三七总皂苷对A549细胞侵袭转移的影响及机制研究

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Abstract Objective To investigate the effect and mechanism of Panax notoginseng saponins (PNS) on invasion and metastasis of A549 cells. Methods A549 cells were divided into control group, low dose of PNS group (15 mg/L), medium dose of PNS group (30 mg/L), high dose of PNS group (60 mg/L) according to different culture methods. Transwell cell invasion test was used to detect the invasion and metastasis ability of A549 cells, qRT-PCR and Western blot methods were used to detect the expression levels of miRNA-379-5p, matrix metalloproteinase (MMP)-2 and MMP-9 in A549 cells. Results Compared with control group, the invasion and metastasis ability of A549 cells of low, medium, high dose of PNS groups decreased, the expression of miRNA-379-5p increased, the expression of MMP-2 protein and mRNA decreased, the differences were statistically significant (P ＜ 0.05 or P ＜ 0.01); the expression of MMP-9 protein and mRNA of medium and high dose of PNS groups decreased (P ＜ 0.05). Conclusion PNS could inhibit the invasion and metastasis of A549 cells, and its mechanism may be related to upregulation of miRNA-379-5p expression and downregulation of MMP-2 and MMP-9 expression in A549 cells induced by PNS.

Key words： Panax notoginseng saponins Invasion miRNA-379-5p Matrix metalloproteinase-2 Matrix metalloproteinase-9

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	GONG Chunxiang XU Weiwei SHAO Xin

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GONG Chunxiang XU Weiwei SHAO Xin. The research on the effect and mechanism of Panax notoginseng saponins on invasion and metastasis of A549 cells[J]. 中国医药导报, 2018, 15(28): 17-20.

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http://www.yiyaodaobao.com.cn/EN/ OR http://www.yiyaodaobao.com.cn/EN/Y2018/V15/I28/17

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