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| Effect of abdominal vibration on the projection temperature of uterus surface and prostaglandin E2 and prostaglandin F2α of cold coagulation dysmenorrhea rats#br# |
| GENG Nan1 HU Hui1 XIE Dandan1 ZHAO Siyu1 WU Fan2 SHEN Qian1 |
1.Dongfang Hospital, Beijing University of Chinese Medicine, Beijing 100078, China;
2.College of Special Education, Beijing Union University, Beijing 100075, China |
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Abstract Objective To investigate the effect of abdominal vibration on the projection temperature of uterus surface and prostaglandin E2 and prostaglandin F2α of cold coagulation dysmenorrhea rats. Methods Thirty SD female six-week-old rats with body weight of (230±20) g were divided into control group, model group, and abdominal vibration group with ten rats in each group by random number table method. Model preparation was performed in model group and abdominal vibration group by freezing combined with subcutaneous injection of Estradiol Benzoate and intraperitoneal injection of Oxytocin combined with pain, while control group was injected with the same dose of normal saline. After the model was successfully prepared, abdominal vibration instrument was used to simulate the abdominal vibration method for treatment. After that, the writhing response of rats and the projection temperature of uterine surface were observed, and the levels of prostaglandin F2α and prostaglandin E2 in serum were detected by enzyme linked immunosorbent assay. Results The writhing incubation period of the model group was shorter than that of the control group, the writhing number of the model group was more than that of the control group, and the writhing score of the model group was higher than that of the control group, and the differences were highly statistically significant (P ＜ 0.01). The writhing incubation period of the abdominal vibration group were longer than those of the model group, the writhing number of the abdominal vibration group was less than that in the model group, and the writhing score of the abdominal vibration group was lower than that of the model group, and the differences were statistically significant (P ＜ 0.05 or P ＜ 0.01). The uterine surface projection temperature in the model group was lower than that in the control group and the uterine surface projection temperature in the abdominal vibration group was higher than that in the model group, and the differences were statistically significant (P ＜ 0.05 or P ＜ 0.01). Serum prostaglandin F2α in model group was higher than that in control group, and serum prostaglandin E2 was lower than that in control group, the differences were statistically significant (P ＜ 0.05 or P ＜ 0.01). The serum prostaglandin F2α in abdominal vibration group was lower than that in model group, and the serum prostaglandin E2 was higher than that in model group, and the differences were statistically significant (P ＜ 0.05 or P ＜ 0.01). Conclusion Abdominal vibration can improve the local temperature imbalance of the uterus, regulate the level of prostaglandin in serum, inhibit the spasm and contraction of the smooth muscle of the uterus, improve local tissue ischemia and hypoxia, and thus achieve the effect of “warming womb and channels and relieving pain”.
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