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Study on the effects of D-Cycloserine combined with Rifampicin and Isoniazid on drug-resistant Mycobacterium tuberculosis in vitro
CHEN Shengjie1   LI Xi1   LI Chao2▲
1.Department of Clinical Laboratory, the First Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangdong Province, Guangzhou   510405, China; 
2.Department of Central Laboratory, Foshan Women and Children Hospital Affiliated to Southern Medical University, Guangdong Province, Foshan   528000, China
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Abstract  Objective To evaluate the effect of D-Cycloserine (DCS) combined with Rifampicin and Isoniazid on drug-resistant Mycobacterium tuberculosis in vitro. Methods The minimum inhibitory concentration (MIC) of 38 clinical isolates against DCS, Rifampicin and Isoniazid were determined by microporous plate Alamar Blue method, the combined effect of DSC and Rifampicin and Isoniazid in vitro was detected by checkerboard dilution method, and the combined effect was determined by fractional inhibitory concentration index (FICI). Results Seven strains (18.4%) displayed synergistic effect in DCS combination with Rifampicin, and combined MIC of 24 strains (63.2%) reduced several concentration gradients. After combined DSC with Isoniazid, 5 strains (13.2%) showed synergistic effect, and the combined MIC of 22 strains (57.9%) reduced several concentration gradients. No antagonistic effect was observed with either combination drug regimen. MIC50 and MIC90 decreased after DSC combined with Rifampicin and Isoniazid. Conclusion DSC combined with Rifampicin and Isoniazid can reduce the resistance of clinical drug-resistant strains to varying degrees. DCS combined with therapy is beneficial to the treatment of drug-resistant tuberculosis patients.
Key wordsMycobacterium tuberculosis      Drug resistance      The joint drug susceptiblity      Synergistic     
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CHEN Shengjie1 LI Xi1 LI Chao2▲
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CHEN Shengjie1 LI Xi1 LI Chao2▲. Study on the effects of D-Cycloserine combined with Rifampicin and Isoniazid on drug-resistant Mycobacterium tuberculosis in vitro[J]. 中国医药导报, 2022, 19(16): 26-29,37.
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https://www.yiyaodaobao.com.cn/EN/     OR     https://www.yiyaodaobao.com.cn/EN/Y2022/V19/I16/26
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