LncRNA MEG3在甲状腺乳头状癌中的表达及对增殖、侵袭与转移能力的影响

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Abstract Objective To investigate the expression of lncRNA MEG3 in papillary thyroid carcinoma and explore the effect of MEG3 on proliferation, migration and invasion in PTC-1 cells. Methods 68 cases of papillary thyroid carcinoma specimens and paired normal tissue were collected after surgery from May 2012 to December 2015 in the Fifth Affiliated Hospital of Zunyi Medical College. The expression of MEG3 was analyzed by quantitative real-time PCR. The effect of MEG3 on proliferation in TPC-1 cells was detected by CCK-8 method. The effect of MEG3 on migration and invasion abilities of TPC-1 cells were analyzed by Transwell assays. The expression of Snail2 was detected by Western blot. Results The expression of MEG3 was lower in papillary thyroid carcinoma tissues than adjacent normal tissues (P < 0.05). The expression of MEG3 was related with TNM stage and tumor size (P < 0.05). Over expressed MEG3 significantly inhibited the proliferation of TPC-1 cells (P < 0.05). And MEG3 could decrease the abilities of migration and invasion by down regulating the expression of Snail2 (P < 0.05). Conclusion The expression of lncRNA MEG3 is lower in the papillary thyroid carcinoma and can inhibit the proliferation, migration and invasion of TPC-1 cells can be inhibited by the expression of Snail2.

Key words： MEG3 Papillary thyroid carcinoma lncRNA

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	CUI Dawei1 JIANG Feng2 CHEN Daxin2

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CUI Dawei1 JIANG Feng2 CHEN Daxin2. The expression of lncRNA MEG3 and the influence of proliferation，migration and invasion in papillary thyroid carcinoma[J]. 中国医药导报, 2017, 14(32): 15-18,42.

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http://www.yiyaodaobao.com.cn/EN/ OR http://www.yiyaodaobao.com.cn/EN/Y2017/V14/I32/15

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