SCNN1A多态性及其编码蛋白表达与子痫前期的相关性分析

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Abstract Objective To investigate the expression and significance of SCNN1A polymorphism and its coding protein in placenta tissues of Uygur ethnic group pregnant women with preeclampsia. Methods A total of 122 Uyghur ethnic group pregnant women with preeclampsia who delivered or terminated their pregnancy in People’s Hospital of Xinjiang Uygur Autonomous Region (hereinafter referred to as “our hospital”) from January 2017 to December 2019 were selected as the case group; according to the time limit of onset, the patients were divided into early onset preeclampsia group (onset of gestational age < 34 weeks, 50 cases) and late onset preeclampsia group (onset of gestational age ≥34 weeks, 72 cases). At the same time, 125 normal pregnant women who delivered to term in our hospital during the same period were selected as the control group. By collecting placental tissue from both groups, DNA, RNA, and protein were extracted from the placenta tissues of the patients, and the longest exon 13 polymorphism of SCNN1A was detected by first-generation sequencing. Real-time PCR was used to detect α-ENaC mRNA transcription level, and Western blot was used to detect α-ENaC protein expression level. The correlation between α-ENaC protein expression level and renal function index was analyzed. Results There was only one polymorphic site—T663A, among the six mutation sites in exon 13 of SCNN1A gene in the two groups. There was no significant difference in T663A polymorphic loci gene type between two groups (P > 0.05). Genotypes and alleles of T663A polymorphic loci showed no significant difference amang all groups (P > 0.05). There was no significant difference in α-ENaC expression among different T663A polymorphic loci genotypes (P > 0.05). There were no significant differences in α-ENaC mRNA and protein among all groups (P > 0.05). The relative expression of α-ENaC protein was negatively correlated with urinary protein (r = -0.846, P < 0.05). Conclusion α-ENaC protein expression is related to renal function impairment in pregnant women.

Key words： SCNN1A Epithelial Na+ channel Preeclampsia Uygur ethnic group Time limit of onset

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	SUI Shuang FENG Guohui HUANG Ying LI Xiaoying

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SUI Shuang FENG Guohui HUANG Ying LI Xiaoying. Correlation analysis of SCNN1A polymorphism and its encoding protein expression with preeclampsia#br#[J]. 中国医药导报, 2022, 19(13): 29-32.

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https://www.yiyaodaobao.com.cn/EN/ OR https://www.yiyaodaobao.com.cn/EN/Y2022/V19/I13/29

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