胰岛素对谷胱甘肽过氧化物酶表达的影响及其意义

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Abstract Objective To explore the effects of insulin on glutathione peroxidase (GSH-Px) expression and its significance. Methods The differentiation of 3T3-L1 preadipocytes were induced by cocktail method and the degree of differentiation was identified by oil red O staining; after 3T3-L1 adipocytes were treated with 500 nmol/L insulin for 1 h, the mRNA expression levels of GSH-PX1, GSH-PX4, and GSH-PX7 were detected by real-time quantitative PCR; 3T3-L1 adipocytes were pretreated with GSH-Px inhibitor Mercaptosuccinate (MS, 10, 20 mmol/L) for 30 min and then stimulated with or without 500 nmol/L insulin for 4 h, the expression level of glucose transporter 4 (GLUT4) protein was detected by Western blot. Results 3T3-L1 preadipocytes were spindle-shaped; and the cells gradually became spheroid and small lipid droplets were observed in the cells on the 5th day after induced differentiation; the cells further became spherical and the number of intracellular lipid droplets increased significantly on the 8th day after induced differentiation. The mRNA relative expression levels of GSH-Px1，GSH-Px4, and GSH-Px7 in insulin group were lower than those in control group, and the differences were statistically significant (P < 0.05); with or without insulin, there was no significant in expression of GLUT4 protein between the 10 mmol/L MS treatment group and the MS untreated group (P ＞ 0.05); the level of GLUT4 protein in 20 mmol/L MS treated group was lower than that in MS untreated group, and the difference was statistically significant (P < 0.05). Conclusion Long-term insulin stimulation inhibited GSH-Px mRNA expression, and MS inhibition of GSH-Px activity negatively regulated GLUT4 protein expression.

Key words： Insulin Glucose transporter 4 3T3-L1 adipocytes Glutathione peroxidase

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	MA Jinhui1 LIU Jie1 LI Yuanyuan2 SONG Yankun3 ZHENG Lishuang4 TAN Yinghui4 XI Xin4

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MA Jinhui1 LIU Jie1 LI Yuanyuan2 SONG Yankun3 ZHENG Lishuang4 TAN Yinghui4 XI Xin4. Effects of insulin on glutathione peroxidase expression and its significance[J]. 中国医药导报, 2022, 19(15): 4-7.

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https://www.yiyaodaobao.com.cn/EN/ OR https://www.yiyaodaobao.com.cn/EN/Y2022/V19/I15/4

