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| Study of quality control method of Wenyang Fuzheng Powder based on gas chromatography fingerprint and gas chromatography-mass spectrometry identification |
| YANG Sicheng1 HUANG Ju1 ZHOU Zhongyu2 HUANG Wei2 CHEN Shuhe3 DUAN Xueyun3 LIU Guoxiu4 ZHAI Huaqiang4 SUN Wanjin3 HUANG Zhengde3 |
1.School of Pharmacy, Hubei University of Chinese Medicine, Hubei Province, Wuhan 430065, China;
2.Department of Acupuncture, Hubei Hospital of Traditional Chinese Medicine Affiliated Hospital of Hubei University of Chinese Medicine Hubei Province Academy of Traditional Chinese Medicine, Hubei Province, Wuhan 430061, China;
3.Department of Pharmaceutical Affairs, Hubei Hospital of Traditional Chinese Medicine Affiliated Hospital of Hubei University of Chinese Medicine Hubei Province Academy of Traditional Chinese Medicine, Hubei Province, Wuhan 430061, China;
4.Standardization Research Center of Traditional Chinese Medicine Dispensing, Beijing University of Chinese Medicine, Beijing 102488, China |
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Abstract Objective To establish a quality control model of Wenyang Fuzheng Powder as a representative prescription containing volatile components based on the identification of gas chromatography (GC) fingerprint and gas chromatography-mass spectrometry (GC-MS) analysis technology, and to provide a basis for the standard study of powder. Methods The volatile oil in Wenyang Fuzheng Powder was extracted by the volatile oil determination method and analyzed by GC-MS technology on the basis of the establishment of GC fingerprint. An Elite-WAX ETR (max 250℃, 0.25 μm×0.25 mm×30 m) capillary column was used in this experiment. The GC conditions: the heating program was set as the initial temperature of 50℃, holding time of one minutes, heating to 180℃ at a rate of 10℃/min, holding time of three minutes, then heating to 240℃ at a rate of 10℃/min, holding time of five min. The carrier gas was high-purity nitrogen (≥ 99.999%), and the carrier gas flow rate was 1.0 ml/min, the shunt ratio was set as 20∶1, the detector was FID, the detector temperature was 280℃, and the injector temperature was 200℃. The GC-MS conditions: electron impact ion source, bombarding voltage 70 eV, positive ion mode, ion source temperature was 230℃, interface temperature was 220℃; the scanning range of MS was m/z 30-500 amu; carrier gas was high purity helium (≥99.999%). Results Through the analysis of volatile oil of ten batches of Wenyang Fuzheng Powder, 12 common peaks were obtained, the fingerprint of volatile components of Wenyang Fuzheng Powder was established and the qualitative study was carried out. The quality and quantity of the main volatile components in this formula could be repeated and stable in different batches, and this model could be used for quality control research. Conclusion In this study, a quality control model of Wenyang Fuzheng Powder is established. The method is simple, safe and reliable, and provides a basis for quality evaluation of Wenyang Fuzheng Powder.
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| Cite this article: |
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YANG Sicheng1 HUANG Ju1 ZHOU Zhongyu2 HUANG Wei2 CHEN Shuhe3 DUAN Xueyun3 LIU Guoxiu4 ZHAI Huaqiang4 SUN Wanjin3 HUANG Zhengde3. Study of quality control method of Wenyang Fuzheng Powder based on gas chromatography fingerprint and gas chromatography-mass spectrometry identification[J]. 中国医药导报, 2022, 19(13): 20-24.
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| URL: |
| https://www.yiyaodaobao.com.cn/EN/ OR https://www.yiyaodaobao.com.cn/EN/Y2022/V19/I13/20 |
[1] 范萍，龙翔宇，王刚，等.麦粒灸调理阳虚质亚健康人群的疗效观察[J].名医，2019（2）：10-11.
[2] 茹江丽，李静，李旭.亚健康人群阳虚体质研究进展[J].北京中医药，2021，40（2）：214-216.
