胃癌组织中脂肪酶H的表达情况及临床意义

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Abstract Objective To study the expression of lipase member H (LIPH) in gastric cancer and its clinical significance. Methods Normal gastric mucosa cells GES-1 and gastric cancer cell lines SGC-7901, AGS, MGC-803, BGC-823, and MKN-45 were selected, and the expression of LIPH in them was detected by Western blot. A total of 95 gastric cancer patients admitted to the First Affiliated Hospital of Bengbu Medical College from January 2014 to December 2015 were selected. Western blot and immunohistochemistry were used to detect LIPH expression levels in cancer tissues and adjacent tissues. LIPH was divided into positive group and negative group according to the expression of LIPH. The two groups were followed up for 60 months, and the clinical significance of LIPH in gastric cancer was analyzed by survival curve and Cox regression. Results The LIPH expression of gastric cancer cell lines MGC-803, AGS, MKN-45, BGC-823, and SGC-7901 was higher than that of normal gastric mucosa cells GES-1, and the differences were statistically significant (P ＜ 0.05). Immunohistochemical results showed that LIPH positive expression in gastric cancer tissues was brown or tan. The positive rate of LIPH expression in cancer tissues was higher than that in adjacent tissues, and the difference was statistically significant (P ＜ 0.05). The cumulative survival rate of 95 patients was 22.1%, the cumulative survival rate of positive group (56 cases) was lower than that of negative group (39 cases), the difference was statistically significant (P ＜ 0.05). Univariate analysis showed that LIPH expression, invasion depth, lymph node metastasis, TNM stage, and differentiation degree were correlated with prognosis of gastric cancer (P ＜ 0.05). Multivariate analysis showed that negative LIPH expression, invasion depth T1-T2, no lymph node metastasis, and TNM stage Ⅰ were prognostic protective factors for gastric cancer patients (HR ＜ 1, P ＜ 0.05). Conclusion LIPH is highly expressed in gastric cancer, which is related to the prognosis of gastric cancer.

Key words： Lipase member H Gastric cancer Clinicopathological features Prognosis

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	YU Dajun LI Zhixiang WANG Kai

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YU Dajun LI Zhixiang WANG Kai. Expression of lipase member H in gastric cancer and its clinical significance#br#[J]. 中国医药导报, 2022, 19(12): 21-25.

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https://www.yiyaodaobao.com.cn/EN/ OR https://www.yiyaodaobao.com.cn/EN/Y2022/V19/I12/21

