基于p38 MARK信号通路探讨益气生肌合剂联合三乙醇胺乳膏对压疮大鼠模型皮损及血清炎症因子的影响

doi:10.20047/j.issn1673-7210.2022.25.05

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Abstract Objective To investigate the effect and mechanism of Yiqi Shengji Mixture combined with Triethanolamine Cream on skin lesions and serum inflammatory factors in a rat model of pressure ulcer. Methods Fifty SPF SD rats (half male and half male, 6-7 weeks old, 180-200 g) were divided into sham operation group, model group, Triethanolamine Cream group, Yiqi Shengji group, and combined medication group by random number table method, with 10 rats in each group. The model group, Triethanolamine Cream group, Yiqi Shengji group, combined medication group were simulated by ischemia-reperfusion method. in the sham operation group, the magnet was only embedded in the same site without pressure. Sham operation group and model group were given normal saline 0.4 ml by gavage; Triethanolamine Cream group was given Triethanolamine Cream for external use; Yiqi Shengji group was given 12.98 g/(kg·d) Yiqi Shengji Mixture by gavage; combined medication group received Triethanolamine Cream for external use and Yiqi Shengji Mixture by gavage, all groups were given for eight days. Wound conditions, serum inflammatory factor levels, and wound P38 MARK signaling pathway related protein expression were recorded in each group. Results The skin lesion area of Triethanolamine Cream group, Yiqi Shengji group, and combined medication group on the 8th day of modeling was lower than that of model group, and the skin lesion area of combined medication group on the 8th day of modeling was lower than that of Triethanolamine Cream group and Yiqi Shengji group(P ＜ 0.05). The serum levels of interleukin (IL)-2, IL-12, and C-reactive protein (CRP) in the model group were higher than those in the sham operation group (P ＜ 0.05). The serum inflammatory factor levels of IL-2, IL-12, and CRP in Triethanolamine Cream group, Yiqi Shengji group and combined medication were lower than those in model group, and the serum inflammatory factor levels of IL-2, IL-12, and CRP in combined medication group were lower than those in Triethanolamine Cream group and Yiqi Shengji group (P ＜ 0.05). The protein expressions of p38mitogen-activatedproteinkinase (p38 MARK), vascular endothelial growth factor (VEGF), and matrix metalloproteinases (MMP-9) in the skin lesions of the sham operation group were lower than those of the model group (P ＜ 0.05). The protein expression levels of p38 MARK, VEGF, and MMP-9 in skin lesions in Triethanolamine Cream group, Yiqi Shengji group and combination drug group were lower than those in model group, and the protein expression levels of p38 MARK, VEGF, and MMP-9 in skin lesions in combination drug group were lower than those in Triethanolamine Cream group and Yiqi Shengji group (P ＜ 0.05). Conclusion The combination of Yiqi Shengji Mixture and Triethanolamine Cream can effectively repair the skin lesions of the pressure ulcer rat model and reduce the level of serum inflammatory factors.

Key words： Pressure ulcer;Yiqi Shengji Mixture p38 MARK signal pathway Inflammatory factor Mechanism study

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	TANG Yuli1 LIU Xin1 LIAO Ruoyi2

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TANG Yuli1 LIU Xin1 LIAO Ruoyi2. Discussion on the effect of Yiqi Shengji Mixture combined with Triethanolamine Cream on skin lesions and serum inflammatory factors in pressure ulcer rat model based on p38 MARK signaling pathway[J]. 中国医药导报, 2022, 19(25): 24-27,37.

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https://www.yiyaodaobao.com.cn/EN/10.20047/j.issn1673-7210.2022.25.05 OR https://www.yiyaodaobao.com.cn/EN/Y2022/V19/I25/24

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