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Study of quality control method of Wenyang Fuzheng Powder based on gas chromatography fingerprint and gas chromatography-mass spectrometry identification |
YANG Sicheng1 HUANG Ju1 ZHOU Zhongyu2 HUANG Wei2 CHEN Shuhe3 DUAN Xueyun3 LIU Guoxiu4 ZHAI Huaqiang4 SUN Wanjin3 HUANG Zhengde3 |
1.School of Pharmacy, Hubei University of Chinese Medicine, Hubei Province, Wuhan 430065, China;
2.Department of Acupuncture, Hubei Hospital of Traditional Chinese Medicine Affiliated Hospital of Hubei University of Chinese Medicine Hubei Province Academy of Traditional Chinese Medicine, Hubei Province, Wuhan 430061, China;
3.Department of Pharmaceutical Affairs, Hubei Hospital of Traditional Chinese Medicine Affiliated Hospital of Hubei University of Chinese Medicine Hubei Province Academy of Traditional Chinese Medicine, Hubei Province, Wuhan 430061, China;
4.Standardization Research Center of Traditional Chinese Medicine Dispensing, Beijing University of Chinese Medicine, Beijing 102488, China |
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Abstract Objective To establish a quality control model of Wenyang Fuzheng Powder as a representative prescription containing volatile components based on the identification of gas chromatography (GC) fingerprint and gas chromatography-mass spectrometry (GC-MS) analysis technology, and to provide a basis for the standard study of powder. Methods The volatile oil in Wenyang Fuzheng Powder was extracted by the volatile oil determination method and analyzed by GC-MS technology on the basis of the establishment of GC fingerprint. An Elite-WAX ETR (max 250℃, 0.25 μm×0.25 mm×30 m) capillary column was used in this experiment. The GC conditions: the heating program was set as the initial temperature of 50℃, holding time of one minutes, heating to 180℃ at a rate of 10℃/min, holding time of three minutes, then heating to 240℃ at a rate of 10℃/min, holding time of five min. The carrier gas was high-purity nitrogen (≥ 99.999%), and the carrier gas flow rate was 1.0 ml/min, the shunt ratio was set as 20∶1, the detector was FID, the detector temperature was 280℃, and the injector temperature was 200℃. The GC-MS conditions: electron impact ion source, bombarding voltage 70 eV, positive ion mode, ion source temperature was 230℃, interface temperature was 220℃; the scanning range of MS was m/z 30-500 amu; carrier gas was high purity helium (≥99.999%). Results Through the analysis of volatile oil of ten batches of Wenyang Fuzheng Powder, 12 common peaks were obtained, the fingerprint of volatile components of Wenyang Fuzheng Powder was established and the qualitative study was carried out. The quality and quantity of the main volatile components in this formula could be repeated and stable in different batches, and this model could be used for quality control research. Conclusion In this study, a quality control model of Wenyang Fuzheng Powder is established. The method is simple, safe and reliable, and provides a basis for quality evaluation of Wenyang Fuzheng Powder.
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Cite this article: |
YANG Sicheng1 HUANG Ju1 ZHOU Zhongyu2 HUANG Wei2 CHEN Shuhe3 DUAN Xueyun3 LIU Guoxiu4 ZHAI Huaqiang4 SUN Wanjin3 HUANG Zhengde3. Study of quality control method of Wenyang Fuzheng Powder based on gas chromatography fingerprint and gas chromatography-mass spectrometry identification[J]. 中国医药导报, 2022, 19(13): 20-24.
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URL: |
https://www.yiyaodaobao.com.cn/EN/ OR https://www.yiyaodaobao.com.cn/EN/Y2022/V19/I13/20 |
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