[1] Siegel RL，Miller KD，Fuchs HE，et al. Cancer statistics，2021 [J]. CA Cancer J Clin，2021，71（1）：7-33.
[2] Bray F，Ferlay J，Soerjomataram I，et al. Global cancer statistics 2018：GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries [J]. CA Cancer J Clin，2018，68（6）：394-424.
[3] Sun Y，Jiang Y，Huang J，et al. CISD2 enhances the chemo-sensitivity of gastric cancer through the enhancement of 5-FU-induced apoptosis and the inhibition of autophagy by AKT/mTOR pathway [J]. Cancer Med，2017，6（10）：2331-2346.
[4] Wang L，Ouyang F，Liu X，et al. Overexpressed CISD2 hasprognostic value in human gastric cancer and promotes gastric cancer cell proliferation and tumorigenesis via AKT signaling pathway [J]. Oncotarget，2016，7（4）：3791-3805.
[5] Mizukami Y，Hayashi R，Tsuruta D，et al. Novel splice site mutation in the LIPH gene in a patient with autosomal recessive woolly hair/hypotrichosis：Case report and published work review [J]. J Dermatol，2018，45（5）：613-617.
[6] Ahmad F，Sharif S，Furqan Ubaid M，et al. Novel sequence variants in the LIPH and LPAR6 genes underlies autosomal recessive woolly hair/hypotrichosis in consanguineous families [J]. Congenit Anom（Kyoto），2018，58（1）：24-28.
[7] Matsuo Y，Tanaka A，Shimomura Y，et al. Novel splice site mutation in LIPH identified in a Japanese patient with autosomal recessive woolly hair [J]. J Dermatol，2016，43（11）：1384-1385.
[8] Zhang Y，Zhu X，Qiao X，et al. LIPH promotes metastasis by enriching stem-like cells in triple-negative breast cancer [J]. J Cell Mol Med，2020，24（16）：9125-9134.
[9] Li Y，Zhou X，Zhang Q，et al. Lipase member H is a downstream molecular target of hypoxia inducible factor-1α and promotes papillary thyroid carcinoma cell migration in BCPAP and KTC-1 cell lines [J]. Cancer Manag Res，2019，11：931-941.
[10] Gui X，Li Y，Zhang X，et al. Circ-LDLR promoted the development of papillary thyroid carcinoma via regulating miR-195-5p/LIPH axis [J]. Cancer Cell Int，2020， 20：241-245.
[11] Zhuang H，Chen X，Wang Y，et al. Identification of LIPH as an unfavorable biomarkers correlated with immune suppression or evasion in pancreatic cancer based on RNA-seq [J]. Cancer Immunol Immunother，2021，7：1-5.
[12] 王伟，周颖，蒋桂英，等.XPNPEP2在胃癌组织中的表达及其临床意义[J].实用医学杂志，2019，35（9）：1393-1396.
[13] 宋早智，王庆康，孙家和，等.DOT1L在胃癌组织中的表达及其与预后的相关性[J].实用医学杂志，2019，35（22）：3498-3502.
[14] Matsuzaki J，Tsugawa H，Suzuki H. Precision Medicine Approaches to Prevent Gastric Cancer [J]. Gut Liver，2021，15（1）：3-12.
[15] Kanda M. Preoperative predictors of postoperative complications after gastric cancer resection [J]. Surg Today，2020，50（1）：3-11.
[16] Jin W，Broedl UC，Monajemi H，et al. Lipase H，a new member of the triglyceride lipase family synthesized by the intestine [J]. Genomics，2002，80（3）：268-273.
[17] Lo PK，Yao Y，Lee JS，et al. LIPG signaling promotes tumor initiation and metastasis of human basal-like triple-negative breast cancer [J]. Elife，2018，7：1-31.
[18] Zhang Y，Zhu X，Qiao X，et al. LIPH promotes metastasis by enriching stem-like cells in triple-negative breast cancer [J]. J Cell Mol Med，2020，24（16）：9125-9134.
[19] Seki Y，Yoshida Y，Ishimine H，et al. Lipase member H is a novel secreted protein selectively upregulated in human lung adenocarcinomas and bronchioloalveolar carcinomas [J]. Biochem Biophys Res Commun，2014，443（4）：1141-1147.
[20] Galluzzi L，Goubar A，Olaussen KA，et al. Prognostic value of LIPC in non-small cell lung carcinoma [J]. Cell Cycle，2013，12（4）：647-654.
[21] Ishimine H，Zhou R，Sumitomo K，et al. Lipase member H frequently overexpressed in human esophageal adenocarcinomas [J]. Tumour Biol，2016，37（2）：2075-2081.
[22] Chen Q，Gao Y，Yu Q，et al. miR-30a-3p inhibits the proliferation of liver cancer cells by targeting DNMT3a through the PI3K/AKT signaling pathway [J]. Oncol Lett，2020，19（1）：606-614.
[23] Lu H，Fu C，Kong S，et al. Maltol prevents the progression of osteoarthritis by targeting PI3K/Akt/NF-κB pathway：In vitro and in vivo studies [J]. J Cell Mol Med，2021，25（1）：499-509.
[24] Wang Y，Lin Y，Wang L，et al. TREM2 ameliorates neuroinflammatory response and cognitive impairment via PI3K/AKT/FoxO3a signaling pathway in Alzheimer’s disease mice [J]. Aging（Albany NY），2020，12（20）：20862-20879.
[25] Ma Z，Lou S，Jiang Z. PHLDA2 regulates EMT and autophagy in colorectal cancer via the PI3K/AKT signaling pathway [J]. Aging（Albany NY），2020，12（9）：7985-8000.