[3] 徐璡，许朝霞，王又闻，等.亚健康状态中医证候特征研究进展[J].中华中医药杂志，2016，31（4）：1356-1358.
[4] 孔令晶，陶国水，陆曙.亚健康状态的中医临床诊治经验[J].中医药临床杂志，2019，31（12）：2252-2255.
[5] 巫柳萍，余连香，赵琨，等.改进后督灸治疗阳虚质亚健康的临床疗效观察[J].中医临床研究，2020，12（31）：64-67.
[6] 高振雷.吴茱萸化学成分及药理作用研究概况[J].科学技术创新，2020（13）：53-54.
[7] 魏舒婷，刘元乾，黄坚，等.吴茱萸化学成分、药效及肝毒性的研究进展[J].世界中医药，2020，15（23）：3580-3585， 3592.
[8] 尹利顺，孙蓉.吴茱萸挥发油化学成分与药理毒理研究进展[J].中国药物警戒，2016，13（3）：162-164.
[9] 艾勇，朱思阳，艾艳.肉桂挥发油的提取方法与抗炎镇痛作用研究[J].广东化工，2020，47（15）：50-53，56.
[10] 张笮晦，童永清，黄广智，等.肉桂叶化学成分及药理作用研究进展[J].广州化工，2019，47（1）：20-22.
[11] 陈旭，刘畅，马宁辉，等.肉桂的化学成分、药理作用及综合应用研究进展[J].中国药房，2018，29（18）：2581-2584.
[12] 邓淑蓉，潘宇政.肉桂主要化学成分及药理作用研究概况[J].现代中西医结合杂志，2018，27（4）：448-451.
[13] 李莎莎，李凡，李芳，等.丁香的化学成分与药理作用研究进展[J].西北药学杂志，2021，36（5）：863-868.
[14] 美丽，朱懿敏，罗晶，等.丁香化学成分、药效及临床应用研究进展[J].中国实验方剂学杂志，2019，25（15）：222-227.
[15] 亓雪，张颖颖.干姜的化学、药理研究进展[J].山东化工，2018，47（14）：41-42.
[16] 王文心.干姜的化学、药理及临床应用特点分析[J].中医临床研究，2016，8（6）：146-148.
[17] 崔婉华，王彦志，李泽之.干姜的化学成分研究[J].中国药学杂志，2018，53（14）：1160-1164.
[18] 孙晓彤，刘善新，苏酩，等.丁香酚透皮吸收的研究进展[J].中国药房，2016，27（11）：1579-1581.
[19] 赵婷婷，张彤，项乐源，等.当归、丁香挥发油的促透皮吸收作用[J].中成药，2016，38（9）：1923-1929.
[20] 国家药典委员会.中华人民共和国药典[M].四部.北京：中国医药科技出版社，2020：233.
[21] 李斐，周立分，董嘉皓，等.GC-MS法测定分析吴茱萸挥发油的有效化学成分[J].南华大学学报（自然科学版），2017，31（3）：101-105.
[22] 王波，龚伟，陈国宝，等.肉桂挥发性成分的气相色谱/质谱分析[J].世界中西医结合杂志，2014，9（9）：941-943.
[23] 李升，郭楚君，张燕，等.气相色谱-质谱技术分析中药丁香挥发油成分[J].黑龙江医药科学，2016，39（4）：8-9.
[24] 张国裕，李淑贤.中药干姜挥发油化学成分的GC-MS分析[J].黑龙江医药科学，2021，44（4）：19-20.
[25] 柳阳，杨艳芳，洪宗超，等.广西莪术挥发油气相色谱指纹图谱研究[J].湖北中医药大学学报，2018，20（4）：48-51.
[26] 高小恒，李莎莎，陈达艳，等.玉红膏挥发油化学成分GC-MS分析[J].西部中医药，2020，33（1）：60-62.
[27] 潘春林，范恒.姜黄挥发油成分气相指纹图谱研究[J].湖北中医药大学学报，2018，20（4）：52-55. |
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