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| Effect of Bushen Shengxue Decoction on bone marrow cells in the early stage of chemotherapy |
| WU Rong1 ZHU Wei1 WANG Wenjuan1 CHI Li1 JIA Chunrong2 YUE Zhujun3 |
1.School of Traditional Chinese Medicine, Capital Medical University, Beijing 100069, China;
2.Department of Traditional Chinese Medicine, Beijing Tiantan Hospital, Capital Medical University, Beijing 100051, China;
3.Department of Pathology, Beijing Youan Hospital, Capital Medical University, Beijing 100069, China |
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Abstract Objective To observe the effect of Bushen Shengxue Decoction on bone marrow cells in the early stage of chemotherapy. Methods Sixty-five male SPF class BALB/c mice aged six to eight weeks with body weight of (20±2) g were selected and divided into blank group, model group, positive control group, high dose Bushen Shengxue Decoction group, and low dose Bushen Shengxue Decoction group according to random number table method, with 13 mice in each group. For five days of preventive administration, the high (47.0 g/kg) and low (23.5 g/kg) dose Bushen Shengxue Decoction groups were given intragastric administration, while the other groups were given intragastric administration with equal amount of distilled water. After preventive administration, Cyclophosphamide (100 mg/kg) was intraperitoneally injected into model group, positive control group, high and low dose Bushen Shengxue Decoction groups, and the same amount of normal saline was intraperitoneally injected into blank group for consecutive three days. At the same time, the positive control group was subcutaneously injected with recombinant human granulocyte colony stimulating factor (30 μg/kg), and the other groups were given the same prophylactic administration for consecutive five days. After the intervention, bone marrow cells of each group were collected, and ultrastructure of the cells was observed. The contents of tumor necrosis factor -α (TNF-α), transforming growth factor -β (TGF-β), and interferon-γ (IFN-γ) in bone marrow were detected. The mRNA expression of JAK kinase 2 (JAK2), signal transduction and transcription activator 5 (STAT5), and the protein expression of JAK2, STAT5, and phosphorylated STAT5 (P-STAT5) in bone marrow cells were detected. Results In blank group, the distribution of nucleated cells was dense, the nuclear membrane and cell membrane were intact, and the organelle structure was clear. There were few nuclear cells in the model group and a large number of cell fragments. The number of nuclear cells in the other groups increased to varying degrees, while the cell body decreased and the organelles were good. The contents of TNF-α, TGF-β, and IFN-γ in the model group were higher than those in the blank group, the contents of TNF-α, TGF-β, and IFN-γ in the high and low dose Bushen Shengxue Decoction groups were lower than those in the model group, and the contents of TGF-β and IFN-γ in the high dose Bushen Shengxue Decoction group were lower than those in the low dose Bushen Shengxue Decoction group, the differences were all statistically significant (P ＜ 0.05 or P ＜ 0.01). Compared with blank group, the mRNA expression of JAK2 and STAT5 in model group was down-regulated. Compared with model group, STAT5 mRNA expression in the high and low dose Bushen Shengxue Decoction groups was up-regulated. Compared with the low dose Bushen Shengxue Decoction group, the expression of JAK2 mRNA in the high dose Bushen Shengxue Decoction group was down-regulated, the differences were all statistically significant (P ＜ 0.05 or P ＜ 0.01). The expression levels of JAK2, P-STAT5, and STAT5 protein in the model group were lower than those in the blank group, the expression levels of JAK2 and P-STAT5 protein in the high and low dose Bushen Shengxue Decoction groups were higher than those in the model group, the expression level of P-STAT5 protein in the high dose Bushen Shengxue Decoction group was lower than that in the low dose Bushen Shengxue Decoction group, the differences were statistically significant (P ＜ 0.05 or P ＜ 0.01). Conclusion Bushen Shengxue Decoction can alleviate bone marrow cell damage in the early stage of chemotherapy, and the mechanism is related to the reduction of hematopoietic negative regulatory factor content and positive regulation of JAK2-STAT5 pathway.
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