STAT3过表达质粒转染促进显性脊柱裂胎鼠骨髓间充质干细胞向神经细胞分化[J].河北医学，2021，27（3）：384-389.
[11] 刘红升，赵晓东，苏琴，等.人IGF-I基因慢病毒介导RNA干扰有效靶点的设计及筛选[J].中国急救医学，2012，32（5）：421-426，彩1.
[12] 段志斌，仇志强，彭鲲，等.Runx2慢病毒转染骨髓间充质干细胞修复骨性关节炎的关节损伤[J].中国组织工程研究，2018，22（29）：4607-4613.
[13] 赵伊婷，何云，马洁.CTX-Ⅱ检测对早期KOA患者的诊断价值研究[J].河北医药，2021，43（17）：2580-2584.
[14] 张琼，侯景丽，李立军，等.膝关节镜术后结合活血祛瘀汤内服对膝关节炎患者骨代谢指标及炎性指标的影响[J].世界中医药，2019，14（4）：988-992.
[15] 汪国翔，章晓云.骨关节炎病变过程中炎症细胞因子及相关信号通路的作用机制[J].中国组织工程研究，2021，25（14）：2266-2273.
[16] 钟晓辉，曾蔚林，陈春松，等.独活寄生汤对膝骨关节炎患者的疗效、炎症水平及中医证候积分的影响[J].中国当代医药，2022，29（13）：122-125.
[17] 付兴前，郭礼跃，欧梁，等.基于数据挖掘和网络药理学的肝肾亏虚型膝骨关节炎的用药规律及机制分析[J].世界中医药，2022，17（5）：736-742.
[18] 王迷娜，刘璐，赵洛鹏，等.膝骨关节炎炎性因子及信号通路的研究进展[J].中国骨伤，2020，33（4）：388-392.