[1] Khalid M，Alkaabi J，Khan MAB，et al. Insulin Signal Transduction Perturbations in Insulin Resistance [J]. Int J Mol Sci，2021，22（16）：8590.
[2] Yaribeygi H，Farrokhi FR，Butler AE，et al. Insulin resistance：Review of the underlying molecular mechanisms [J]. J Cell Physiol，2019，234（6）：8152-8161.
[3] da Silva Rosa SC，Nayak N，Caymo AM，et al. Mechanisms of muscle insulin resistance and the cross-talk with liver and adipose tissue [J]. Physiol Rep，2020，8（19）：e14607.
[4] Sampath Kumar A，Maiya AG，Shastry BA，et al. Exercise and insulin resistance in type 2 diabetes mellitus：A systematic review and meta-analysis [J]. Ann Phys Rehabil Med，2019，62（2）：98-103.
[5] Klip A，McGraw TE，James DE. Thirty sweet years of GLUT4 [J]. J Biol Chem，2019，294（30）：11369-11381.
[6] Wang T，Wang J，Hu X，et al. Current understanding of glucose transporter 4 expression and functional mechanisms [J]. World J Biol Chem，2020，11（3）：76-98.
[7] Li DT，Habtemichael EN，Julca O，et al. GLUT4 Storage Vesicles：Specialized Organelles for Regulated Trafficking [J]. Yale J Biol Med，2019，92（3）：453-470.
[8] 姚欢欢，陈吉，陈思思，等.基于AMPK/GLUT4/GSK3β/PPARα信号通路研究地骨皮水提物改善2型糖尿病大鼠胰岛素抵抗的实验研究[J].中国医药导报，2020，17（5）：8-12.
[9] Cheng YC，Chiu YM，Dai ZK，et al. Loganin Ameliorates Painful Diabetic Neuropathy by Modulating Oxidative Stress，Inflammation and Insulin Sensitivity in Streptozotocin-Nicotinamide-Induced Diabetic Rats [J]. Cells，2021， 10（10）：2688.
[10] Rehman K，Akash MSH. Mechanism of Generation of Oxidative Stress and Pathophysiology of Type 2 Diabetes Mellitus：How Are They Interlinked [J]. J Cell Biochem，2017，118（11）：3577-3585.
[11] Grotle AK，Stone AJ. Exaggerated exercise pressor reflex in type 2 diabetes：Potential role of oxidative stress [J]. Auton Neurosci，2019，222：102591.
[12] Ruegsegger GN，Creo AL，Cortes TM，et al. Altered mitochondrial function in insulin-deficient and insulin-resistant states [J]. J Clin Invest，2018，128（9）：3671-3681.
[13] Flores-Riveros JR，McLenithan JC，Ezaki O，et al. Insulin down-regulates expression of the insulin-responsive glucose transporter（GLUT4）gene：effects on transcription and mRNA turnover [J]. P Natl Acad Sci U S A，1993，90（2）：512-516.
[14] Sargeant RJ，P?覾quet MR. Effect of insulin on the rates of synthesis and degradation of GLUT1 and GLUT4 glucose transporters in 3T3-L1 adipocytes [J]. Biochem J，1993，290（3）：913-919.
[15] Pankov YA. Adipogenic function and other biologic effects of insulin [J]. Biomed Khim，2016，62（1）：5-13.
[16] Ma J，Nakagawa Y，Kojima I，et al. Prolonged insulin stimulation down-regulates GLUT4 through oxidative stress-mediated retromer inhibition by a protein kinase CK2-dependent mechanism in 3T3-L1 adipocytes [J]. J Biol Chem，2014，289（1）：133-142.
[17] Ma M，Quan Y，Li Y，et al. Bidirectional modulation of insulin action by reactive oxygen species in 3T3-L1 adipocytes [J]. Mol Med Rep，2018，18（1）：807-814.
[18] Mahadev K，Motoshima H，Wu X，et al. The NAD（P）H oxidase homolog Nox4 modulates insulin-stimulated generation of H2O2 and plays an integral role in insulin signal transduction [J]. Mol Cell Biol，2004，24（5）：1844-1854.
[19] Walton EL. Oxidative stress and diabetes：Glucose response in the cROSsfire [J]. Biomed J，2017，40（5）：241-244.
[20] de Vega RG，Fernández-Sánchez ML，Fernández JC，et al. Selenium levels and glutathione peroxidase activity in the plasma of patients with type Ⅱ diabetes mellitus [J]. J Trace Elem Med Biol，2016，37：44-49.
[21] Li D，Jiang C，Mei G，et al. Quercetin Alleviates Ferroptosis of Pancreatic β Cells in Type 2 Diabetes [J]. Nutrients，2020，12（10）：2954.
[22] Ling P，Shan W，Zhai G，et al. Association between glutathione peroxidase-3 activity and carotid atherosclerosis in patients with type 2 diabetes mellitus [J]. Brain Behav，2020，10（10）：e01773.
[23] Steyn M，Zitouni K，Kelly FJ，et al. Sex Differences in Glutathione Peroxidase Activity and Central Obesity in Patients with Type 2 Diabetes at High Risk of Cardio-Renal Disease [J]. Antioxidants（Basel），2019，8（12）：629.
[24] Zhao X，Hu H，Wang C，et al. A comparison of methods for effective differentiation of the frozen-thawed 3T3-L1 cells [J]. Anal Biochem，2019，568：57-64.
[25] Liu LB，Omata W，Kojima I，et al. The SUMO conjugating enzyme Ubc9 is a regulator of GLUT4 turnover and targeting to the insulin-responsive storage compartment in 3T3-L1 adipocytes [J]. Diabetes，2007，56（8）：1977-1985.