C-末端Src蛋白/黏着斑激酶/上皮钙黏素通路在宫颈癌侵袭转移中的作用及机制研究

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Abstract Objective To study the role and mechanism of C-terminal Src protein (c-Src) / focal adhesion kinase (FAK) / E-cadherin (E-cad) pathway in invasion and metastasis of cervical cancer. Methods Thirty cervical squamous cell carcinoma (CSCC) tissue samples and 30 normal cervical (NC) tissue samples were collected from the First Affiliated Hospital of Hunan University of Medicine from January 2019 to December 2020. Immunohistochemical stain was used to detect the positive rate of E-cad in CSCC and NC tissue samples. The relationship between E-cad and clinicopathological features of CSCC patients was analyzed. Western blot was used to detect the protein expression levels of c-Src, FAK, and E-cad in CSCC and NC tissues. In addition, cervical cancer Caski cell lines were selected and divided into Caski group and Caski+ c-SRC inhibitor group. The Caski group was not treated, and the Caski+c-SRC inhibitor group was added with c-Src inhibitor (2.7 nmol/L). The migration, invasion, and apoptosis of the two groups were observed, and the protein expression levels of c-Src, FAK, and E-cad in the two groups were detected by Western blot. Results The positive rate of E-cad in CSCC tissue samples was lower than that in NC, the difference was statistically significant (P ＜ 0.05). E-cad expression was correlated with International Federation of Gynecology and Obstetrics staging, differentiation degree, and recurrence of CSCC patients (P ＜ 0.05). The expression levels of c-Src and FAK protein in CSCC tissue samples were higher than those in NC tissue samples, while the expression level of E-cad protein was lower than that in NCT, the differences were statistically significant (P ＜ 0.05). The count of invaded and migrated cells and the expression levels of c-Src and FAK protein in Caski+c-Src inhibitor group were lower than those in Caski group, and the apoptosis rate and expression level of E-cad protein were higher than those in Caski group, the differences were statistically significant (P ＜ 0.05). Conclusion C-src may lead to decreased invasion and migration ability of cervical cancer cells by up-regulating FAK and down-regulating E-cad, the mechanism may be related to increased apoptosis.

Key words： Cervical squamous cell carcinoma Invasion Migration C-terminal Src protein Focal adhesion kinase E-cadherin

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	TANG Qunhua MU Xiaoqin HUANG Shilan TANG Zhikang LIAN Xiaoling▲

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TANG Qunhua MU Xiaoqin HUANG Shilan TANG Zhikang LIAN Xiaoling▲. Study of the role and mechanism of C-terminal Src protein / focal adhesion kinase / E-cadherin pathway in invasion and metastasis of cervical cancer[J]. 中国医药导报, 2022, 19(21): 13-16,25.

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https://www.yiyaodaobao.com.cn/EN/ OR https://www.yiyaodaobao.com.cn/EN/Y2022/V19/I21/13

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