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| Effect of akupotomye on stiffness and fibrosis of rectus femoris muscle in rabbits with knee osteoarthritis |
| GONG Yurong1* LIN Qiaoxuan2* LIU Jing3 GUO Zexing4 LU Liming4 XIU Zhongbiao3 |
1.Department of Ultrasound Medicine, Affiliated People’s Hospital of Fujian University of Traditional Chinese Medicine, Fujian Province, Fuzhou 350004, China; 2.Department of Orthopedics, the Third People’s Hospital Affiliated to Fujian University of Traditional Chinese Medicine, Fujian Province, Fuzhou 350122, China;
3.the First Department of Orthopedics, Affiliated People’s Hospital of Fujian University of Traditional Chinese Medicine, Fujian Province, Fuzhou 350004, China;
4.College of Traditional Chinese Medicine, Fujian University of Traditional Chinese Medicine, Fujian Province, Fuzhou 350122, China |
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Abstract Objective To explore the effect of akupotomye on stiffness and fibrosis of rectus femoris muscle in rabbits with knee osteoarthritis (KOA). Methods Twenty-four healthy male New Zealand rabbits were divided into blank group, model group, and akupotomye group by random number table method, with eight rabbits in each group. The KOA rabbit model was made by plaster fixation of left hind limb in straight position for six weeks by modified Videman method. The model success criteria was evaluated by the Lequesne MG score of knee joint behavior ≥4 points. Akupotomye group received akupotomye release treatment for “top of the cranium”, “upper of the external iliac”, and “lower of the internal iliac”, intervention once every seven days, a total of four interventions. The blank group and model group underwent the same grasping and fixation, but no akupotomye treatment. One week after the intervention, real-time tissue elastography was performed to measure the elastic strain rate ratio to evaluate the changes in tissue hardness of rectus femoris muscle. Masson staining was used to observe the changes of tissue morphology and collagen fiber content of rectus femoris muscle. Results Compared with the blank group, the elastic strain rate ratio and collagen volume fraction of rectus femoris muscle in the model group were all increased (P ＜ 0.05). Compared with the model group, the ratio of elastic strain rate and collagen volume fraction of rectus femoris in the akupotomye group decreased significantly (P ＜ 0.05). Conclusion Akupotomye intervention can improve the hardness of rectus femoris tissue in KOA rabbits and promote the restore of chronic injury fibrosis of rectus femoris. It can be one of the mechanisms of acupotomy in treating KOA.
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