下调人质膜型唾液酸酶对前列腺癌PC-3细胞增殖、侵袭能力的影响及其机制

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Abstract Objective To investigate the influence of Neu3 gene silencing in growth inhibition of PC-3 cell and to elucidate the underlying mechanisms. Methods Three pieces of siRNA sequences on the Neu3 gene were designed and then a recombinant plasmid carrying siRNA was constructed, which was used to transfer PC-3 cells in order to screen the most effective sequence among the three siRNA sequences against Neu3; PC-3 cell transfection pGCsi-Neu3 plasmid, inverted microscope was used to observe the transfection efficiency, determined by MTT test their effects on cell proliferation and flow cytometry was used to detect the change of cell apoptosis; Transwell migration and invasion assay were performed to detect the migration and invasion cells of groups. Results The siRNA sequences targeted to Neu3 gene were designed and their expression vectors were cloned and verified successfully. Compared with control group, pGCsi-Neu3-3 plasmid significantly inhibited cell proliferation (P < 0.01); pGCsi-Neu3-3 plasmid significantly promoted the apoptosis of PC-3 cells of prostate cancer compared with the blank control group (Mock group), the difference was statistically significant (P < 0.05). pGCsi-Neu3-3 plasmid could inhibit the migration and invasion assay of PC-3 cells of prostate cancer, compared with the control group, with statistically significant difference (P < 0.01). Conclusion Construction, identification and initial study of Neu3, might lay the foundation of experiment for the study of growth and metastasis mechanism of prostate cancer cell.

Key words： Human plasma membrane-associated sialidase Prostate cancer Plasmid construction Proliferation Migration

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	LI Xiaojie JIANG Jun ZHU Bei

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LI Xiaojie JIANG Jun ZHU Bei. Influence of down regulation of Neu3′s to PC-3 cell′s proliferation, invasiveness, and its mechanism[J]. 中国医药导报, 2018, 15(28): 8-12.

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http://www.yiyaodaobao.com.cn/EN/ OR http://www.yiyaodaobao.com.cn/EN/Y2018/V15/I28/8

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