间歇性缺氧小鼠血清外泌体对Lewis肺癌细胞上皮间充质转化及侵袭转移的影响

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Abstract Objective To investigate the effect of serum exosomes in intermittent hypoxia (IH) mice on epithelial-mesenchymal transition, invasion and metastasis of Lewis lung cancer cells (LLC). Methods Eighty C57BL/6J male mice, 7-8 weeks old, weighing 22-25 g were selected. Two mice with similar body weight were randomly divided into indoor air group (RA) and IH group. The difference in average body weight of mice between the two groups was ≤2 g, with 40 mice in each group. In the IH group, IH stimulation was performed for one minute per cycle, and the oxygen partial pressure was changed from (6±1)%/30 s to (21±1)%/30 s, 12 h/d, for four consecutive weeks. Mice in RA group were housed in ordinary cages. Extraction of exosomes from mouse serum; cell viability was detected by CCK-8 method; Transwell assay and cell scratch assay were used to detect cell invasion and migration ability; Western blot was used to detect the protein expression levels of E-cadherin, N-cadherin, and matrix metalloproteinase 9 (MMP-9). Results After incubating LLC cells with mouse serum exosomes, the number of viable cells in the IH group was higher than that in the RA group, and the difference was highly statistically significant (P ＜ 0.01); the ability of cell invasion and migration in the IH group was higher than that in the RA group, and the difference was highly statistically significant (P ＜ 0.01); the expression level of E-cadherin in the IH group was lower than that in the RA group, and the difference was statistically significant (P ＜ 0.05); the expression level of N-cadherin in the IH group was higher than that in the RA group, and the difference was statistically significant (P ＜ 0.05); therei was no significant difference in the expression levels of MMP-9 between the two groups (P ＞ 0.05). Conclusion Serum exosomes from IH mice may induce EMT and invasion and metastasis of LLC cells.

Key words： Intermittent hypoxia Lewis lung carcinoma cell Epithelial-mesenchymal transformation Metastasis

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	CHENG Linjie YAO Qiaoling

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CHENG Linjie YAO Qiaoling. Effects of serum exosomes in intermittent hypoxia mice on epithelial-mesenchymal transformation, invasion and metastasis of Lewis lung cancer cells[J]. 中国医药导报, 2022, 19(17): 5-9.

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https://www.yiyaodaobao.com.cn/EN/ OR https://www.yiyaodaobao.com.cn/EN/Y2022/V19/I17/5

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