出血性中风1号方对脑出血大鼠脑组织的影响

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Abstract Objective To study the effect of First Prescription for Hemorrhagic Stroke on the brain tissue of rats with intracerebral hemorrhage (ICH). Methods A total of 84 male SD rats with the body weight of (220±20) g were randomly selected and divided into blank group and sham operation group (injected with normal saline) by random number table method, with 12 rats in each group. The remaining 60 rats were treated with ICH model induced by collagase Ⅶ injection, and were divided into model group, Naoxueshu group (2.08 ml/kg), and First Prescription for Hemorrhagic Stroke low-dose, medium-dose, and high-dose groups (3.2, 6.4, 12.8 g/kg) by random number table method. Blank group, sham operation group, and model group were intragastric with equal amount of normal saline, and the other groups were intragastric with corresponding dose of drugs, once a day, for consecutive seven days. After administration, hematoxylin-eosin staining was used to observe the pathological changes of seven groups. The changes of cerebral water content in seven groups were determined by wet and dry weight method. The levels of tumor necrosis factor -α (TNF-α) and interleukin-6 (IL-6) in the seven groups were detected by enzyme linked immunosorbent assay, and the levels of nuclear factor-κB (NB-κB) p65 and phosphorylated NF-κB (p-NF-κB) p65 were detected by Western blot. Results The blank group and the sham operation group had complete brain tissue structure, the model group had a large number of red blood cells and inflammatory cells infiltration, disorder, severe edema, First Prescription for Hemorrhagic Stroke low-dose, medium-dose, and high-dose groups all had a small number of red blood cells. There were no significant differences in cerebral water content, TNF-α, IL-6, and p-NF-κB p65 levels between blank group and sham group (P ＞ 0.05). The cerebral water content, TNF-α, IL-6, and p-NF-κB p65 in model group were higher than those in blank group (P ＜ 0.05). The cerebral water content, TNF-α, IL-6, and p-NF-κB p65 in First Prescription for Hemorrhagic Stroke medium-dose and high-dose groups were lower than those in the model group and First Prescription for Hemorrhagic Stroke low-dose group (P ＜ 0.05). There were no significant differences in cerebral water content, TNF-α, IL-6, and p-NF-κB p65 levels between First Prescription for Hemorrhagic Stroke low-dose group and model group (P ＞ 0.05). There was no significant difference in cerebral water content, TNF-α, IL-6, and p-NF-κB p65 between Hemorrhagic Stroke medium-dose and high-dose groups (P ＞ 0.05). There was no significant difference in NF-κB p65 level among all groups (P ＞ 0.05). Conclusion Medium and high dose of First Prescription for Hemorrhagic Stroke has brain protective effect on ICH rats, which may be related to the inhibition of p-NF-κB P65, TNF-α, and IL-6 expression.

Key words： Intracerebral hemorrhage First Prescription of Hemorrhagic Stroke Inflammatory response Brain edema Nuclear factor-κB

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	ZHANG Jie1 WU Qian1 WANG Xiaoling2 TANG Yong2 WU Zongtao2

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ZHANG Jie1 WU Qian1 WANG Xiaoling2 TANG Yong2 WU Zongtao2. Effect of First Prescription for Hemorrhagic Stroke on the brain tissue of rats with intracerebral hemorrhage[J]. 中国医药导报, 2022, 19(17): 18-21.

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https://www.yiyaodaobao.com.cn/EN/ OR https://www.yiyaodaobao.com.cn/EN/Y2022/V19/I17/18

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